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Literature summary for 2.6.1.39 extracted from

  • Karsten, W.E.; Reyes, Z.L.; Bobyk, K.D.; Cook, P.F.; Chooback, L.
    Mechanism of the aromatic aminotransferase encoded by the Aro8 gene from Saccharomyces cerevisiae (2011), Arch. Biochem. Biophys., 516, 67-74.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged protein expressed in Escherichia coli BL21-Codon-Plus(DE3)-RIL Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
2-oxoadipate pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae
0.5
-
L-tyrosine pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae
1
-
L-phenylalanine pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae
3.6
-
2-oxoglutarate pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae
8.8
-
L-2-aminoadipate pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae
26
-
L-glutamate pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56168
-
2 * 56168 Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+) Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + L-2-aminoadipate
-
Saccharomyces cerevisiae 2-oxoadipate + L-glutamate
-
r
2-oxoglutarate + L-tyrosine
-
Saccharomyces cerevisiae L-glutamate + 4-hydroxyphenylpyruvate
-
r
L-2-aminoadipate + 4-hydroxyphenylpyruvate
-
Saccharomyces cerevisiae 2-oxoadipate + L-tyrosine
-
?
L-glutamate + 4-hydroxyphenylpyruvate
-
Saccharomyces cerevisiae 2-oxoglutarate + L-tyrosine
-
r
L-phenylalanine + 2-oxoglutarate
-
Saccharomyces cerevisiae phenylpyruvate + L-glutamate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 56168 Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
alpha-aminoadipate aminotransferase (gene aro8)
-
Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.3
-
L-phenylalanine pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae
12.3
-
L-tyrosine pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae
12.8
-
L-glutamate pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae
18.4
-
L-2-aminoadipate pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.6 The V/Et and V/Kalpha-ketoadipateEt pH profiles are pH independent from pH 6.5 to 9.6, while the V/KL-tyrosine pH-rate profile decreases below a single pKa of 7.0 ± 0.1 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Saccharomyces cerevisiae