Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.204 | - |
L-tyrosine | isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.41 | - |
4-hydroxyphenylpyruvate | isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.455 | - |
phenylpyruvate | isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.74 | - |
4-methylthio-2-oxobutanoate | isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.807 | - |
4-methylthio-2-oxobutanoate | isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
1.28 | - |
phenylpyruvate | isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
1.3 | - |
4-methylthio-2-oxobutanoate | isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
1.7 | - |
L-methionine | isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
2.9 | - |
L-tyrosine | isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
3.06 | - |
phenylpyruvate | isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
3.33 | - |
2-oxoglutarate | isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
3.43 | - |
phenylpyruvate | isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
3.46 | - |
4-methylthio-2-oxobutanoate | isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
6.7 | - |
L-phenylalanine | isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
6.94 | - |
L-phenylalanine | isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
7.62 | - |
2-oxoglutarate | isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
10.1 | - |
4-hydroxyphenylpyruvate | isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
24.2 | - |
L-methionine | isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
42.9 | - |
oxaloacetate | isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis thaliana | 5829 | - |
plastid | - |
Arabidopsis thaliana | 9536 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q67Y55 | - |
- |
Arabidopsis thaliana | Q9FN30 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Sephadex G50 gel filtration | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + L-glutamate | - |
Arabidopsis thaliana | L-tyrosine + 2-oxoglutarate | - |
r | |
4-hydroxyphenylpyruvate + L-glutamate | favoured direction of reaction for isoform TAT2 | Arabidopsis thaliana | L-tyrosine + 2-oxoglutarate | - |
r | |
4-methylthio-2-oxobutanoate + L-glutamate | - |
Arabidopsis thaliana | ? | - |
r | |
L-alanine + 2-oxoglutarate | - |
Arabidopsis thaliana | pyruvate + L-glutamate | - |
r | |
L-arginine + 2-oxoglutarate | - |
Arabidopsis thaliana | ? | - |
r | |
L-asparagine + 2-oxoglutarate | - |
Arabidopsis thaliana | 2-oxosuccinamate + L-glutamate | - |
r | |
L-cysteine + 2-oxoglutarate | - |
Arabidopsis thaliana | 3-mercapto-2-oxopropanoate + L-glutamate | - |
r | |
L-glutamine + 2-oxoglutarate | - |
Arabidopsis thaliana | 5-amino-2,5-dioxopentanoic acid + L-glutamate | - |
r | |
L-histidine + 2-oxoglutarate | - |
Arabidopsis thaliana | 3-(1H-imidazol-4-yl)-2-oxopropanoic acid + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Arabidopsis thaliana | 4-methyl-2-oxopentanoic acid + L-glutamate | - |
r | |
L-methionine + 2-oxoglutarate | - |
Arabidopsis thaliana | 4-methylsulfanyl-2-oxobutanoate + L-glutamate | - |
r | |
L-phenylalanine + 2-oxoglutarate | - |
Arabidopsis thaliana | phenylpyruvate + L-glutamate | - |
r | |
L-serine + 2-oxoglutarate | - |
Arabidopsis thaliana | 3-hydroxy-2-oxopropanoic acid + L-glutamate | - |
r | |
L-tryptophan + 2-oxoglutarate | - |
Arabidopsis thaliana | 3-(1H-indol-3-yl)-2-oxopropanoate + L-glutamate | - |
r | |
L-tyrosine + 2-oxoglutarate | - |
Arabidopsis thaliana | 4-hydroxyphenylpyruvate + L-glutamate | - |
r | |
L-tyrosine + 2-oxoglutarate | best substrate and favoured direction of reaction for isoform TAT1 | Arabidopsis thaliana | 4-hydroxyphenylpyruvate + L-glutamate | - |
r | |
L-tyrosine + 4-methylthio-2-oxobutanoate | - |
Arabidopsis thaliana | ? | - |
r | |
L-tyrosine + oxaloacetate | - |
Arabidopsis thaliana | 4-hydroxyphenylpyruvate + L-aspartate | - |
r | |
L-tyrosine + phenylpyruvate | - |
Arabidopsis thaliana | 3-(4-hydroxyphenyl)-2-oxopropanoate + L-phenylalanine | - |
r | |
additional information | isoform TAT1 shows no activity with oxaloacetate, L-Ala, L-Ser, L-Cys, L-Asp, L-Asn, L-Gln, and L-Arg | Arabidopsis thaliana | ? | - |
- |
|
oxaloacetate + L-glutamate | - |
Arabidopsis thaliana | L-aspartate + 2-oxoglutarate | - |
r | |
phenylpyruvate + L-glutamate | - |
Arabidopsis thaliana | 2-oxoglutarate + L-phenylalanine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
TAT | - |
Arabidopsis thaliana |
TAT1 | isoform | Arabidopsis thaliana |
TAT2 | isoform | Arabidopsis thaliana |
Tyr aminotransferase | - |
Arabidopsis thaliana |
tyrosine aminotransferase | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
phenylpyruvate | isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.37 | - |
4-methylthio-2-oxobutanoate | isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.43 | - |
4-hydroxyphenylpyruvate | isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.47 | - |
phenylpyruvate | isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.51 | - |
L-phenylalanine | isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.53 | - |
L-methionine | isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.53 | - |
2-oxoglutarate | isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.56 | - |
oxaloacetate | isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.7 | - |
4-methylthio-2-oxobutanoate | isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.75 | - |
L-tyrosine | isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
18.5 | - |
4-methylthio-2-oxobutanoate | isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
20.1 | - |
4-methylthio-2-oxobutanoate | isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
23.9 | - |
4-hydroxyphenylpyruvate | isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
24.5 | - |
phenylpyruvate | isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
26.6 | - |
L-methionine | isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
30.2 | - |
phenylpyruvate | isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
31.6 | - |
L-phenylalanine | isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
48 | - |
2-oxoglutarate | isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
57.3 | - |
L-tyrosine | isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
oxaloacetate | isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.04 | - |
phenylpyruvate | isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.07 | - |
2-oxoglutarate | isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.08 | - |
L-phenylalanine | isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.23 | - |
L-tyrosine | isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.32 | - |
L-methionine | isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.49 | - |
phenylpyruvate | isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
0.51 | - |
4-methylthio-2-oxobutanoate | isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
1.1 | - |
L-methionine | isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
1.15 | - |
4-methylthio-2-oxobutanoate | isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
1.38 | - |
4-hydroxyphenylpyruvate | isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
2.5 | - |
4-hydroxyphenylpyruvate | isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
4.5 | - |
L-phenylalanine | isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
5.5 | - |
4-methylthio-2-oxobutanoate | isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
9.9 | - |
phenylpyruvate | isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
15.1 | - |
2-oxoglutarate | isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
15.5 | - |
4-methylthio-2-oxobutanoate | isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
54.2 | - |
phenylpyruvate | isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana | |
281.9 | - |
L-tyrosine | isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C | Arabidopsis thaliana |