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Literature summary for 2.6.1.36 extracted from

  • Tripathi, S.; Agarwal, A.; Ramachandran, R.
    Mutational analysis of Mycobacterium tuberculosis lysine epsilon-aminotransferase and inhibitor co-crystal structures, reveals distinct binding modes (2015), Biochem. Biophys. Res. Commun., 463, 154-160.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
strcutures of mutants T330S, E243A, N328A, to 2.7-1.95 A resolution Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
E243A mutation in acive-site residue. Mutant retains activity, but binds alpha-ketoglutarate in a different conformation Mycobacterium tuberculosis
N328A mutation in acive-site residue. Mutant binds pyridoxal 5'-phospate but is inactive Mycobacterium tuberculosis
T330A mutation in acive-site residue, mutant cannot bind pyridoxal 5'-phospate and is inactive Mycobacterium tuberculosis
T330S mutation in acive-site residue, mutant cannot bind pyridoxal 5'-phospate and is inactive Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
L-lysine wild-type, pH not specified in the publication, temperature not specified in the publication Mycobacterium tuberculosis
1.6
-
2-oxoglutarate mutant E243A, pH not specified in the publication, temperature not specified in the publication Mycobacterium tuberculosis
1.7
-
L-lysine mutant E243A, pH not specified in the publication, temperature not specified in the publication Mycobacterium tuberculosis
2.3
-
2-oxoglutarate wild-type, pH not specified in the publication, temperature not specified in the publication Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WQ77
-
-
Mycobacterium tuberculosis ATCC 25618 P9WQ77
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + 2-oxoglutarate
-
Mycobacterium tuberculosis (S)-2-amino-6-oxohexanoate + L-glutamate
-
?
L-lysine + 2-oxoglutarate
-
Mycobacterium tuberculosis ATCC 25618 (S)-2-amino-6-oxohexanoate + L-glutamate
-
?

Synonyms

Synonyms Comment Organism
LAT
-
Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.92
-
L-lysine wild-type, pH not specified in the publication, temperature not specified in the publication Mycobacterium tuberculosis
1
-
2-oxoglutarate mutant E243A, pH not specified in the publication, temperature not specified in the publication Mycobacterium tuberculosis
1.17
-
L-lysine mutant E243A, pH not specified in the publication, temperature not specified in the publication Mycobacterium tuberculosis
1.43
-
2-oxoglutarate wild-type, pH not specified in the publication, temperature not specified in the publication Mycobacterium tuberculosis