Cloned (Comment) | Organism |
---|---|
ilvE gene of Escherichia coli is inserted into the region downstream of the tac-promotor, enzyme overproduced by about a 100fold in Escherichia coli W3110 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
2-oxoglutarate | pH 8.0, 25°C, substrate L-tyrosine | Escherichia coli | |
0.26 | - |
2-oxoglutarate | pH 8.0, 25°C, substrate L-phenylalanine | Escherichia coli | |
0.42 | - |
L-isoleucine | pH 8.0, 25°C, 2-oxoglutarate as amino group acceptor | Escherichia coli | |
0.56 | - |
2-oxoglutarate | pH 8.0, 25°C, substrate L-tryptophan | Escherichia coli | |
0.89 | - |
L-phenylalanine | pH 8.0, 25°C | Escherichia coli | |
1 | - |
2-oxoglutarate | pH 8.0, 25°C, substrate L-methionine | Escherichia coli | |
1.7 | - |
2-oxoglutarate | pH 8.0, 25°C, substrate L-valine | Escherichia coli | |
2.2 | - |
L-leucine | pH 8.0, 25°C | Escherichia coli | |
2.4 | - |
2-oxoglutarate | pH 8.0, 25°C, substrate L-isoleucine | Escherichia coli | |
2.7 | - |
L-valine | pH 8.0, 25°C | Escherichia coli | |
6.6 | - |
2-oxoglutarate | pH 8.0, 25°C, substrate L-leucine | Escherichia coli | |
7 | - |
L-tyrosine | pH 8.0, 25°C, 2-oxoglutarate as amino group acceptor | Escherichia coli | |
19 | - |
L-methionine | pH 8.0, 25°C, 2-oxoglutarate as amino group acceptor | Escherichia coli | |
72 | - |
L-tryptophan | pH 8.0, 25°C, 2-oxoglutarate as amino group acceptor | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
6 * 34000, SDS-PAGE | Escherichia coli |
195000 | - |
gel filtration combined with angle laser light scattering technique | Escherichia coli |
197000 | - |
sedimentation equilibrium | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | Escherichia coli | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-isoleucine + 2-oxoglutarate | Escherichia coli W3110 / ATCC 27325 | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | Escherichia coli | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | Escherichia coli W3110 / ATCC 27325 | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | Escherichia coli | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli W3110 / ATCC 27325 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23.9 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | - |
Escherichia coli | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-isoleucine + 2-oxoglutarate | - |
Escherichia coli W3110 / ATCC 27325 | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Escherichia coli | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Escherichia coli W3110 / ATCC 27325 | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-methionine + 2-oxoglutarate | - |
Escherichia coli | 4-methylsulfanyl-2-oxobutanoate + L-glutamate | - |
? | |
L-methionine + 2-oxoglutarate | - |
Escherichia coli W3110 / ATCC 27325 | 4-methylsulfanyl-2-oxobutanoate + L-glutamate | - |
? | |
L-phenylalanine + 2-oxoglutarate | - |
Escherichia coli | phenylpyruvate + L-glutamate | - |
r | |
L-phenylalanine + 2-oxoglutarate | - |
Escherichia coli W3110 / ATCC 27325 | phenylpyruvate + L-glutamate | - |
r | |
L-tryptophan + 2-oxoglutarate | - |
Escherichia coli | 2-oxo-3-indolylpropanoate + L-glutamate | - |
r | |
L-tyrosine + 2-oxoglutarate | - |
Escherichia coli | p-hydroxyphenylpyruvate + L-glutamate | - |
r | |
L-tyrosine + 2-oxoglutarate | - |
Escherichia coli W3110 / ATCC 27325 | p-hydroxyphenylpyruvate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Escherichia coli | 3-methyl-2-oxobutanoate + L-glutamate | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 34000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
BcaT | - |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | stable within 10 min | Escherichia coli |
6.5 | 9 | stable for 24 h at 25° | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |