Any feedback?
Literature summary for 2.6.1.42 extracted from
- Regulatory control of human cytosolic branched-chain aminotransferase by oxidation and S-glutathionylation and its interactions with redox sensitive neuronal proteins (2008), Biochemistry, 47, 5465-5479.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C221S | the mutant shows strongly decreased kcat values compared to the wild type enzyme | Homo sapiens |
| C235S | the mutant enzyme exhibits kcat values that are within 20-30% of wild type BCATc values, but also have lower Km values, hence higher calculated kcat/Km values than those of the wild type enzyme | Homo sapiens |
| C242S | the mutant shows strongly decreased kcat values compared to the wild type enzyme | Homo sapiens |
| C293S | the C293S mutant and wild type BCATc have similar kinetic constants with L-valine, L-leucine, and L-glutamate | Homo sapiens |
| C335S | for all amino acid substrates, there is a significant decrease (70% to 80%) in kcat with the C335S mutant, the Km value for glutamate with the C335S mutant enzyme is approximately 3fold lower than that observed with wild type enzyme | Homo sapiens |
| C335S/C3388S | the mutant shows kcat values that are similar to values observed with the single C335 mutant enzymes, Km values are largely unchanged compared to those of wild type BCATc, kcat/Km values are significantly lower than wild type values | Homo sapiens |
| C338S | the mutant enzyme exhibits kcat values for the BCAAs that were 50% lower than that observed with wild type enzyme. With the exception of L-glutamate, where the Km value is not changed and L-valine, where the Km value is 50% lower than the value for wild type BCATc, Km values for L-isoleucine and L-leucine are largely unaffected for the C338S mutant | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| on the basis of 5,5'-dithiobis(2-nitrobenzoic acid) titration of wild type BCATc, labeling of 6 thiol groups results in a loss of 60-70% of BCAT activity compared to that of the control enzyme incubated without 5,5'-dithiobis(2-nitrobenzoic acid) and assayed without dithiothreitol | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography and Mono-Q HR 5/5 column chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-isoleucine + 2-oxoglutarate | - |
Homo sapiens | 3-methyl-2-oxopentanoate + L-glutamate | - |
? |  |
| L-leucine + 2-oxoglutarate | - |
Homo sapiens | 2-oxoisohexanoate + L-glutamate | - |
? | |
| L-valine + 2-oxoglutarate | - |
Homo sapiens | 3-methyl-2-oxobutanoate + L-glutamate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BCATc | cytosolic isoform | Homo sapiens |
| branched-chain aminotransferase | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 141 | - |
L-glutamate | mutant enzyme C335S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 170 | - |
L-valine | mutant enzyme C335S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 208 | - |
L-glutamate | mutant enzyme C335S/C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 213 | - |
L-leucine | mutant enzyme C335S/C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 253 | - |
L-leucine | mutant enzyme C335S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 286 | - |
L-leucine | mutant enzyme C221S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 292 | - |
L-valine | mutant enzyme C335S/C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 341 | - |
L-isoleucine | mutant enzyme C335S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 403 | - |
L-glutamate | mutant enzyme C221S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 419 | - |
L-isoleucine | mutant enzyme C335S/C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 430 | - |
L-leucine | mutant enzyme C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 443 | - |
L-valine | mutant enzyme C221S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 503 | - |
L-isoleucine | mutant enzyme C221S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 517 | - |
L-glutamate | mutant enzyme C242S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 529 | - |
L-isoleucine | mutant enzyme C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 597 | - |
L-valine | mutant enzyme C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 651 | - |
L-leucine | mutant enzyme C242S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 688 | - |
L-valine | mutant enzyme C242S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 741 | - |
L-glutamate | mutant enzyme C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 756 | - |
L-leucine | mutant enzyme C235S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 779 | - |
L-isoleucine | mutant enzyme C235S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 803 | - |
L-leucine | mutant enzyme C293S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 838 | - |
L-valine | mutant enzyme C235S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 843 | - |
L-isoleucine | mutant enzyme C293S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 870 | - |
L-isoleucine | mutant enzyme C242S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 898 | - |
L-leucine | wild type enzyme, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 1035 | - |
L-glutamate | mutant enzyme C293S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 1075 | - |
L-glutamate | wild type enzyme, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 1075 | - |
L-isoleucine | wild type enzyme, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 1111 | - |
L-valine | mutant enzyme C293S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 1157 | - |
L-glutamate | mutant enzyme C235S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 1236 | - |
L-valine | wild type enzyme, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 31 | - |
L-glutamate | mutant enzyme C221S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 31 | - |
L-glutamate | mutant enzyme C335S/C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 40 | - |
L-glutamate | mutant enzyme C242S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 43 | - |
L-glutamate | mutant enzyme C335S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 62 | - |
L-glutamate | mutant enzyme C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 89 | - |
L-valine | mutant enzyme C335S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 103 | - |
L-glutamate | mutant enzyme C293S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 108 | - |
L-glutamate | wild type enzyme, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 126 | - |
L-glutamate | mutant enzyme C235S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 132 | - |
L-valine | mutant enzyme C335S/C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 201 | - |
L-valine | mutant enzyme C221S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 313 | - |
L-valine | mutant enzyme C242S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 474 | - |
L-leucine | mutant enzyme C335S/C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 505 | - |
L-leucine | mutant enzyme C335S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 579 | - |
L-valine | mutant enzyme C293S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 597 | - |
L-valine | mutant enzyme C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 618 | - |
L-valine | wild type enzyme, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 1059 | - |
L-leucine | mutant enzyme C221S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 1118 | - |
L-isoleucine | mutant enzyme C221S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 1302 | - |
L-leucine | mutant enzyme C242S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 1364 | - |
L-isoleucine | mutant enzyme C335S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 1397 | - |
L-valine | mutant enzyme C235S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 1496 | - |
L-isoleucine | mutant enzyme C335S/C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 1652 | - |
L-leucine | mutant enzyme C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 2071 | - |
L-isoleucine | mutant enzyme C242S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 2107 | - |
L-isoleucine | mutant enzyme C293S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 2116 | - |
L-isoleucine | mutant enzyme C338S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 2294 | - |
L-leucine | mutant enzyme C293S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 2494 | - |
L-leucine | wild type enzyme, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 3151 | - |
L-leucine | mutant enzyme C235S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 3359 | - |
L-isoleucine | wild type enzyme, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
| 3895 | - |
L-isoleucine | mutant enzyme C235S, in 25 mM potassium phosphate buffer at pH 7.8, at 37°C | Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
