Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.5 extracted from

  • Yoo, H.; Widhalm, J.; Qian, Y.; Maeda, H.; Cooper, B.; Jannasch, A.; Gonda, I.; Lewinsohn, E.; Rhodes, D.; Dudareva, N.
    An alternative pathway contributes to phenylalanine biosynthesis in plants via a cytosolic tyrosine:phenylpyruvate aminotransferase (2013), Nat. Commun., 4, 2833.
    View publication on PubMed
Search for more literature for 2.6.1.5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 phenylpyruvate cosubstrate L-tyrosine, pH 10.0, 30°C Petunia x hybrida
1.8
-
 2-oxoglutarate cosubstrate phenylalanine, pH 10.0, 30°C Petunia x hybrida
1.9
-
 L-tyrosine cosubstrate phenylpyruvate, pH 10.0, 30°C Petunia x hybrida
2.6
-
 phenylpyruvate cosubstrate L-glutamate, pH 10.0, 30°C Petunia x hybrida
4.3
-
 L-phenylalanine cosubstrate 4-hydroxyphenylpyruvate, pH 10.0, 30°C Petunia x hybrida
7.9
-
 4-hydroxyphenylpyruvate cosubstrate phenylalanine, pH 10.0, 30°C Petunia x hybrida
11.1
-
 L-glutamate cosubstrate phenylpyruvate, pH 10.0, 30°C Petunia x hybrida
13.8
-
 L-phenylalanine cosubstrate 2-oxoglutarate, pH 10.0, 30°C Petunia x hybrida

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Petunia x hybrida 5829
-

Organism

Organism UniProt Comment Textmining
Petunia x hybrida V5M241
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + L-phenylalanine
-
 Petunia x hybrida L-glutamate + phenylpyruvate
-
 ?
4-hydroxyphenylpyruvate + L-phenylalanine
-
 Petunia x hybrida L-glutamate + phenylpyruvate
-
 ?
L-glutamate + phenylpyruvate
-
 Petunia x hybrida 2-oxoglutarate + L-phenylalanine
-
 r
L-tyrosine + phenylpyruvate
-
 Petunia x hybrida 4-hydroxyphenylpyruvate + L-phenylalanine
-
 r
additional information activities with glutamate, methionine and leucine do not exceed 13% of the activity with tyrosine. Only phenylpyruvate and 2-oxoglutarate effectively serve as acceptors, and no activity is detected with prephenate Petunia x hybrida ?
-
 ?

Synonyms

Synonyms Comment Organism
PPY-AT
-
 Petunia x hybrida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.17
-
 L-phenylalanine cosubstrate 4-hydroxyphenylpyruvate, pH 10.0, 30°C Petunia x hybrida
0.44
-
 L-phenylalanine cosubstrate 2-oxoglutarate, pH 10.0, 30°C Petunia x hybrida
0.44
-
 L-glutamate cosubstrate phenylpyruvate, pH 10.0, 30°C Petunia x hybrida
0.7
-
 4-hydroxyphenylpyruvate cosubstrate phenylalanine, pH 10.0, 30°C Petunia x hybrida
0.92
-
 2-oxoglutarate cosubstrate phenylalanine, pH 10.0, 30°C Petunia x hybrida
1.19
-
 phenylpyruvate cosubstrate L-glutamate, pH 10.0, 30°C Petunia x hybrida
1.46
-
 phenylpyruvate cosubstrate L-tyrosine, pH 10.0, 30°C Petunia x hybrida
1.48
-
 L-tyrosine cosubstrate phenylpyruvate, pH 10.0, 30°C Petunia x hybrida

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Petunia x hybrida

General Information

General Information Comment Organism
physiological function Petunia hybrida utilizes a microbial-like phenylpyruvate pathway to produce phenylalanine, and flux through this route is increased when the entry point to the arogenate pathway is limiting. The plant phenylpyruvate pathway utilizes cytosolic tyrosine aminotransferase that links the coordinated catabolism of tyrosine to serve as the amino donor. The enzyme is able to functionally complement the Escherichia coli phenylalanine auxotrophic mutant DL39 which lacks the three aminotransferases, AspC (aspartate aminotransferase), TyrB (L-Tyrosine aminotransferase) and IlvE (branched-chain aminotransferase) Petunia x hybrida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.04
-
 L-phenylalanine cosubstrate 2-oxoglutarate, pH 10.0, 30°C Petunia x hybrida
0.04
-
 L-phenylalanine cosubstrate 4-hydroxyphenylpyruvate, pH 10.0, 30°C Petunia x hybrida
0.04
-
 L-glutamate cosubstrate phenylpyruvate, pH 10.0, 30°C Petunia x hybrida
0.09
-
 4-hydroxyphenylpyruvate cosubstrate phenylalanine, pH 10.0, 30°C Petunia x hybrida
0.46
-
 phenylpyruvate cosubstrate L-glutamate, pH 10.0, 30°C Petunia x hybrida
0.51
-
 2-oxoglutarate cosubstrate phenylalanine, pH 10.0, 30°C Petunia x hybrida
0.77
-
 L-tyrosine cosubstrate phenylpyruvate, pH 10.0, 30°C Petunia x hybrida
0.96
-
 phenylpyruvate cosubstrate L-tyrosine, pH 10.0, 30°C Petunia x hybrida