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Literature summary for 2.6.1.43 extracted from

  • Shioi, Y.; Nagamine, M.; Sasa, T.
    Purification and properties of L-alanine:4,5-dioxovalerate aminotransferase from Chlorella regularis (1984), Arch. Biochem. Biophys., 234, 117-124.
    View publication on PubMed

General Stability

General Stability Organism
stability is improved by 10-15% glycerol Chlorella regularis

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate
-
Chlorella regularis
4,5-Dioxopentanoate substrate inhibition Chlorella regularis
amino-oxyacetic acid
-
Chlorella regularis
glyoxylic acid
-
Chlorella regularis
imidazole
-
Chlorella regularis
pyruvate
-
Chlorella regularis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
4,5-Dioxopentanoate pH 8.0, 37°C Chlorella regularis
1.3
-
glyoxylate pH 8.0, 37°C Chlorella regularis
3
-
L-alanine pH 8.0, 37°C, glyoxylate as amino acceptor Chlorella regularis
3.5
-
L-alanine pH 8.0, 37°C Chlorella regularis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
2 * 68000, SDS-PAGE Chlorella regularis
126000
-
gel filtration Chlorella regularis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + 4,5-dioxopentanoate Chlorella regularis
-
5-aminolevulinate + pyruvate
-
ir
L-alanine + 4,5-dioxopentanoate Chlorella regularis S-50
-
5-aminolevulinate + pyruvate
-
ir

Organism

Organism UniProt Comment Textmining
Chlorella regularis
-
S-50
-
Chlorella regularis S-50
-
S-50
-

Purification (Commentary)

Purification (Comment) Organism
-
Chlorella regularis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.68
-
-
Chlorella regularis

Storage Stability

Storage Stability Organism
4°C, 10-15% glycerol, 20% loss of activity after 5 days Chlorella regularis
4°C, unstable Chlorella regularis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycine + 4,5-dioxopentanoate 21% of the activity with L-alanine Chlorella regularis 5-aminolevulinate + glyoxylate
-
ir
glycine + 4,5-dioxopentanoate 21% of the activity with L-alanine Chlorella regularis S-50 5-aminolevulinate + glyoxylate
-
ir
L-alanine + 4,5-dioxopentanoate
-
Chlorella regularis 5-aminolevulinate + pyruvate
-
ir
L-alanine + 4,5-dioxopentanoate
-
Chlorella regularis S-50 5-aminolevulinate + pyruvate
-
ir
L-glutamate + 4,5-dioxopentanoate 37% of the activity with L-alanine Chlorella regularis 5-aminolevulinate + 2-oxoglutarate
-
ir
L-glutamate + 4,5-dioxopentanoate 37% of the activity with L-alanine Chlorella regularis S-50 5-aminolevulinate + 2-oxoglutarate
-
ir
L-glutamine + 4,5-dioxopentanoate 16% of the activity with L-alanine Chlorella regularis 5-aminolevulinate + 2-oxoglutaramate
-
?
L-lysine + 4,5-dioxopentanoate 15% of the activity with L-alanine Chlorella regularis 5-aminolevulinate + 6-amino-2-oxohexanoate
-
ir
L-serine + 4,5-dioxopentanoate 33% of the activity with L-alanine Chlorella regularis 5-aminolevulinate + 3-hydroxy-2-oxopropanoate
-
ir
L-serine + 4,5-dioxopentanoate 33% of the activity with L-alanine Chlorella regularis S-50 5-aminolevulinate + 3-hydroxy-2-oxopropanoate
-
ir
additional information does not use 2-oxoglutarate as amino acceptor Chlorella regularis ?
-
?
additional information does not use 2-oxoglutarate as amino acceptor Chlorella regularis S-50 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 68000, SDS-PAGE Chlorella regularis

Synonyms

Synonyms Comment Organism
EC 2.6.1.44 identical with alanine-glyoxylate aminotransferase Chlorella regularis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
retains 75% activity after 1 h incubation Chlorella regularis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Chlorella regularis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.5
-
glyoxylate pH 8.0, 37°C Chlorella regularis

pI Value

Organism Comment pI Value Maximum pI Value
Chlorella regularis isoelectrofocusing column 110 ml and Pharmalyte gradient pH 3-10 at 4°C for 45 h
-
4.8