Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli BL21-Codon-Plus(DE3)-RIL | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
2-oxoadipate | pH 7.0, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.5 | - |
L-tyrosine | pH 7.0, temperature not specified in the publication | Saccharomyces cerevisiae | |
1 | - |
L-phenylalanine | pH 7.0, temperature not specified in the publication | Saccharomyces cerevisiae | |
3.6 | - |
2-oxoglutarate | pH 7.0, temperature not specified in the publication | Saccharomyces cerevisiae | |
8.8 | - |
L-2-aminoadipate | pH 7.0, temperature not specified in the publication | Saccharomyces cerevisiae | |
26 | - |
L-glutamate | pH 7.0, temperature not specified in the publication | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56168 | - |
2 * 56168 | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + L-2-aminoadipate | - |
Saccharomyces cerevisiae | 2-oxoadipate + L-glutamate | - |
r | |
2-oxoglutarate + L-tyrosine | - |
Saccharomyces cerevisiae | L-glutamate + 4-hydroxyphenylpyruvate | - |
r | |
L-2-aminoadipate + 4-hydroxyphenylpyruvate | - |
Saccharomyces cerevisiae | 2-oxoadipate + L-tyrosine | - |
? | |
L-glutamate + 4-hydroxyphenylpyruvate | - |
Saccharomyces cerevisiae | 2-oxoglutarate + L-tyrosine | - |
r | |
L-phenylalanine + 2-oxoglutarate | - |
Saccharomyces cerevisiae | phenylpyruvate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 56168 | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
alpha-aminoadipate aminotransferase (gene aro8) | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.3 | - |
L-phenylalanine | pH 7.0, temperature not specified in the publication | Saccharomyces cerevisiae | |
12.3 | - |
L-tyrosine | pH 7.0, temperature not specified in the publication | Saccharomyces cerevisiae | |
12.8 | - |
L-glutamate | pH 7.0, temperature not specified in the publication | Saccharomyces cerevisiae | |
18.4 | - |
L-2-aminoadipate | pH 7.0, temperature not specified in the publication | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.6 | The V/Et and V/Kalpha-ketoadipateEt pH profiles are pH independent from pH 6.5 to 9.6, while the V/KL-tyrosine pH-rate profile decreases below a single pKa of 7.0 ± 0.1 | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Saccharomyces cerevisiae |