BRENDA - Enzyme Database show
show all sequences of 2.6.1.50

The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterization of an aminotransferase involved in the formation of 2-deoxystreptamine

Huang, F.; Haydock, S.F.; Mironenko, T.; Spiteller, D.; Li, Y.; Spencer, J.B.; Org. Biomol. Chem. 3, 1410-1418 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Streptomyces fradiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
x * 46951, His6-tagged protein, claculated, x * 45000, His6-tagged protein, SDS-PAGE
Streptomyces fradiae
46951
-
x * 46951, His6-tagged protein, claculated, x * 45000, His6-tagged protein, SDS-PAGE
Streptomyces fradiae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces fradiae
Q53U20
overexpression in Escherichia coli
-
Streptomyces fradiae NCIMB 8233
Q53U20
overexpression in Escherichia coli
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxy-scyllo-inosose + L-glutamine
-
663004
Streptomyces fradiae
2-deoxy-scyllo-inosamine + 2-ketoglutaramate
-
-
-
?
2-deoxy-scyllo-inosose + L-glutamine
-
663004
Streptomyces fradiae NCIMB 8233
2-deoxy-scyllo-inosamine + 2-ketoglutaramate
-
-
-
?
2-deoxystreptamine + pyruvate
-
663004
Streptomyces fradiae
2-keto-inosose + L-alanine
-
-
-
r
2-deoxystreptamine + pyruvate
-
663004
Streptomyces fradiae NCIMB 8233
2-keto-inosose + L-alanine
-
-
-
r
Subunits
Subunits
Commentary
Organism
?
x * 46951, His6-tagged protein, claculated, x * 45000, His6-tagged protein, SDS-PAGE
Streptomyces fradiae
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Streptomyces fradiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
x * 46951, His6-tagged protein, claculated, x * 45000, His6-tagged protein, SDS-PAGE
Streptomyces fradiae
46951
-
x * 46951, His6-tagged protein, claculated, x * 45000, His6-tagged protein, SDS-PAGE
Streptomyces fradiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxy-scyllo-inosose + L-glutamine
-
663004
Streptomyces fradiae
2-deoxy-scyllo-inosamine + 2-ketoglutaramate
-
-
-
?
2-deoxy-scyllo-inosose + L-glutamine
-
663004
Streptomyces fradiae NCIMB 8233
2-deoxy-scyllo-inosamine + 2-ketoglutaramate
-
-
-
?
2-deoxystreptamine + pyruvate
-
663004
Streptomyces fradiae
2-keto-inosose + L-alanine
-
-
-
r
2-deoxystreptamine + pyruvate
-
663004
Streptomyces fradiae NCIMB 8233
2-keto-inosose + L-alanine
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 46951, His6-tagged protein, claculated, x * 45000, His6-tagged protein, SDS-PAGE
Streptomyces fradiae
Other publictions for EC 2.6.1.50
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
688331
Kurumbang
Heterologous production and de ...
Streptomyces fradiae
J. Microbiol. Biotechnol.
18
866-873
2008
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-
1
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-
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-
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1
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1
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1
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1
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-
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-
661949
Yokoyama
Stereochemical recognition of ...
Bacillus circulans, Bacillus circulans SANK 72073
J. Am. Chem. Soc.
127
5869-5874
2005
-
-
-
-
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-
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2
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2
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1
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4
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1
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1
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2
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4
-
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-
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663004
Huang
The neomycin biosynthetic gene ...
Streptomyces fradiae, Streptomyces fradiae NCIMB 8233
Org. Biomol. Chem.
3
1410-1418
2005
-
-
1
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
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-
4
1
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-
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1
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2
-
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-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672584
Kharel
Characterization of L-glutamin ...
Streptoalloteichus tenebrarius
Bioorg. Med. Chem. Lett.
15
89-92
2005
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-
1
-
-
-
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1
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1
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2
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1
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1
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2
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-
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-
-
-
-
-
-
661375
Kharel
An approach for cloning biosyn ...
Streptoalloteichus tenebrarius
Biotechnol. Lett.
25
2041-2047
2003
-
-
-
-
-
-
-
-
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1
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2
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2
-
-
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-
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-
640131
Tamegai
First identification of Strept ...
Bacillus circulans, Streptoalloteichus hindustanus, Streptomyces fradiae, Streptomyces ribosidificus
J. Antibiot.
55
1016-1018
2002
-
1
4
-
-
-
-
-
-
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4
-
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1
4
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640130
Ahlert
Identification of stsC, the ge ...
no activity in Escherichia coli, no activity in Streptomyces lividans, Streptomyces bikiniensis, Streptomyces bikiniensis NRRL 1049b, Streptomyces griseus, Streptomyces hygroscopicus
Arch. Microbiol.
168
102-113
1997
-
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1
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-
-
-
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-
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3
4
-
6
-
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1
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1
6
1
-
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-
-
-
-
-
-
-
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-
1
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-
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3
4
-
-
-
1
-
-
-
1
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640129
Walker
Enzymic synthesis of aminocycl ...
Streptomyces griseocarneus, Streptomyces hygroscopicus, Streptomyces netropsis
J. Bacteriol.
177
818-822
1995
-
2
-
-
-
-
-
-
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3
-
5
-
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4
-
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10
-
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2
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3
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4
-
-
10
-
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-
-
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-
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-
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-
-
-
-
640128
Lucher
Reactions catalyzed by purifie ...
Micromonospora echinospora
Antimicrob. Agents Chemother.
33
452-459
1989
-
1
-
-
-
1
2
-
-
-
1
1
-
1
-
-
1
-
-
1
1
2
21
-
-
-
1
-
1
1
-
1
-
-
-
-
1
-
1
-
-
1
-
2
-
-
-
-
1
1
-
-
-
1
-
1
1
2
21
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
640127
Walker
L-glutamine:keto-scyllo-inosit ...
Streptomyces bikiniensis, Streptomyces griseus, Streptomyces hygroscopicus, Streptomyces ornatus
Methods Enzymol.
43
439-443
1975
-
-
-
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1
-
-
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-
-
4
-
4
-
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-
-
4
-
-
7
-
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-
1
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-
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4
-
-
-
-
-
-
4
-
-
1
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4
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4
-
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7
-
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1
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-