KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
L-phenylalanine | - |
Escherichia coli | |
0.62 | - |
L-tyrosine | - |
Escherichia coli | |
2.5 | - |
2-oxoglutarate | - |
Escherichia coli | |
3.13 | - |
oxaloacetate | - |
Escherichia coli | |
10 | - |
L-tryptophan | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88000 | - |
- |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + 2-oxoglutarate | - |
Escherichia coli | phenylpyruvate + L-glutamate | - |
? | |
L-tryptophan + 2-oxoglutarate | - |
Escherichia coli | 3-indole-2-oxopropanoate + L-glutamate | - |
r | |
L-tyrosine + 2-oxoglutarate | - |
Escherichia coli | p-hydroxyphenylpyruvate + L-glutamate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000-45000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
10 min, less than 10% of original activity towards tyrosine and phenylalanine | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
tryptophan | Escherichia coli |
8 | - |
with tyrosine | Escherichia coli |
8 | - |
with phenylalanine | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | approx. 45% of maximal activity at pH 6.0,: approx. 60% of maximal activity at pH 9.5, phenylalanine | Escherichia coli |
6 | 10 | approx. 40% of maximal activity at pH 6.0, tryptophan | Escherichia coli |
6 | 10 | approx. 25% of maximal activity at pH 10.0, tryptophan | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | Km: 0.01 mM | Escherichia coli | |
pyridoxal 5'-phosphate | a pyridoxal phosphate protein | Escherichia coli |