BRENDA - Enzyme Database show
show all sequences of 2.6.1.46

Characterization of biosynthetic enzymes for ectoine as a compatible solute in a moderately halophilic eubacterium, Halomonas elongata

Ono, H.; Sawada, K.; Khunajakr, N.; Tao, T.; Yamamoto, M.; Hiramoto, M.; Shinmyo, A.; Takano, M.; Murooka, Y.; J. Bacteriol. 181, 91-99 (1999)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.5
-
L-2-amino-4-oxobutanoate
pH 8.5, 25ºC
Halomonas elongata
9.1
-
L-glutamate
pH 8.5, 25ºC
Halomonas elongata
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
required for activity and stability
Halomonas elongata
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
gel filtration
Halomonas elongata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-2-amino-4-oxobutanoate + L-glutamate
Halomonas elongata
-
L-2,4-diaminobutanoate + 2-oxoglutarate
-
Halomonas elongata
r
L-2-amino-4-oxobutanoate + L-glutamate
Halomonas elongata OUT300318
-
L-2,4-diaminobutanoate + 2-oxoglutarate
-
Halomonas elongata OUT300318
r
L-2-amino-4-oxobutanoate + L-glutamate
Halomonas elongata OUT30018
-
L-2,4-diaminobutanoate + 2-oxoglutarate
-
Halomonas elongata OUT30018
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Halomonas elongata
-
-
-
Halomonas elongata OUT300318
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, Sepharose CL-6B
Halomonas elongata
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Halomonas elongata
Storage Stability
Storage Stability
Organism
0ºC, 100 mM 2,4-diaminobutyric acid
Halomonas elongata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-2-amino-4-oxobutanoate + L-glutamate
-
640091
Halomonas elongata
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640091
Halomonas elongata
r
L-2-amino-4-oxobutanoate + L-glutamate
-
640091
Halomonas elongata OUT300318
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640091
Halomonas elongata OUT300318
r
L-2-amino-4-oxobutanoate + L-glutamate
-
640091
Halomonas elongata OUT30018
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640091
Halomonas elongata OUT30018
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
in presence of 0.4 M NaCl
Halomonas elongata
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
8.7
-
Halomonas elongata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.5
-
L-2-amino-4-oxobutanoate
pH 8.5, 25ºC
Halomonas elongata
9.1
-
L-glutamate
pH 8.5, 25ºC
Halomonas elongata
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
required for activity and stability
Halomonas elongata
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
gel filtration
Halomonas elongata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-2-amino-4-oxobutanoate + L-glutamate
Halomonas elongata
-
L-2,4-diaminobutanoate + 2-oxoglutarate
-
Halomonas elongata
r
L-2-amino-4-oxobutanoate + L-glutamate
Halomonas elongata OUT300318
-
L-2,4-diaminobutanoate + 2-oxoglutarate
-
Halomonas elongata OUT300318
r
L-2-amino-4-oxobutanoate + L-glutamate
Halomonas elongata OUT30018
-
L-2,4-diaminobutanoate + 2-oxoglutarate
-
Halomonas elongata OUT30018
r
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, Sepharose CL-6B
Halomonas elongata
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Halomonas elongata
Storage Stability (protein specific)
Storage Stability
Organism
0ºC, 100 mM 2,4-diaminobutyric acid
Halomonas elongata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-2-amino-4-oxobutanoate + L-glutamate
-
640091
Halomonas elongata
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640091
Halomonas elongata
r
L-2-amino-4-oxobutanoate + L-glutamate
-
640091
Halomonas elongata OUT300318
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640091
Halomonas elongata OUT300318
r
L-2-amino-4-oxobutanoate + L-glutamate
-
640091
Halomonas elongata OUT30018
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640091
Halomonas elongata OUT30018
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
in presence of 0.4 M NaCl
Halomonas elongata
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
8.7
-
Halomonas elongata
Other publictions for EC 2.6.1.46
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
660836
Reshetnikov
Characterization of the ectoin ...
Methylomicrobium alcaliphilum, Methylomicrobium alcaliphilum 20Z
Arch. Microbiol.
184
286-297
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659047
Vandenende
Functional characterization of ...
Pseudomonas aeruginosa
J. Bacteriol.
186
5596-5602
2004
-
-
1
-
-
-
-
5
-
1
2
1
-
1
-
-
1
1
-
-
1
-
4
1
1
-
-
2
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
5
-
1
2
1
-
-
-
1
-
-
1
-
4
1
1
-
-
2
1
-
-
-
-
-
-
-
-
-
640091
Ono
Characterization of biosynthet ...
Halomonas elongata, Halomonas elongata OUT300318
J. Bacteriol.
181
91-99
1999
-
-
-
-
-
-
-
2
-
1
1
3
-
2
-
-
1
-
-
-
1
1
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
1
3
-
-
-
1
-
-
1
1
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
640090
Peters
-
The biosynthesis of ectoine ...
Halomonas elongata, Halorhodospira halochloris
FEMS Microbiol. Lett.
71
157-162
1990
-
-
-
-
-
-
-
3
-
-
-
2
-
2
-
-
-
-
-
-
2
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
2
-
-
-
-
-
-
2
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
640089
Rajagopal Rao
A study of the metabolism of L ...
Xanthomonas sp.
Biochem. J.
114
107-115
1969
-
-
-
-
-
-
4
-
-
1
-
1
-
1
-
-
1
-
-
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
1
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-