Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-Azauracil | weak, kinetics, not reversible by pyridoxal phosphate | Rattus norvegicus | |
6-azauridine | weak | Rattus norvegicus | |
Amino-oxyacetate | strong | Rattus norvegicus | |
D-cycloserine | - |
Rattus norvegicus | |
gabaculine | - |
Rattus norvegicus | |
additional information | no inhibitor: dihydrothymine, dihydrouracil, N-carbamoyl-beta-alanine, N-carbamoyl-DL-3-aminoisobutyrate; no inhibitor: precursors of beta-alanine or (R)-3-amino-2-methylpropanoate, i.e. orotic acid, uracil, thymine | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
pyruvate | pH 8.8, 37°C | Rattus norvegicus | |
0.81 | - |
beta-Alanine | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
4 * 52000, SDS-PAGE | Rattus norvegicus |
220000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
additional information | distribution in rat tissue | Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
4°C, 0.05 mg protein/ml, in 0.1 M potassium phosphate buffer, pH 7, 1 mM EDTA, 2 mM 2-mercaptoethanol, about 50% loss of activity within a few days, 80% loss within 1 week | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-amino-2-methylpropanoate + glyoxylate | - |
Rattus norvegicus | 2-methyl-3-oxopropanoate + glycine | - |
? | |
(R)-3-amino-2-methylpropanoate + oxaloacetate | - |
Rattus norvegicus | 2-methyl-3-oxopropanoate + L-aspartate | - |
? | |
(R)-3-amino-2-methylpropanoate + pyruvate | - |
Rattus norvegicus | 2-methyl-3-oxopropanoate + L-alanine | - |
? | |
5-aminolevulinic acid + pyruvate | - |
Rattus norvegicus | 5-oxolevulinic acid + L-alanine | - |
? | |
beta-alanine + pyruvate | - |
Rattus norvegicus | malonic semialdehyde + L-alanine | - |
? | |
additional information | overview | Rattus norvegicus | ? | - |
? | |
additional information | no substrate: 2-oxoglutarate | Rattus norvegicus | ? | - |
? | |
additional information | no substrate: phenylpyruvate | Rattus norvegicus | ? | - |
? | |
NG,NG'-dimethyl-(S)-arginine + pyruvate | - |
Rattus norvegicus | ? + L-alanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 52000, SDS-PAGE | Rattus norvegicus |
tetramer | cross-linking experiments: tetramer composed of two dimers | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 6.7 | Rattus norvegicus |
9.5 | - |
- |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Rattus norvegicus |