General Stability | Organism |
---|---|
stability is improved by 10-15% glycerol | Chlorella regularis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Chlorella regularis | |
4,5-Dioxopentanoate | substrate inhibition | Chlorella regularis | |
amino-oxyacetic acid | - |
Chlorella regularis | |
glyoxylic acid | - |
Chlorella regularis | |
imidazole | - |
Chlorella regularis | |
pyruvate | - |
Chlorella regularis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
4,5-Dioxopentanoate | pH 8.0, 37°C | Chlorella regularis | |
1.3 | - |
glyoxylate | pH 8.0, 37°C | Chlorella regularis | |
3 | - |
L-alanine | pH 8.0, 37°C, glyoxylate as amino acceptor | Chlorella regularis | |
3.5 | - |
L-alanine | pH 8.0, 37°C | Chlorella regularis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
2 * 68000, SDS-PAGE | Chlorella regularis |
126000 | - |
gel filtration | Chlorella regularis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + 4,5-dioxopentanoate | Chlorella regularis | - |
5-aminolevulinate + pyruvate | - |
ir | |
L-alanine + 4,5-dioxopentanoate | Chlorella regularis S-50 | - |
5-aminolevulinate + pyruvate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorella regularis | - |
S-50 | - |
Chlorella regularis S-50 | - |
S-50 | - |
Purification (Comment) | Organism |
---|---|
- |
Chlorella regularis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.68 | - |
- |
Chlorella regularis |
Storage Stability | Organism |
---|---|
4°C, 10-15% glycerol, 20% loss of activity after 5 days | Chlorella regularis |
4°C, unstable | Chlorella regularis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + 4,5-dioxopentanoate | 21% of the activity with L-alanine | Chlorella regularis | 5-aminolevulinate + glyoxylate | - |
ir | |
glycine + 4,5-dioxopentanoate | 21% of the activity with L-alanine | Chlorella regularis S-50 | 5-aminolevulinate + glyoxylate | - |
ir | |
L-alanine + 4,5-dioxopentanoate | - |
Chlorella regularis | 5-aminolevulinate + pyruvate | - |
ir | |
L-alanine + 4,5-dioxopentanoate | - |
Chlorella regularis S-50 | 5-aminolevulinate + pyruvate | - |
ir | |
L-glutamate + 4,5-dioxopentanoate | 37% of the activity with L-alanine | Chlorella regularis | 5-aminolevulinate + 2-oxoglutarate | - |
ir | |
L-glutamate + 4,5-dioxopentanoate | 37% of the activity with L-alanine | Chlorella regularis S-50 | 5-aminolevulinate + 2-oxoglutarate | - |
ir | |
L-glutamine + 4,5-dioxopentanoate | 16% of the activity with L-alanine | Chlorella regularis | 5-aminolevulinate + 2-oxoglutaramate | - |
? | |
L-lysine + 4,5-dioxopentanoate | 15% of the activity with L-alanine | Chlorella regularis | 5-aminolevulinate + 6-amino-2-oxohexanoate | - |
ir | |
L-serine + 4,5-dioxopentanoate | 33% of the activity with L-alanine | Chlorella regularis | 5-aminolevulinate + 3-hydroxy-2-oxopropanoate | - |
ir | |
L-serine + 4,5-dioxopentanoate | 33% of the activity with L-alanine | Chlorella regularis S-50 | 5-aminolevulinate + 3-hydroxy-2-oxopropanoate | - |
ir | |
additional information | does not use 2-oxoglutarate as amino acceptor | Chlorella regularis | ? | - |
? | |
additional information | does not use 2-oxoglutarate as amino acceptor | Chlorella regularis S-50 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 68000, SDS-PAGE | Chlorella regularis |
Synonyms | Comment | Organism |
---|---|---|
EC 2.6.1.44 | identical with alanine-glyoxylate aminotransferase | Chlorella regularis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
retains 75% activity after 1 h incubation | Chlorella regularis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Chlorella regularis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
glyoxylate | pH 8.0, 37°C | Chlorella regularis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Chlorella regularis | isoelectrofocusing column 110 ml and Pharmalyte gradient pH 3-10 at 4°C for 45 h | - |
4.8 |