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Literature summary for 2.6.1.42 extracted from

  • Inoue, K.; Kuramitsu, S.; Aki, K.; Watanabe, Y.; Takagi, T.; Nishigai, M.; Ikai, A.; Kagamiyama, H.
    Branched-chain amino acid aminotransferase of Escherichia coli: overproduction and properties (1988), J. Biochem., 104, 777-784.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
ilvE gene of Escherichia coli is inserted into the region downstream of the tac-promotor, enzyme overproduced by about a 100fold in Escherichia coli W3110 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
2-oxoglutarate pH 8.0, 25°C, substrate L-tyrosine Escherichia coli
0.26
-
2-oxoglutarate pH 8.0, 25°C, substrate L-phenylalanine Escherichia coli
0.42
-
L-isoleucine pH 8.0, 25°C, 2-oxoglutarate as amino group acceptor Escherichia coli
0.56
-
2-oxoglutarate pH 8.0, 25°C, substrate L-tryptophan Escherichia coli
0.89
-
L-phenylalanine pH 8.0, 25°C Escherichia coli
1
-
2-oxoglutarate pH 8.0, 25°C, substrate L-methionine Escherichia coli
1.7
-
2-oxoglutarate pH 8.0, 25°C, substrate L-valine Escherichia coli
2.2
-
L-leucine pH 8.0, 25°C Escherichia coli
2.4
-
2-oxoglutarate pH 8.0, 25°C, substrate L-isoleucine Escherichia coli
2.7
-
L-valine pH 8.0, 25°C Escherichia coli
6.6
-
2-oxoglutarate pH 8.0, 25°C, substrate L-leucine Escherichia coli
7
-
L-tyrosine pH 8.0, 25°C, 2-oxoglutarate as amino group acceptor Escherichia coli
19
-
L-methionine pH 8.0, 25°C, 2-oxoglutarate as amino group acceptor Escherichia coli
72
-
L-tryptophan pH 8.0, 25°C, 2-oxoglutarate as amino group acceptor Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
6 * 34000, SDS-PAGE Escherichia coli
195000
-
gel filtration combined with angle laser light scattering technique Escherichia coli
197000
-
sedimentation equilibrium Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-isoleucine + 2-oxoglutarate Escherichia coli
-
3-methyl-2-oxopentanoate + L-glutamate
-
r
L-isoleucine + 2-oxoglutarate Escherichia coli W3110 / ATCC 27325
-
3-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + 2-oxoglutarate Escherichia coli
-
4-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + 2-oxoglutarate Escherichia coli W3110 / ATCC 27325
-
4-methyl-2-oxopentanoate + L-glutamate
-
r
L-valine + 2-oxoglutarate Escherichia coli
-
3-methyl-2-oxobutanoate + L-glutamate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli W3110 / ATCC 27325
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.9
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + 2-oxoglutarate
-
Escherichia coli 3-methyl-2-oxopentanoate + L-glutamate
-
r
L-isoleucine + 2-oxoglutarate
-
Escherichia coli W3110 / ATCC 27325 3-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + 2-oxoglutarate
-
Escherichia coli 4-methyl-2-oxopentanoate + L-glutamate
-
r
L-leucine + 2-oxoglutarate
-
Escherichia coli W3110 / ATCC 27325 4-methyl-2-oxopentanoate + L-glutamate
-
r
L-methionine + 2-oxoglutarate
-
Escherichia coli 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
?
L-methionine + 2-oxoglutarate
-
Escherichia coli W3110 / ATCC 27325 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
?
L-phenylalanine + 2-oxoglutarate
-
Escherichia coli phenylpyruvate + L-glutamate
-
r
L-phenylalanine + 2-oxoglutarate
-
Escherichia coli W3110 / ATCC 27325 phenylpyruvate + L-glutamate
-
r
L-tryptophan + 2-oxoglutarate
-
Escherichia coli 2-oxo-3-indolylpropanoate + L-glutamate
-
r
L-tyrosine + 2-oxoglutarate
-
Escherichia coli p-hydroxyphenylpyruvate + L-glutamate
-
r
L-tyrosine + 2-oxoglutarate
-
Escherichia coli W3110 / ATCC 27325 p-hydroxyphenylpyruvate + L-glutamate
-
r
L-valine + 2-oxoglutarate
-
Escherichia coli 3-methyl-2-oxobutanoate + L-glutamate
-
r

Subunits

Subunits Comment Organism
hexamer 6 * 34000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
BcaT
-
Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 stable within 10 min Escherichia coli
6.5 9 stable for 24 h at 25° Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Escherichia coli