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Literature summary for 2.6.1.4 extracted from

  • Zhang, Z.; Mao, X.; Ou, J.; Ye, N.; Zhang, J.; Peng, X.
    Distinct photorespiratory reactions are preferentially catalyzed by glutamate:glyoxylate and serine:glyoxylate aminotransferases in rice (2015), J. Photochem. Photobiol. B, 142, 110-117.
    View publication on PubMed
Search for more literature for 2.6.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in yeast Oryza sativa Japonica Group

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 glyoxylate cosubstrate L-glutamate, pH 7.6, 30°C Oryza sativa Japonica Group
0.32
-
 glyoxylate cosubstrate L-alanine, pH 7.6, 30°C Oryza sativa Japonica Group
0.55
-
 2-oxoglutarate cosubstrate L-alanine, pH 7.6, 30°C Oryza sativa Japonica Group
1.57
-
 L-glutamate cosubstrate glyoxylate, pH 7.6, 30°C Oryza sativa Japonica Group
2.37
-
 L-alanine cosubstrate glyoxylate, pH 7.6, 30°C Oryza sativa Japonica Group
2.96
-
 L-alanine cosubstrate 2-oxoglutarate, pH 7.6, 30°C Oryza sativa Japonica Group

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 4 * 54000, SDS-PAGE Oryza sativa Japonica Group
240000
-
 PAGE Oryza sativa Japonica Group

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group A0A0P0X1V6
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + 2-oxoglutarate
-
 Oryza sativa Japonica Group pyruvate + L-glutamate
-
 ?
L-alanine + glyoxylate
-
 Oryza sativa Japonica Group pyruvate + glycine
-
 ?
L-glutamate + glyoxylate
-
 Oryza sativa Japonica Group 2-oxoglutarate + glycine
-
 ?
L-glutamate + pyruvate
-
 Oryza sativa Japonica Group 2-oxoglutarate + L-alanine
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 54000, SDS-PAGE Oryza sativa Japonica Group

Synonyms

Synonyms Comment Organism
Os07g0108300
-
 Oryza sativa Japonica Group

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
132.9
-
 L-alanine cosubstrate glyoxylate, pH 7.6, 30°C Oryza sativa Japonica Group
140.6
-
 L-alanine cosubstrate 2-oxoglutarate, pH 7.6, 30°C Oryza sativa Japonica Group
144.9
-
 L-glutamate cosubstrate glyoxylate, pH 7.6, 30°C Oryza sativa Japonica Group

General Information

General Information Comment Organism
physiological function rice leaves usually show 3-4 times higher abundance of glutamate relative to serine, implicating that glutamate:glyoxylate aminotransferase GGAT may preferentially utilize glyoxylate to form glycine over serine:glyoxylate aminotransferase SGAT. When SGAT or GGAT activity is regulated by gene transformation or nitrogen deficiency, respectively, the glycine content is positively related to GGAT activities, while both serine and glycine contents are negatively related to SGAT activities, suggesting that GGAT preferentially catalyzes the conversion of glyoxylate into glycine while SGAT is mainly responsible for the transamination reaction of serine to hydroxypyruvate in the photorespiratory pathway of rice Oryza sativa Japonica Group