Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.42 extracted from

  • Roncador, A.; Oppici, E.; Montioli, R.; Maset, F.; Cellini, B.
    TAT-Mediated delivery of human alanine:glyoxylate aminotransferase in a cellular model of primary hyperoxaluria type I (2013), Int. J. Pept. Res. Ther., 19, 175-184.
No PubMed abstract available
Search for more literature for 2.6.1.42

Application

Application Comment Organism
medicine a fusion protein of AGT with the membrane-penetrating Tat peptide shows subtle active site conformational changes as compared with untagged AGT, but retains a significant transaminase activity. Active Tat-AGT can be successfully delivered to a mammalian cellular model of primary hyperoxaluria type I consisting of chinese hamster ovary cells expressing glycolate oxidase. The intracellular transduced Tat-AGT makes the cells able to detoxify endogenously produced glyoxylate to an extent similar to that of cells stably expressing AGT Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 glyoxylate wild-type, pH 7.4, 25°C Homo sapiens
0.26
-
 glyoxylate TAT-peptide fusion protein, pH 7.4, 25°C Homo sapiens
35
-
 L-alanine wild-type, pH 7.4, 25°C Homo sapiens
74
-
 L-alanine TAT-peptide fusion protein, pH 7.4, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + glyoxylate
-
 Homo sapiens pyruvate + glycine
-
 ?

Synonyms

Synonyms Comment Organism
AGT
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16
-
 glyoxylate TAT-peptide fusion protein, pH 7.4, 25°C Homo sapiens
17
-
 L-alanine TAT-peptide fusion protein, pH 7.4, 25°C Homo sapiens
42
-
 glyoxylate wild-type, pH 7.4, 25°C Homo sapiens
50
-
 L-alanine wild-type, pH 7.4, 25°C Homo sapiens