BRENDA - Enzyme Database show
show all sequences of 2.6.1.50

Reactions catalyzed by purified L-glutamine:keto-scyllo-inositol aminotransferase, an enzyme required for biosynthesis of aminocyclitol antibiotics

Lucher, L.A.; Chen, Y.M.; Walker, J.B.; Antimicrob. Agents Chemother. 33, 452-459 (1989)

Data extracted from this reference:

Application
Application
Commentary
Organism
pharmacology
2-deoxystreptamine is a component of numerous clinically important antibiotics such as gentamicin, neomycin, tobramycin, amikacin and hygromycin
Micromonospora echinospora
General Stability
General Stability
Organism
activity rapidly lost when dialyzed
Micromonospora echinospora
Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithio-bis(2-nitrobenzoic acid)
reduces specific activity by 50%
Micromonospora echinospora
p-hydroxymercuribenzoate
reduces specific activity by 50%
Micromonospora echinospora
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64000
76000
gel filtration
Micromonospora echinospora
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone
Micromonospora echinospora
2-deoxystreptamine biosynthesis
2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Micromonospora echinospora
-
ATCC 15835
-
Purification (Commentary)
Commentary
Organism
-
Micromonospora echinospora
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Micromonospora echinospora
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.24
-
-
Micromonospora echinospora
Storage Stability
Storage Stability
Organism
-20°C, stored at concentrations greater than 1mg/ml, stable for several months with little loss of activity
Micromonospora echinospora
4°C, inactivated within 1 month
Micromonospora echinospora
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxystreptamine + 2-ketoglutaramate
-
640128
Micromonospora echinospora
2-deoxy-keto-inosamine + L-glutamine
-
640128
Micromonospora echinospora
r
2-deoxystreptamine + 2-oxoglutaramate
-
640128
Micromonospora echinospora
2-deoxy-scyllo-inositol + L-glutamine
-
-
-
r
2-deoxystreptamine + keto-scyllo-inositol
-
640128
Micromonospora echinospora
2-deoxy-scyllo-inositol + amino-deoxy-keto-scyllo-inositol
-
-
-
r
aminodeoxy-scyllo-inositol + 2-oxoglutaramate
-
640128
Micromonospora echinospora
scyllo-inositol + L-glutamine
-
-
-
r
aminodeoxy-scyllo-inositol + keto-scyllo-inositol
-
640128
Micromonospora echinospora
amino-scyllo-inositol + aminodeoxy-keto-scyllo-inositol
-
-
-
r
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone
keto-scyllo-inositol
640128
Micromonospora echinospora
2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
-
-
r
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone
2-deoxystreptamine biosynthesis
640128
Micromonospora echinospora
2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
-
-
r
L-glutamine + 2-oxoadipate
-
640128
Micromonospora echinospora
2-oxoglutaramate + L-2-amino-1,6-hexandioate
-
-
-
r
L-glutamine + 2-oxobutyrate
-
640128
Micromonospora echinospora
2-oxoglutaramate + 2-aminobutyrate
-
-
-
r
L-glutamine + 2-oxocaproate
-
640128
Micromonospora echinospora
2-oxoglutaramate + L-norleucine
-
-
-
r
L-glutamine + 2-oxoglutaramate
-
640128
Micromonospora echinospora
2-oxoglutaramate + L-glutamine
-
-
-
r
L-glutamine + 2-oxoglutarate
-
640128
Micromonospora echinospora
2-oxoglutaramate + L-glutamate
-
-
-
r
L-glutamine + 2-oxovalerate
-
640128
Micromonospora echinospora
2-oxoglutaramate + L-norvaline
-
-
-
r
L-glutamine + DL-epi-inosose
-
640128
Micromonospora echinospora
2-oxoglutaramate + amino-deoxy-inosose
-
-
-
r
L-glutamine + glyoxalate
-
640128
Micromonospora echinospora
2-oxoglutaramate + glycine
-
-
-
r
L-glutamine + oxaloacetate
-
640128
Micromonospora echinospora
2-oxoglutaramate + L-aspartate
-
-
-
r
L-glutamine + pyruvate
-
640128
Micromonospora echinospora
2-oxoglutaramate + L-alanine
-
-
-
r
N3-methyl-2-deoxy-streptamine + keto-scyllo-inositol
-
640128
Micromonospora echinospora
3-methylamino-2-deoxy-scyllo-inositol + 1-amino-1-deoxy-scyllo-inositol
-
-
-
r
N3-methyl-2-deoxystreptamine + 2-oxoglutaramate
-
640128
Micromonospora echinospora
3-methylamino-2-deoxy-scyllo-inositol + L-glutamine
-
-
-
r
streptamine + 2-oxoglutaramate
-
640128
Micromonospora echinospora
amino-scyllo-inositol + L-glutamine
-
-
-
r
streptamine + keto-scyllo-inositol
-
640128
Micromonospora echinospora
amino-scyllo-inositol + amino-deoxy-keto-scyllo-inositol
-
-
-
r
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
heat labile, loses all activity upon incubation for 20 min
Micromonospora echinospora
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Micromonospora echinospora
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
9
-
Micromonospora echinospora
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Micromonospora echinospora
Application (protein specific)
Application
Commentary
Organism
pharmacology
2-deoxystreptamine is a component of numerous clinically important antibiotics such as gentamicin, neomycin, tobramycin, amikacin and hygromycin
Micromonospora echinospora
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Micromonospora echinospora
General Stability (protein specific)
General Stability
Organism
activity rapidly lost when dialyzed
Micromonospora echinospora
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithio-bis(2-nitrobenzoic acid)
reduces specific activity by 50%
Micromonospora echinospora
p-hydroxymercuribenzoate
reduces specific activity by 50%
Micromonospora echinospora
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64000
76000
gel filtration
Micromonospora echinospora
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone
Micromonospora echinospora
2-deoxystreptamine biosynthesis
2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Micromonospora echinospora
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Micromonospora echinospora
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.24
-
-
Micromonospora echinospora
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stored at concentrations greater than 1mg/ml, stable for several months with little loss of activity
Micromonospora echinospora
4°C, inactivated within 1 month
Micromonospora echinospora
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-deoxystreptamine + 2-ketoglutaramate
-
640128
Micromonospora echinospora
2-deoxy-keto-inosamine + L-glutamine
-
640128
Micromonospora echinospora
r
2-deoxystreptamine + 2-oxoglutaramate
-
640128
Micromonospora echinospora
2-deoxy-scyllo-inositol + L-glutamine
-
-
-
r
2-deoxystreptamine + keto-scyllo-inositol
-
640128
Micromonospora echinospora
2-deoxy-scyllo-inositol + amino-deoxy-keto-scyllo-inositol
-
-
-
r
aminodeoxy-scyllo-inositol + 2-oxoglutaramate
-
640128
Micromonospora echinospora
scyllo-inositol + L-glutamine
-
-
-
r
aminodeoxy-scyllo-inositol + keto-scyllo-inositol
-
640128
Micromonospora echinospora
amino-scyllo-inositol + aminodeoxy-keto-scyllo-inositol
-
-
-
r
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone
keto-scyllo-inositol
640128
Micromonospora echinospora
2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
-
-
r
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone
2-deoxystreptamine biosynthesis
640128
Micromonospora echinospora
2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
-
-
-
r
L-glutamine + 2-oxoadipate
-
640128
Micromonospora echinospora
2-oxoglutaramate + L-2-amino-1,6-hexandioate
-
-
-
r
L-glutamine + 2-oxobutyrate
-
640128
Micromonospora echinospora
2-oxoglutaramate + 2-aminobutyrate
-
-
-
r
L-glutamine + 2-oxocaproate
-
640128
Micromonospora echinospora
2-oxoglutaramate + L-norleucine
-
-
-
r
L-glutamine + 2-oxoglutaramate
-
640128
Micromonospora echinospora
2-oxoglutaramate + L-glutamine
-
-
-
r
L-glutamine + 2-oxoglutarate
-
640128
Micromonospora echinospora
2-oxoglutaramate + L-glutamate
-
-
-
r
L-glutamine + 2-oxovalerate
-
640128
Micromonospora echinospora
2-oxoglutaramate + L-norvaline
-
-
-
r
L-glutamine + DL-epi-inosose
-
640128
Micromonospora echinospora
2-oxoglutaramate + amino-deoxy-inosose
-
-
-
r
L-glutamine + glyoxalate
-
640128
Micromonospora echinospora
2-oxoglutaramate + glycine
-
-
-
r
L-glutamine + oxaloacetate
-
640128
Micromonospora echinospora
2-oxoglutaramate + L-aspartate
-
-
-
r
L-glutamine + pyruvate
-
640128
Micromonospora echinospora
2-oxoglutaramate + L-alanine
-
-
-
r
N3-methyl-2-deoxy-streptamine + keto-scyllo-inositol
-
640128
Micromonospora echinospora
3-methylamino-2-deoxy-scyllo-inositol + 1-amino-1-deoxy-scyllo-inositol
-
-
-
r
N3-methyl-2-deoxystreptamine + 2-oxoglutaramate
-
640128
Micromonospora echinospora
3-methylamino-2-deoxy-scyllo-inositol + L-glutamine
-
-
-
r
streptamine + 2-oxoglutaramate
-
640128
Micromonospora echinospora
amino-scyllo-inositol + L-glutamine
-
-
-
r
streptamine + keto-scyllo-inositol
-
640128
Micromonospora echinospora
amino-scyllo-inositol + amino-deoxy-keto-scyllo-inositol
-
-
-
r
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
heat labile, loses all activity upon incubation for 20 min
Micromonospora echinospora
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Micromonospora echinospora
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
9
-
Micromonospora echinospora
Other publictions for EC 2.6.1.50
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
688331
Kurumbang
Heterologous production and de ...
Streptomyces fradiae
J. Microbiol. Biotechnol.
18
866-873
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661949
Yokoyama
Stereochemical recognition of ...
Bacillus circulans, Bacillus circulans SANK 72073
J. Am. Chem. Soc.
127
5869-5874
2005
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663004
Huang
The neomycin biosynthetic gene ...
Streptomyces fradiae, Streptomyces fradiae NCIMB 8233
Org. Biomol. Chem.
3
1410-1418
2005
-
-
1
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672584
Kharel
Characterization of L-glutamin ...
Streptoalloteichus tenebrarius
Bioorg. Med. Chem. Lett.
15
89-92
2005
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661375
Kharel
An approach for cloning biosyn ...
Streptoalloteichus tenebrarius
Biotechnol. Lett.
25
2041-2047
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640131
Tamegai
First identification of Strept ...
Bacillus circulans, Streptoalloteichus hindustanus, Streptomyces fradiae, Streptomyces ribosidificus
J. Antibiot.
55
1016-1018
2002
-
1
4
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640130
Ahlert
Identification of stsC, the ge ...
no activity in Escherichia coli, no activity in Streptomyces lividans, Streptomyces bikiniensis, Streptomyces bikiniensis NRRL 1049b, Streptomyces griseus, Streptomyces hygroscopicus
Arch. Microbiol.
168
102-113
1997
-
-
1
-
-
-
-
-
-
-
3
4
-
6
-
-
1
-
-
-
-
1
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
4
-
-
-
1
-
-
-
1
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640129
Walker
Enzymic synthesis of aminocycl ...
Streptomyces griseocarneus, Streptomyces hygroscopicus, Streptomyces netropsis
J. Bacteriol.
177
818-822
1995
-
2
-
-
-
-
-
-
-
-
-
3
-
5
-
-
-
-
-
4
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
4
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640128
Lucher
Reactions catalyzed by purifie ...
Micromonospora echinospora
Antimicrob. Agents Chemother.
33
452-459
1989
-
1
-
-
-
1
2
-
-
-
1
1
-
1
-
-
1
-
-
1
1
2
21
-
-
-
1
-
1
1
-
1
-
-
-
-
1
-
1
-
-
1
-
2
-
-
-
-
1
1
-
-
-
1
-
1
1
2
21
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
640127
Walker
L-glutamine:keto-scyllo-inosit ...
Streptomyces bikiniensis, Streptomyces griseus, Streptomyces hygroscopicus, Streptomyces ornatus
Methods Enzymol.
43
439-443
1975
-
-
-
-
-
1
-
-
-
-
-
4
-
4
-
-
-
-
-
4
-
-
7
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
7
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-