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Literature summary for 2.6.1.49 extracted from
- Purification and properties of dihydroxyphenylalanine transaminase from guinea pig brain (1965), J. Neurochem., 12, 589-598.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal phosphate | a pyridoxal phosphate protein, Km: 0.016 mM | Cavia porcellus |  |
General Stability
| General Stability | Organism |
|---|---|
| pyridoxal phosphate, Ca2+, Mg2+ or Mn2+ protects against heat inactivation | Cavia porcellus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetic acid | 1 mM, 75% inhibition | Cavia porcellus | |
| KCN | 1 mM, 93% inhibition, pyridoxal 5'-phosphate protects | Cavia porcellus | |
| p-chloromercuriphenyl sulfonic acid | 0.02 mM, 92% inhibition, addition of 1 mM glutathione protects to 100% | Cavia porcellus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.48 | - |
2-oxoglutarate | pH 8.2, 37°C | Cavia porcellus | |
| 41 | - |
L-Dopa | pH 8.2, 37°C | Cavia porcellus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, 52°C, calcium phosphate gel, alumina C, Sephadex G-200 | Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0366 | - |
- |
Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-(3,4-dihydroxyphenyl)-L-alanine + 2-oxoglutarate | i.e. L-dopa | Cavia porcellus | 3-(3,4-dihydroxyphenyl)pyruvate + L-glutamate | i.e. DOPP | ? | |
| 3-(3,4-dihydroxyphenyl)-L-alanine + 2-oxoglutarate | most effective amino acceptor | Cavia porcellus | 3-(3,4-dihydroxyphenyl)pyruvate + L-glutamate | i.e. DOPP | ? | |
| 3-(3,4-dihydroxyphenyl)-L-alanine + oxaloacetic acid | i.e. L-Dopa, 10% of activity with 2-oxoglutarate | Cavia porcellus | 3-(3,4-dihydroxyphenyl)pyruvate + L-aspartate | - |
? | |
| L-tyrosine + 2-oxoglutarate | 8% of activity with L-Dopa | Cavia porcellus | 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Cavia porcellus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
pyridoxal phosphate, Ca2+, Mg2+ or Mn2+ protects against heat inactivation | Cavia porcellus |
| 55 | - |
6 min, pH 7.0, stable | Cavia porcellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Cavia porcellus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | approx. 35% of maximal activity at pH 7.0, approx. 60% of activity at pH 9.0 | Cavia porcellus |
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