Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-fluoro-beta-alanine | - |
Rattus norvegicus | |
gabaculine | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
4 * 52000, SDS-PAGE | Rattus norvegicus |
220000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay principle | Rattus norvegicus |
11.3 | - |
pH 8.8, 37°C | Rattus norvegicus |
Storage Stability | Organism |
---|---|
4°C, 0.05 mg protein/ml, in 0.1 M potassium phosphate buffer, pH 7, 1 mM EDTA, 2 mM 2-mercaptoethanol, about 50% loss of activity within a few days, 80% loss within 1 week | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-amino-2-methylpropanoate + glyoxylate | with 90% efficiency compared to pyruvate | Rattus norvegicus | 2-methyl-3-oxopropanoate + glycine | - |
r | |
(R)-3-amino-2-methylpropanoate + oxaloacetate | with 63% efficiency compared to pyruvate | Rattus norvegicus | 2-methyl-3-oxopropanoate + L-aspartate | - |
r | |
beta-alanine + pyruvate | transamination at 66% the rate of (R)-3-amino-2-methylpropanoate | Rattus norvegicus | malonic semialdehyde + L-alanine | - |
? | |
additional information | overview | Rattus norvegicus | ? | - |
? | |
additional information | no substrate: 2-oxoglutarate | Rattus norvegicus | ? | - |
? | |
additional information | gamma-aminobutanoate is neither substrate nor inhibitor | Rattus norvegicus | ? | - |
? | |
additional information | no substrate: phenylpyruvate | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 52000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | gamma-aminobutanoate is neither substrate nor inhibitor | Rattus norvegicus |