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Literature summary for 2.6.1.5 extracted from

  • Mehere, P.; Han, Q.; Lemkul, J.A.; Vavricka, C.J.; Robinson, H.; Bevan, D.R.; Li, J.
    Tyrosine aminotransferase: biochemical and structural properties and molecular dynamics simulations (2010), Protein Cell, 1, 1023-1032.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
through macromolecular crystallography the mTAT crystal structure is determined at 2.9 Å resolution. The crystal structure reveal the interaction between the pyridoxal-5'-phosphate cofactor and the enzyme, as well as the formation of a disulphide bond Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 4-hydroxyphenylpyruvate pH 7.0, 38°C Mus musculus
0.9
-
 phenylpyruvate pH 7.0, 38°C Mus musculus
1.8
-
 2-oxoglutarate pH 7.0, 38°C Mus musculus
1.8
-
 L-tyrosine pH 7.0, 38°C Mus musculus
4.9
-
 L-glutamate pH 7.0, 38°C Mus musculus
7.3
-
 2-Oxohexanoate pH 7.0, 38°C Mus musculus
11.4
-
 L-phenylalanine pH 7.0, 38°C Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using affinity, Source-Q and gel-filtration chromatography Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + 4-hydroxyphenylpyruvate
-
 Mus musculus L-tyrosine + 2-oxoglutarate
-
 ?
L-phenylalanine + 2-oxoglutarate
-
 Mus musculus 2-oxo-3-phenylpropanoate + L-glutamate
-
 ?
L-tyrosine + 2-oxoglutarate
-
 Mus musculus 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
 ?
L-tyrosine + 2-oxohexanoate
-
 Mus musculus 3-(4-hydroxyphenyl)-2-oxopropanoate + (2S)-2-aminohexanoate
-
 ?
L-tyrosine + phenylpyruvate
-
 Mus musculus 3-(4-hydroxyphenyl)-2-oxopropanoate + L-phenylalanine
-
 ?

Synonyms

Synonyms Comment Organism
mTAT
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
 assay at Mus musculus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 70 highest activity Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.83
-
 2-Oxohexanoate pH 7.0, 38°C Mus musculus
2.66
-
 phenylpyruvate pH 7.0, 38°C Mus musculus
9.33
-
 L-glutamate pH 7.0, 38°C Mus musculus
15.33
-
 L-phenylalanine pH 7.0, 38°C Mus musculus
15.83
-
 4-hydroxyphenylpyruvate pH 7.0, 38°C Mus musculus
19.33
-
 L-tyrosine pH 7.0, 38°C Mus musculus
25.66
-
 2-oxoglutarate pH 7.0, 38°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.33
-
 2-Oxohexanoate pH 7.0, 38°C Mus musculus
1.33
-
 L-phenylalanine pH 7.0, 38°C Mus musculus
2
-
 L-glutamate pH 7.0, 38°C Mus musculus
3
-
 phenylpyruvate pH 7.0, 38°C Mus musculus
10.66
-
 L-tyrosine pH 7.0, 38°C Mus musculus
14.16
-
 2-oxoglutarate pH 7.0, 38°C Mus musculus
22.66
-
 4-hydroxyphenylpyruvate pH 7.0, 38°C Mus musculus