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Literature summary for 2.6.1.52 extracted from

  • Drewke, C., Klein, M.; Clade, D.; Arenz, A.; Muller, R.; Leistner, E.
    4-O-phosphoryl-L-threonine, a substrate of the pdxC(serC) gene product involved in vitamin B6 biosynthesis (1996), FEBS Lett., 390, 179-82.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
3-phosphonooxypyruvate MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C Escherichia coli
0.015
-
3-phosphonooxypyruvate PdxC(SerC) protein, pH 8.2, 37°C Escherichia coli
0.017
-
3-O-phospho-L-serine PdxC(SerC) protein, pH 8.2, 37°C Escherichia coli
0.037
-
3-O-phospho-L-serine MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C Escherichia coli
0.11
-
4-(phosphonooxy)-L-threonine MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C Escherichia coli
0.11
-
4-(phosphonooxy)-L-threonine PdxC(SerC) protein, pH 8.2, 37°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
PdxC(SerC) protein Escherichia coli
79000
-
MalE/PdxC(SerC) fusion protein, Coomassie blue stained SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
MalE/PdxC(SerC) fusion protein, affinity chromatography on an amylose resin, hydrolyzed in the presence of protease factor Xa Escherichia coli

Storage Stability

Storage Stability Organism
Whereas the fusion protein is stable over several weeks, the cleaved protein shows a clear loss of activity within 2 weeks at 4°C. Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-3-hydroxy-4-phosphobutanoate + L-glutamate 2-oxo-3-hydroxy-4-phosphobutanoate = 3-hydroxy-4-O-phosphoryl-2-oxobutyrate Escherichia coli 4-(phosphonooxy)-L-threonine + 2-oxoglutarate 4-phospho-hydroxy-L-threonine = 4-O-phosphoryl-L-threonine ?
3-O-phospho-L-serine + 2-oxoglutarate
-
Escherichia coli ?
-
?
3-phosphonooxypyruvate + 2-oxoglutarate
-
Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
3-O-phospho-L-serine:2-oxoglutarate aminotransferase
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
4-(phosphonooxy)-L-threonine MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C Escherichia coli
0.15
-
4-(phosphonooxy)-L-threonine PdxC(SerC) protein, pH 8.2, 37°C Escherichia coli
0.39
-
3-O-phospho-L-serine PdxC(SerC) protein, pH 8.2, 37°C Escherichia coli
0.63
-
3-O-phospho-L-serine MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C Escherichia coli
1.33
-
3-phosphonooxypyruvate MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C Escherichia coli
1.75
-
3-phosphonooxypyruvate PdxC(SerC) protein, pH 8.2, 37°C Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7.8 8.8
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Escherichia coli