KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
3-phosphonooxypyruvate | MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C | Escherichia coli | |
0.015 | - |
3-phosphonooxypyruvate | PdxC(SerC) protein, pH 8.2, 37°C | Escherichia coli | |
0.017 | - |
3-O-phospho-L-serine | PdxC(SerC) protein, pH 8.2, 37°C | Escherichia coli | |
0.037 | - |
3-O-phospho-L-serine | MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C | Escherichia coli | |
0.11 | - |
4-(phosphonooxy)-L-threonine | MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C | Escherichia coli | |
0.11 | - |
4-(phosphonooxy)-L-threonine | PdxC(SerC) protein, pH 8.2, 37°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
PdxC(SerC) protein | Escherichia coli |
79000 | - |
MalE/PdxC(SerC) fusion protein, Coomassie blue stained SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
MalE/PdxC(SerC) fusion protein, affinity chromatography on an amylose resin, hydrolyzed in the presence of protease factor Xa | Escherichia coli |
Storage Stability | Organism |
---|---|
Whereas the fusion protein is stable over several weeks, the cleaved protein shows a clear loss of activity within 2 weeks at 4°C. | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-hydroxy-4-phosphobutanoate + L-glutamate | 2-oxo-3-hydroxy-4-phosphobutanoate = 3-hydroxy-4-O-phosphoryl-2-oxobutyrate | Escherichia coli | 4-(phosphonooxy)-L-threonine + 2-oxoglutarate | 4-phospho-hydroxy-L-threonine = 4-O-phosphoryl-L-threonine | ? | |
3-O-phospho-L-serine + 2-oxoglutarate | - |
Escherichia coli | ? | - |
? | |
3-phosphonooxypyruvate + 2-oxoglutarate | - |
Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-O-phospho-L-serine:2-oxoglutarate aminotransferase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
4-(phosphonooxy)-L-threonine | MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C | Escherichia coli | |
0.15 | - |
4-(phosphonooxy)-L-threonine | PdxC(SerC) protein, pH 8.2, 37°C | Escherichia coli | |
0.39 | - |
3-O-phospho-L-serine | PdxC(SerC) protein, pH 8.2, 37°C | Escherichia coli | |
0.63 | - |
3-O-phospho-L-serine | MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C | Escherichia coli | |
1.33 | - |
3-phosphonooxypyruvate | MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C | Escherichia coli | |
1.75 | - |
3-phosphonooxypyruvate | PdxC(SerC) protein, pH 8.2, 37°C | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.8 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |