Crystallization (Comment) | Organism |
---|---|
strcutures of mutants T330S, E243A, N328A, to 2.7-1.95 A resolution | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
E243A | mutation in acive-site residue. Mutant retains activity, but binds alpha-ketoglutarate in a different conformation | Mycobacterium tuberculosis |
N328A | mutation in acive-site residue. Mutant binds pyridoxal 5'-phospate but is inactive | Mycobacterium tuberculosis |
T330A | mutation in acive-site residue, mutant cannot bind pyridoxal 5'-phospate and is inactive | Mycobacterium tuberculosis |
T330S | mutation in acive-site residue, mutant cannot bind pyridoxal 5'-phospate and is inactive | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
L-lysine | wild-type, pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
1.6 | - |
2-oxoglutarate | mutant E243A, pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
1.7 | - |
L-lysine | mutant E243A, pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
2.3 | - |
2-oxoglutarate | wild-type, pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WQ77 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | P9WQ77 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate | - |
Mycobacterium tuberculosis | (S)-2-amino-6-oxohexanoate + L-glutamate | - |
? | |
L-lysine + 2-oxoglutarate | - |
Mycobacterium tuberculosis ATCC 25618 | (S)-2-amino-6-oxohexanoate + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LAT | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.92 | - |
L-lysine | wild-type, pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
1 | - |
2-oxoglutarate | mutant E243A, pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
1.17 | - |
L-lysine | mutant E243A, pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis | |
1.43 | - |
2-oxoglutarate | wild-type, pH not specified in the publication, temperature not specified in the publication | Mycobacterium tuberculosis |