Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glyoxylic acid | competitive inhibition | Bos taurus | |
hydroxylamine | - |
Bos taurus | |
Isonicotinic acid hydrazide | - |
Bos taurus | |
potassium cyanide | - |
Bos taurus | |
Semicarbazide | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
4,5-Dioxopentanoate | pH 7.0, 37°C | Bos taurus | |
0.67 | - |
glyoxylate | pH 7.0, 37°C | Bos taurus | |
2.5 | - |
L-alanine | pH 7.0, 37°C, 4,5-dioxopentanoate as amino acceptor | Bos taurus | |
4 | - |
L-alanine | pH 7.0, 37°C, glyoxylate as amino acceptor | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
4 * 58000, SDS-PAGE | Bos taurus |
238000 | - |
gel filtration | Bos taurus |
240000 | - |
- |
Bos taurus |
245000 | - |
sucrose density gradient centrifugation | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + 4,5-dioxopentanoate | Bos taurus | biosynthesis of porphyrin precursors | 5-aminolevulinate + pyruvate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
bovine | - |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.753 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme in 50 mM potassium phosphate buffer, pH 7.5, 10% glycerol, may be stored for at least 8 weeks without loss of either activity | Bos taurus |
0-5°C, 50 mM potassium phosphate buffer, pH 7.5, 10% glycerol, stored for 2 weeks, little or none of each activity is lost | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + 4,5-dioxopentanoate | - |
Bos taurus | 5-aminolevulinate + glyoxylate | - |
? | |
L-alanine + 4,5-dioxopentanoate | - |
Bos taurus | 5-aminolevulinate + pyruvate | - |
ir | |
L-alanine + 4,5-dioxopentanoate | biosynthesis of porphyrin precursors | Bos taurus | 5-aminolevulinate + pyruvate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 58000, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
EC 2.6.1.44 | alanine-glyoxylate transaminase and aminolevulinate transaminase activities reside in the same protein | Bos taurus |
EC 2.6.1.44 | identical with alanine-glyoxylate aminotransferase | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, about 65% loss of activity | Bos taurus |