BRENDA - Enzyme Database show
show all sequences of 2.6.1.54

Studies on vitamin b6 metabolism in microorganisms. Part X. Further purification and characterization of pyridoxamine 5'-phosphate-alpha-ketoglutarate transaminase from Clostridium kainantoi

Tani, Y.; Ukita, M.; Ogata, K.; Agric. Biol. Chem. 36, 181-188 (1972)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
4-aminobutyrate
stimulation, 177% of non-stimulated activity
Clostridium butyricum
D-asparagine
stimulation, 112% of non-stimulated activity
Clostridium butyricum
D-cysteine
stimulation, 135% of non-stimulated activity
Clostridium butyricum
D-methionine
stimulation, 127% of non-stimulated activity
Clostridium butyricum
DL-Ornithine
stimulation, 112% of non-stimulated activity
Clostridium butyricum
glycine
stimulation, 137% of non-stimulated activity
Clostridium butyricum
L-asparagine
stimulation, 112% of non-stimulated activity
Clostridium butyricum
L-glutamate
stimulation, 166% of non-stimulated activity
Clostridium butyricum
L-lysine
stimulation, 105% of non-stimulated activity
Clostridium butyricum
L-methionine
stimulation, 114% of non-stimulated activity
Clostridium butyricum
L-ornithine
stimulation, 112% of non-stimulated activity
Clostridium butyricum
additional information
stimulating effect of amino compounds may be caused by disappearance of the inhibitory product of the reaction, pyridoxal 5'-phosphate, forming the corresponding Schiff base
Clostridium butyricum
Inhibitors
Inhibitors
Commentary
Organism
Structure
D-glutamate
slight inhibition
Clostridium butyricum
diisopropyl phosphofluoride
0.3 mM, complete inhibition
Clostridium butyricum
L-cysteine
about 35% inhibition
Clostridium butyricum
additional information
not inhibited by sulfhydryl compounds, e.g. PCMB, phenylmercuric acetate and mercuric chloride
Clostridium butyricum
pyridoxal 5'-phosphate
product inhibition
Clostridium butyricum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.004
-
pyridoxamine 5'-phosphate
pH 8.0, 37°C
Clostridium butyricum
0.01
-
pyridoxal 5'-phosphate
pH 8.0, 37°C
Clostridium butyricum
0.015
-
2-oxoglutarate
cosubstrate pyridoxamine 5'-phosphate, pH 8.0, 37°C
Clostridium butyricum
9
-
D-glutamate
pH 8.0, 37°C
Clostridium butyricum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
metal ions do not accelerate the reaction
Clostridium butyricum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium butyricum
-
IFO 3353
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, protamine treatment, DEAE-cellulose, hydroxylapatite, DEAE-Sephadex, Sephadex G-200, partial purification
Clostridium butyricum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.281
-
-
Clostridium butyricum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-aminobutanoate + 2-oxoglutarate
about 8% of activity compared to pyridoxamine 5'-phosphate
637633
Clostridium butyricum
4-oxobutanoate + D-glutamate
-
637633
Clostridium butyricum
?
pyridoxamine + 2-oxoglutarate
about 25% of activity compared to pyridoxamine 5'-phosphate
637633
Clostridium butyricum
pyridoxal + D-glutamate
-
637633
Clostridium butyricum
?
pyridoxamine 5'-phosphate + 2-oxoglutarate
-
637633
Clostridium butyricum
pyridoxal 5'-phosphate + D-glutamate
-
637633
Clostridium butyricum
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Clostridium butyricum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Clostridium butyricum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
4-aminobutyrate
stimulation, 177% of non-stimulated activity
Clostridium butyricum
D-asparagine
stimulation, 112% of non-stimulated activity
Clostridium butyricum
D-cysteine
stimulation, 135% of non-stimulated activity
Clostridium butyricum
D-methionine
stimulation, 127% of non-stimulated activity
Clostridium butyricum
DL-Ornithine
stimulation, 112% of non-stimulated activity
Clostridium butyricum
glycine
stimulation, 137% of non-stimulated activity
Clostridium butyricum
L-asparagine
stimulation, 112% of non-stimulated activity
Clostridium butyricum
L-glutamate
stimulation, 166% of non-stimulated activity
Clostridium butyricum
L-lysine
stimulation, 105% of non-stimulated activity
Clostridium butyricum
L-methionine
stimulation, 114% of non-stimulated activity
Clostridium butyricum
L-ornithine
stimulation, 112% of non-stimulated activity
Clostridium butyricum
additional information
stimulating effect of amino compounds may be caused by disappearance of the inhibitory product of the reaction, pyridoxal 5'-phosphate, forming the corresponding Schiff base
Clostridium butyricum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-glutamate
slight inhibition
Clostridium butyricum
diisopropyl phosphofluoride
0.3 mM, complete inhibition
Clostridium butyricum
L-cysteine
about 35% inhibition
Clostridium butyricum
additional information
not inhibited by sulfhydryl compounds, e.g. PCMB, phenylmercuric acetate and mercuric chloride
Clostridium butyricum
pyridoxal 5'-phosphate
product inhibition
Clostridium butyricum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.004
-
pyridoxamine 5'-phosphate
pH 8.0, 37°C
Clostridium butyricum
0.01
-
pyridoxal 5'-phosphate
pH 8.0, 37°C
Clostridium butyricum
0.015
-
2-oxoglutarate
cosubstrate pyridoxamine 5'-phosphate, pH 8.0, 37°C
Clostridium butyricum
9
-
D-glutamate
pH 8.0, 37°C
Clostridium butyricum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
metal ions do not accelerate the reaction
Clostridium butyricum
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, protamine treatment, DEAE-cellulose, hydroxylapatite, DEAE-Sephadex, Sephadex G-200, partial purification
Clostridium butyricum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.281
-
-
Clostridium butyricum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-aminobutanoate + 2-oxoglutarate
about 8% of activity compared to pyridoxamine 5'-phosphate
637633
Clostridium butyricum
4-oxobutanoate + D-glutamate
-
637633
Clostridium butyricum
?
pyridoxamine + 2-oxoglutarate
about 25% of activity compared to pyridoxamine 5'-phosphate
637633
Clostridium butyricum
pyridoxal + D-glutamate
-
637633
Clostridium butyricum
?
pyridoxamine 5'-phosphate + 2-oxoglutarate
-
637633
Clostridium butyricum
pyridoxal 5'-phosphate + D-glutamate
-
637633
Clostridium butyricum
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Clostridium butyricum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Clostridium butyricum
Other publictions for EC 2.6.1.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
637633
Tani
-
Studies on vitamin b6 metaboli ...
Clostridium butyricum
Agric. Biol. Chem.
36
181-188
1972
12
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5
4
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1
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-
-
1
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-
1
-
-
-
1
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3
-
1
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-
-
1
-
-
-
-
-
-
12
-
-
-
-
-
-
-
5
-
4
-
1
-
-
-
-
-
1
-
-
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
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