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Literature summary for 2.6.1.43 extracted from

  • Varticovski, L.; Kushner, J.P.; Burnham, B.F.
    Biosynthesis of porphyrin precursors. Purification and characterization of mammalian L-alanine:gamma,delta-dioxovaleric acid aminotransferase (1980), J. Biol. Chem., 255, 3742-3747.
    View publication on PubMed
Search for more literature for 2.6.1.43

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Bos taurus

General Stability

General Stability Organism
freezing and thawing does not affect activity Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate
-
 Bos taurus
5-aminolevulinate
-
 Bos taurus
methylglyoxal
-
 Bos taurus
pyruvate
-
 Bos taurus
succinate semialdehyde
-
 Bos taurus
Tris-HCl
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 4,5-Dioxopentanoate pH 6.9, 37°C Bos taurus
3.7
-
 L-alanine pH 6.9, 37°C Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosol contains minimal enzymic activity Bos taurus 5829
-
mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
240000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + 4,5-dioxopentanoate Bos taurus
-
 5-aminolevulinate + pyruvate
-
 ir

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 bovine
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.16
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-alanine + 4,5-dioxopentanoate 23% of the activity with L-alanine Bos taurus 5-aminolevulinate + malonic semialdehyde
-
 ir
D-alanine + 4,5-dioxopentanoate 3% of the activity with L-alanine Bos taurus 5-aminolevulinate + pyruvate
-
 ir
delta-amino-n-valerate + 4,5-dioxopentanoate 10% of the activity with L-alanine Bos taurus 5-aminolevulinate + 5-oxo-n-pentanoate
-
 ir
DL-alanine + 4,5-dioxopentanoate 89% of the activity with L-alanine Bos taurus 5-aminolevulinate + pyruvate
-
 ir
gamma-amino-n-butyrate + 4,5-dioxopentanoate 10% of the activity with L-alanine Bos taurus 5-aminolevulinate + succinic semialdehyde
-
 ir
glycine + 4,5-dioxopentanoate 9% of the activity with L-alanine Bos taurus 5-aminolevulinate + glyoxylate
-
 ir
L-alanine + 4,5-dioxopentanoate
-
 Bos taurus 5-aminolevulinate + pyruvate
-
 ir
L-lysine + 4,5-dioxopentanoate 9% of the activity with L-alanine Bos taurus 5-aminolevulinate + 6-amino-2-oxohexanoate
-
 ir
L-ornithine + 4,5-dioxopentanoate 6% of the activity with L-alanine Bos taurus 5-aminolevulinate + 5-amino-2-oxopentanoate
-
 ir
additional information glutamate is no substrate, transamination of methylglyoxal yields aminoacetone, less than 1% efficient as amino acceptor as 4,5-dioxopentanoate Bos taurus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
-
 Bos taurus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Bos taurus