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Literature summary for 2.6.1.5 extracted from

  • De-Eknamkul, W.; Ellis, B.E.
    Purification and characterization of tyrosine aminotransferase activities from Anchusa officinalis cell cultures (1987), Arch. Biochem. Biophys., 257, 430-438.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
3,4-Dihydroxyphenyllactate strong inhibition of all the three TAT isoenzymes Anchusa officinalis
rosmarinic acid isoenzymes TAT-2 and TAT-3 Anchusa officinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
L-tyrosine pH 9.2, 40°C, isoenzyme TAT-3 Anchusa officinalis
5
-
L-tyrosine pH 9.2, 40°C, isoenzyme TAT-2 Anchusa officinalis
20
-
L-tyrosine pH 9.2, 40°C, isoenzyme TAT-1 Anchusa officinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
4 * 43000, isoenzyme TAT-1, SDS-PAGE Anchusa officinalis
56000
-
4 * 56000, isoenzyme TAT-2, SDS-PAGE Anchusa officinalis
180000
-
isoenzyme TAT-1, gel filtration Anchusa officinalis
220000
-
isoenzymes TAT-2 and TAT-3, gel filtration Anchusa officinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine + 2-oxoglutarate Anchusa officinalis first step in tyrosine pathway leading to the formation of rosmarinic acid (alpha-O-caffeoyl-3,4-dihydroxyphenyllactic acid) 4-hydroxyphenylpyruvate + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Anchusa officinalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
three isoenzymes TAT-1, TAT-2 and TAT-3, isolated using anion-exchange chromatography and electrofocusing Anchusa officinalis

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Anchusa officinalis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activities for different isoenzymes Anchusa officinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate isoenzymes TAT-2 and TAT-3, best substrate for isoenzyme TAT-3 Anchusa officinalis oxaloacetate + L-glutamate
-
?
L-tyrosine + 2-oxoglutarate isoenzymes TAT-1, TAT-2 and TAT-3 show a pronounced preference for L-tyrosine over other aromatic amino acids Anchusa officinalis 4-hydroxyphenylpyruvate + L-glutamate
-
?
L-tyrosine + 2-oxoglutarate isoenzyme TAT-1: oxaloacetate and 2-oxoglutarate utilized equally well as amino acceptor, isoenzyme TAT-2: oxaloacetate is most effective, isoenzyme TAT-3: 2-oxoglutarate is most effective Anchusa officinalis 4-hydroxyphenylpyruvate + L-glutamate
-
?
L-tyrosine + 2-oxoglutarate first step in tyrosine pathway leading to the formation of rosmarinic acid (alpha-O-caffeoyl-3,4-dihydroxyphenyllactic acid) Anchusa officinalis 4-hydroxyphenylpyruvate + L-glutamate
-
?
L-tyrosine + oxaloacetate
-
Anchusa officinalis 4-hydroxyphenylpyruvate + L-aspartate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 43000, isoenzyme TAT-1, SDS-PAGE Anchusa officinalis
tetramer 4 * 56000, isoenzyme TAT-2, SDS-PAGE Anchusa officinalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
assay at Anchusa officinalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
15 min, 50% loss of activity, isoenzyme TAT-3 Anchusa officinalis
55
-
15 min, 50% loss of activity, isoenzyme TAT-2 Anchusa officinalis
65
-
15 min, 50% loss of activity, isoenzyme TAT-1 Anchusa officinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
optimum pH for the isoenzyme TAT-2 is above pH 9.6 Anchusa officinalis
9
-
L-tyrosine as substrate, isoenzyme TAT-1 Anchusa officinalis

pH Range

pH Minimum pH Maximum Comment Organism
8.8 9.4 L-tyrosine as substrate, isoenzyme TAT-3 Anchusa officinalis