Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ammonium chloride | 50% inhibition at 15 mM | Rhodopseudomonas palustris | |
glycine | weak, 30% inhibition at 15 mM | Rhodopseudomonas palustris | |
L-serine | weak, 20% inhibition at 15 mM | Rhodopseudomonas palustris | |
additional information | no inhibition by aspartate; no substrate inhibition | Rhodopseudomonas palustris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.75 | - |
glyoxylate | pH 7.5, 30°C | Rhodopseudomonas palustris | |
6.2 | - |
L-glutamate | pH 7.5, 30°C | Rhodopseudomonas palustris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
1 * 42000, SDS-PAGE | Rhodopseudomonas palustris |
45500 | - |
gel filtration | Rhodopseudomonas palustris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + L-glutamate | Rhodopseudomonas palustris | involved in the supply of glycine for extracellular porphyrin production | glycine + 2-oxoglutarate | - |
? | |
glyoxylate + L-glutamate | Rhodopseudomonas palustris No. 7 | involved in the supply of glycine for extracellular porphyrin production | glycine + 2-oxoglutarate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodopseudomonas palustris | - |
No. 7 | - |
Rhodopseudomonas palustris No. 7 | - |
No. 7 | - |
Purification (Comment) | Organism |
---|---|
191fold to homogeneity | Rhodopseudomonas palustris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | activity depends on growth phase | Rhodopseudomonas palustris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.25 | - |
purified enzyme | Rhodopseudomonas palustris |
Storage Stability | Organism |
---|---|
-20°C, fairly stable for 8 months | Rhodopseudomonas palustris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + L-glutamate | highly specific for glyoxylate and L-glutamate as substrates | Rhodopseudomonas palustris | glycine + 2-oxoglutarate | - |
? | |
glyoxylate + L-glutamate | involved in the supply of glycine for extracellular porphyrin production | Rhodopseudomonas palustris | glycine + 2-oxoglutarate | - |
? | |
glyoxylate + L-glutamate | highly specific for glyoxylate and L-glutamate as substrates | Rhodopseudomonas palustris No. 7 | glycine + 2-oxoglutarate | - |
? | |
glyoxylate + L-glutamate | involved in the supply of glycine for extracellular porphyrin production | Rhodopseudomonas palustris No. 7 | glycine + 2-oxoglutarate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42000, SDS-PAGE | Rhodopseudomonas palustris |
Synonyms | Comment | Organism |
---|---|---|
GlyAT | - |
Rhodopseudomonas palustris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Rhodopseudomonas palustris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
inactivation | Rhodopseudomonas palustris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
46.8 | - |
L-glutamate | pH 7.5, 30°C | Rhodopseudomonas palustris | |
48.2 | - |
glyoxylate | pH 7.5, 30°C | Rhodopseudomonas palustris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rhodopseudomonas palustris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | 80% activity at pH 5.0 and pH 7.5 | Rhodopseudomonas palustris |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
inactivation | Rhodopseudomonas palustris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Rhodopseudomonas palustris |