BRENDA - Enzyme Database show
show all sequences of 2.6.1.46

The biosynthesis of ectoine

Peters, P.; Galinski, E.A.; Trüper, H.G.; FEMS Microbiol. Lett. 71, 157-162 (1990)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2
-
L-glutamate
pH 9.1, 37ºC
Halorhodospira halochloris
1.85
-
L-glutamate
-
Halomonas elongata
1.85
-
L-glutamate
pH 9.1, 37ºC
Halorhodospira halochloris
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-2-amino-4-oxobutanoate + L-glutamate
Halorhodospira halochloris
involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria
L-2,4-diaminobutanoate + 2-oxoglutarate
-
Halorhodospira halochloris
?
L-2-amino-4-oxobutanoate + L-glutamate
Halomonas elongata
involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria
L-2,4-diaminobutanoate + 2-oxoglutarate
-
Halomonas elongata
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Halomonas elongata
-
strain DSM 2581
-
Halorhodospira halochloris
-
strain DSM 1059
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.054
-
-
Halorhodospira halochloris
0.171
-
-
Halomonas elongata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-2-amino-4-oxobutanoate + L-glutamate
-
640090
Halorhodospira halochloris
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640090
Halorhodospira halochloris
?
L-2-amino-4-oxobutanoate + L-glutamate
-
640090
Halomonas elongata
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640090
Halomonas elongata
?
L-2-amino-4-oxobutanoate + L-glutamate
involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria
640090
Halorhodospira halochloris
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640090
Halorhodospira halochloris
?
L-2-amino-4-oxobutanoate + L-glutamate
involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria
640090
Halomonas elongata
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640090
Halomonas elongata
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Halomonas elongata
37
-
assay at
Halorhodospira halochloris
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.9
-
-
Halomonas elongata
8.2
-
-
Halorhodospira halochloris
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2
-
L-glutamate
pH 9.1, 37ºC
Halorhodospira halochloris
1.85
-
L-glutamate
-
Halomonas elongata
1.85
-
L-glutamate
pH 9.1, 37ºC
Halorhodospira halochloris
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-2-amino-4-oxobutanoate + L-glutamate
Halorhodospira halochloris
involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria
L-2,4-diaminobutanoate + 2-oxoglutarate
-
Halorhodospira halochloris
?
L-2-amino-4-oxobutanoate + L-glutamate
Halomonas elongata
involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria
L-2,4-diaminobutanoate + 2-oxoglutarate
-
Halomonas elongata
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.054
-
-
Halorhodospira halochloris
0.171
-
-
Halomonas elongata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-2-amino-4-oxobutanoate + L-glutamate
-
640090
Halorhodospira halochloris
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640090
Halorhodospira halochloris
?
L-2-amino-4-oxobutanoate + L-glutamate
-
640090
Halomonas elongata
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640090
Halomonas elongata
?
L-2-amino-4-oxobutanoate + L-glutamate
involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria
640090
Halorhodospira halochloris
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640090
Halorhodospira halochloris
?
L-2-amino-4-oxobutanoate + L-glutamate
involved in the biosynthesis of the osmolyte ectoine in halophilic eubacteria
640090
Halomonas elongata
L-2,4-diaminobutanoate + 2-oxoglutarate
-
640090
Halomonas elongata
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Halomonas elongata
37
-
assay at
Halorhodospira halochloris
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.9
-
-
Halomonas elongata
8.2
-
-
Halorhodospira halochloris
Other publictions for EC 2.6.1.46
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
660836
Reshetnikov
Characterization of the ectoin ...
Methylomicrobium alcaliphilum, Methylomicrobium alcaliphilum 20Z
Arch. Microbiol.
184
286-297
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659047
Vandenende
Functional characterization of ...
Pseudomonas aeruginosa
J. Bacteriol.
186
5596-5602
2004
-
-
1
-
-
-
-
5
-
1
2
1
-
1
-
-
1
1
-
-
1
-
4
1
1
-
-
2
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
5
-
1
2
1
-
-
-
1
-
-
1
-
4
1
1
-
-
2
1
-
-
-
-
-
-
-
-
-
640091
Ono
Characterization of biosynthet ...
Halomonas elongata, Halomonas elongata OUT300318
J. Bacteriol.
181
91-99
1999
-
-
-
-
-
-
-
2
-
1
1
3
-
2
-
-
1
-
-
-
1
1
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
1
3
-
-
-
1
-
-
1
1
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
640090
Peters
-
The biosynthesis of ectoine ...
Halomonas elongata, Halorhodospira halochloris
FEMS Microbiol. Lett.
71
157-162
1990
-
-
-
-
-
-
-
3
-
-
-
2
-
2
-
-
-
-
-
-
2
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
2
-
-
-
-
-
-
2
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
640089
Rajagopal Rao
A study of the metabolism of L ...
Xanthomonas sp.
Biochem. J.
114
107-115
1969
-
-
-
-
-
-
4
-
-
1
-
1
-
1
-
-
1
-
-
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
1
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-