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Literature summary for 2.6.1.39 extracted from

  • Tomita, T.; Miyagawa, T.; Miyazaki, T.; Fushinobu, S.; Kuzuyama, T.; Nishiyama, M.
    Mechanism for multiple-substrates recognition of alpha-aminoadipate aminotransferase from Thermus thermophilus (2008), Proteins, 75, 348-359.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of AAA-AT in four forms: with pyridoxal 5'-phosphate (PLP) (PLP complex), with PLP and leucine (PLP/Leu complex), with N-phosphopyridoxyl-leucine (PPL) (PPL complex), and with N-phosphopyridoxyl-alpha-aminoadipate (PPA) at 2.67, 2.26, 1.75, and 1.67 A resolution, respectively Thermus thermophilus
in complex with pyridoxal 5'-phosphate, with pyridoxal 5'-phosphate and leucine, with N-phosphopyridoxyl-leucine, and with N-phosphopyridoxyl-alpha-aminoadipate, at 2.67, 2.26, 1.75 and 1.67 A resolution, respectively. Enzyme contains a mobile alpha2-helix involved in substrate recognition Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminoadipate + pyruvate
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Thermus thermophilus 2-oxoadipate + L-alanine
-
?
2-oxoadipate + L-glutamate
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Thermus thermophilus L-2-aminoadipate + 2-oxoglutarate
-
?
L-leucine + 2-oxoglutarate
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Thermus thermophilus 4-methyl-2-oxopentanoate + L-glutamate
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?
additional information AAA-AT can recognize various kinds of substrates using the mobile alpha2 helix Thermus thermophilus ?
-
?
phenylpyruvate + 2-aminoadipate
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Thermus thermophilus L-phenylalanine + 2-oxoglutarate
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?

Synonyms

Synonyms Comment Organism
AAA-AT
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Thermus thermophilus
alpha-aminoadipate aminotransferase
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Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
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the crystal structures and site-directed mutagenesis reveales that intersubunit-electrostatic interactions contributes to the elevated thermostability of this enzyme Thermus thermophilus