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Literature summary for 2.6.1.5 extracted from

  • Wang, M.; Toda, K.; Maeda, H.A.
    Biochemical properties and subcellular localization of tyrosine aminotransferases in Arabidopsis thaliana (2016), Phytochemistry, 132, 16-25 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.204
-
L-tyrosine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.41
-
4-hydroxyphenylpyruvate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.455
-
phenylpyruvate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.74
-
4-methylthio-2-oxobutanoate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.807
-
4-methylthio-2-oxobutanoate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
1.28
-
phenylpyruvate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
1.3
-
4-methylthio-2-oxobutanoate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
1.7
-
L-methionine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.9
-
L-tyrosine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
3.06
-
phenylpyruvate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
3.33
-
2-oxoglutarate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
3.43
-
phenylpyruvate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
3.46
-
4-methylthio-2-oxobutanoate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
6.7
-
L-phenylalanine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
6.94
-
L-phenylalanine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
7.62
-
2-oxoglutarate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
10.1
-
4-hydroxyphenylpyruvate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
24.2
-
L-methionine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
42.9
-
oxaloacetate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Arabidopsis thaliana 5829
-
plastid
-
Arabidopsis thaliana 9536
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q67Y55
-
-
Arabidopsis thaliana Q9FN30
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Sephadex G50 gel filtration Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylpyruvate + L-glutamate
-
Arabidopsis thaliana L-tyrosine + 2-oxoglutarate
-
r
4-hydroxyphenylpyruvate + L-glutamate favoured direction of reaction for isoform TAT2 Arabidopsis thaliana L-tyrosine + 2-oxoglutarate
-
r
4-methylthio-2-oxobutanoate + L-glutamate
-
Arabidopsis thaliana ?
-
r
L-alanine + 2-oxoglutarate
-
Arabidopsis thaliana pyruvate + L-glutamate
-
r
L-arginine + 2-oxoglutarate
-
Arabidopsis thaliana ?
-
r
L-asparagine + 2-oxoglutarate
-
Arabidopsis thaliana 2-oxosuccinamate + L-glutamate
-
r
L-cysteine + 2-oxoglutarate
-
Arabidopsis thaliana 3-mercapto-2-oxopropanoate + L-glutamate
-
r
L-glutamine + 2-oxoglutarate
-
Arabidopsis thaliana 5-amino-2,5-dioxopentanoic acid + L-glutamate
-
r
L-histidine + 2-oxoglutarate
-
Arabidopsis thaliana 3-(1H-imidazol-4-yl)-2-oxopropanoic acid + L-glutamate
-
r
L-leucine + 2-oxoglutarate
-
Arabidopsis thaliana 4-methyl-2-oxopentanoic acid + L-glutamate
-
r
L-methionine + 2-oxoglutarate
-
Arabidopsis thaliana 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
r
L-phenylalanine + 2-oxoglutarate
-
Arabidopsis thaliana phenylpyruvate + L-glutamate
-
r
L-serine + 2-oxoglutarate
-
Arabidopsis thaliana 3-hydroxy-2-oxopropanoic acid + L-glutamate
-
r
L-tryptophan + 2-oxoglutarate
-
Arabidopsis thaliana 3-(1H-indol-3-yl)-2-oxopropanoate + L-glutamate
-
r
L-tyrosine + 2-oxoglutarate
-
Arabidopsis thaliana 4-hydroxyphenylpyruvate + L-glutamate
-
r
L-tyrosine + 2-oxoglutarate best substrate and favoured direction of reaction for isoform TAT1 Arabidopsis thaliana 4-hydroxyphenylpyruvate + L-glutamate
-
r
L-tyrosine + 4-methylthio-2-oxobutanoate
-
Arabidopsis thaliana ?
-
r
L-tyrosine + oxaloacetate
-
Arabidopsis thaliana 4-hydroxyphenylpyruvate + L-aspartate
-
r
L-tyrosine + phenylpyruvate
-
Arabidopsis thaliana 3-(4-hydroxyphenyl)-2-oxopropanoate + L-phenylalanine
-
r
additional information isoform TAT1 shows no activity with oxaloacetate, L-Ala, L-Ser, L-Cys, L-Asp, L-Asn, L-Gln, and L-Arg Arabidopsis thaliana ?
-
-
oxaloacetate + L-glutamate
-
Arabidopsis thaliana L-aspartate + 2-oxoglutarate
-
r
phenylpyruvate + L-glutamate
-
Arabidopsis thaliana 2-oxoglutarate + L-phenylalanine
-
r

Synonyms

Synonyms Comment Organism
TAT
-
Arabidopsis thaliana
TAT1 isoform Arabidopsis thaliana
TAT2 isoform Arabidopsis thaliana
Tyr aminotransferase
-
Arabidopsis thaliana
tyrosine aminotransferase
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
phenylpyruvate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.37
-
4-methylthio-2-oxobutanoate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.43
-
4-hydroxyphenylpyruvate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.47
-
phenylpyruvate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.51
-
L-phenylalanine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.53
-
L-methionine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.53
-
2-oxoglutarate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.56
-
oxaloacetate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.7
-
4-methylthio-2-oxobutanoate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.75
-
L-tyrosine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
18.5
-
4-methylthio-2-oxobutanoate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
20.1
-
4-methylthio-2-oxobutanoate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
23.9
-
4-hydroxyphenylpyruvate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
24.5
-
phenylpyruvate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
26.6
-
L-methionine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
30.2
-
phenylpyruvate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
31.6
-
L-phenylalanine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
48
-
2-oxoglutarate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
57.3
-
L-tyrosine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.02
-
oxaloacetate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.04
-
phenylpyruvate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.07
-
2-oxoglutarate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.08
-
L-phenylalanine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.23
-
L-tyrosine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.32
-
L-methionine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.49
-
phenylpyruvate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
0.51
-
4-methylthio-2-oxobutanoate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
1.1
-
L-methionine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
1.15
-
4-methylthio-2-oxobutanoate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
1.38
-
4-hydroxyphenylpyruvate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.5
-
4-hydroxyphenylpyruvate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
4.5
-
L-phenylalanine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
5.5
-
4-methylthio-2-oxobutanoate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
9.9
-
phenylpyruvate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
15.1
-
2-oxoglutarate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
15.5
-
4-methylthio-2-oxobutanoate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
54.2
-
phenylpyruvate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
281.9
-
L-tyrosine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana