Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-Dihydroxyphenyllactate | strong inhibition of all the three TAT isoenzymes | Anchusa officinalis | |
rosmarinic acid | isoenzymes TAT-2 and TAT-3 | Anchusa officinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
L-tyrosine | pH 9.2, 40°C, isoenzyme TAT-3 | Anchusa officinalis | |
5 | - |
L-tyrosine | pH 9.2, 40°C, isoenzyme TAT-2 | Anchusa officinalis | |
20 | - |
L-tyrosine | pH 9.2, 40°C, isoenzyme TAT-1 | Anchusa officinalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
4 * 43000, isoenzyme TAT-1, SDS-PAGE | Anchusa officinalis |
56000 | - |
4 * 56000, isoenzyme TAT-2, SDS-PAGE | Anchusa officinalis |
180000 | - |
isoenzyme TAT-1, gel filtration | Anchusa officinalis |
220000 | - |
isoenzymes TAT-2 and TAT-3, gel filtration | Anchusa officinalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + 2-oxoglutarate | Anchusa officinalis | first step in tyrosine pathway leading to the formation of rosmarinic acid (alpha-O-caffeoyl-3,4-dihydroxyphenyllactic acid) | 4-hydroxyphenylpyruvate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anchusa officinalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
three isoenzymes TAT-1, TAT-2 and TAT-3, isolated using anion-exchange chromatography and electrofocusing | Anchusa officinalis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Anchusa officinalis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activities for different isoenzymes | Anchusa officinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | isoenzymes TAT-2 and TAT-3, best substrate for isoenzyme TAT-3 | Anchusa officinalis | oxaloacetate + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | isoenzymes TAT-1, TAT-2 and TAT-3 show a pronounced preference for L-tyrosine over other aromatic amino acids | Anchusa officinalis | 4-hydroxyphenylpyruvate + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | isoenzyme TAT-1: oxaloacetate and 2-oxoglutarate utilized equally well as amino acceptor, isoenzyme TAT-2: oxaloacetate is most effective, isoenzyme TAT-3: 2-oxoglutarate is most effective | Anchusa officinalis | 4-hydroxyphenylpyruvate + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | first step in tyrosine pathway leading to the formation of rosmarinic acid (alpha-O-caffeoyl-3,4-dihydroxyphenyllactic acid) | Anchusa officinalis | 4-hydroxyphenylpyruvate + L-glutamate | - |
? | |
L-tyrosine + oxaloacetate | - |
Anchusa officinalis | 4-hydroxyphenylpyruvate + L-aspartate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 43000, isoenzyme TAT-1, SDS-PAGE | Anchusa officinalis |
tetramer | 4 * 56000, isoenzyme TAT-2, SDS-PAGE | Anchusa officinalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Anchusa officinalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
15 min, 50% loss of activity, isoenzyme TAT-3 | Anchusa officinalis |
55 | - |
15 min, 50% loss of activity, isoenzyme TAT-2 | Anchusa officinalis |
65 | - |
15 min, 50% loss of activity, isoenzyme TAT-1 | Anchusa officinalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
optimum pH for the isoenzyme TAT-2 is above pH 9.6 | Anchusa officinalis |
9 | - |
L-tyrosine as substrate, isoenzyme TAT-1 | Anchusa officinalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.8 | 9.4 | L-tyrosine as substrate, isoenzyme TAT-3 | Anchusa officinalis |