Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | 50% inhibition at 5 mM | Rattus norvegicus | |
Isonicotinic acid hydrazide | glyoxylate partially protects | Rattus norvegicus | |
Maleic acid | weak | Rattus norvegicus | |
PCMB | no inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
glutamate | pH 7.3 | Rattus norvegicus | |
8.3 | - |
glyoxylate | pH 7.3 | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + L-glutamate | Rattus norvegicus | - |
glycine + 2-oxoglutarate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, about 30fold | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | limited activity | Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
additional information | tissue distribution, very low activity in lung, testes or diaphragm | Rattus norvegicus | - |
spleen | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.13 | - |
partially purified enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + L-alanine | no activity | Rattus norvegicus | glycine + pyruvate | - |
? | |
glyoxylate + L-glutamate | - |
Rattus norvegicus | glycine + 2-oxoglutarate | - |
ir | |
additional information | substrate specificity | Rattus norvegicus | ? | - |
? | |
additional information | no substrates are D-amino acid isomers | Rattus norvegicus | ? | - |
? | |
additional information | no activity with ammonia, glutathione, L-ornithine or 4-aminobutanoate | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutamic-glycine transaminase | - |
Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
5 min, stable | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8.2 | about half-maximal activity at pH 6.2 and pH 8.2 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | addition to enzyme system mostly without effect | Rattus norvegicus |