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Literature summary for 2.6.1.45 extracted from
- Purification and properties of an asparagine aminotransferase from Pisum sativum leaves (1983), Arch. Biochem. Biophys., 223, 291-296.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Aminooxyacetate | 1 mM, complete inhibition | Pisum sativum |  |
| glyoxylate | competitive vs. pyruvate | Pisum sativum | |
| hydroxylamine | 0.1 mM, 97% inhibition, 85% inhibition in the presence of 0.1 mM pyridoxal phosphate | Pisum sativum | |
| L-asparagine | competitive vs. serine | Pisum sativum | |
| L-serine | competitive vs. asparagine | Pisum sativum | |
| pyruvate | competitive vs. glyoxylate | Pisum sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
L-serine | pH 8.1, 30°C, cosubstrate pyruvate | Pisum sativum | |
| 0.6 | - |
L-serine | pH 8.1, 30°C, cosubstrate glyoxylate | Pisum sativum | |
| 2 | - |
pyruvate | pH 8.1, 30°C, cosubstrate L-serine | Pisum sativum | |
| 2.5 | - |
pyruvate | pH 8.1, 30°C, cosubstrates: L-asparagine, glyoxylate, L-asparagine | Pisum sativum | |
| 3.9 | - |
L-asparagine | pH 8.1, 30°C, cosubstrate glyoxylate | Pisum sativum | |
| 4.5 | - |
L-asparagine | pH 8.1, 30°C, cosubstrate pyruvate | Pisum sativum | |
| 4.6 | - |
glyoxylate | pH 8.1, 30°C, cosubstrate L-serine | Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peroxisome | - |
Pisum sativum | 5777 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 105000 | - |
gel filtration | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + glyoxylate | Pisum sativum | - |
3-hydroxypyruvate + glycine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
pea, cv. Little Marvel | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| pH 5.4, polyethylene glycol 6000, DEAE-Sephadex A25, Sephacryl S-300, partial purification | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 39.3 | - |
- |
Pisum sativum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, stable overnight, 20% loss of activity per day on further storage, in 20% ethylene glycol or 15% glycerol, 20% loss of activity within 5 days | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + glyoxylate | transamination at 38% the rate of L-serine | Pisum sativum | 2-oxosuccinamate + glycine | - |
r | |
| L-serine + 2-oxosuccinamate | poor substrate | Pisum sativum | 3-hydroxypyruvate + L-asparagine | - |
? | |
| L-serine + glyoxylate | - |
Pisum sativum | 3-hydroxypyruvate + glycine | - |
? | |
| L-serine + glyoxylate | no activity with Phe, Arg, Val, Trp, Thr, Met | Pisum sativum | 3-hydroxypyruvate + glycine | - |
r | |
| L-serine + glyoxylate | reverse reaction at 4-11% the rate of forward reaction | Pisum sativum | 3-hydroxypyruvate + glycine | - |
r | |
| L-serine + glyoxylate | preferred substrates | Pisum sativum | 3-hydroxypyruvate + glycine | - |
r | |
| L-serine + glyoxylate | no or trace activity with oxaloacetate, 2-oxoglutarate | Pisum sativum | 3-hydroxypyruvate + glycine | - |
r | |
| L-serine + glyoxylate | no activity with Tyr, Ile, Pro, Cys, Leu, Asp | Pisum sativum | 3-hydroxypyruvate + glycine | - |
r | |
| L-serine + glyoxylate | equilibrium towards glycine production | Pisum sativum | 3-hydroxypyruvate + glycine | - |
r | |
| L-serine + hydroxypyruvate | poor substrate | Pisum sativum | 3-hydroxypyruvate + L-serine | - |
? | |
| L-serine + pyruvate | 8% of glyoxylate transamination activity | Pisum sativum | 3-hydroxypyruvate + L-alanine | - |
r | |
| L-serine + pyruvate | equilibrium towards alanine production | Pisum sativum | 3-hydroxypyruvate + L-alanine | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
- |
Pisum sativum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.2 | approx. 50% activity at pH 7.5 and 9.2, no activity below pH 6.7 and above pH 9.9 | Pisum sativum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | required for activity | Pisum sativum | |
| pyridoxal 5'-phosphate | Km-value: 0.0025 mM | Pisum sativum | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
L-serine | pH 8.1, 30°C | Pisum sativum | |
| 0.4 | - |
glyoxylate | pH 8.1, 30°C | Pisum sativum | |
| 3 | - |
L-asparagine | pH 8.1, 30°C | Pisum sativum | |
| 25 | - |
pyruvate | pH 8.1, 30°C | Pisum sativum | |
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