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Literature summary for 2.6.1.35 extracted from
- Formation of glycine from glyoxylate in Micrococcus denitrificans (1966), Biochem. J., 99, 27P-28P.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| erythro-L-hydroxyaspartate | competitive versus L-aspartate and L-serine | Paracoccus denitrificans |  |
| L-serine | competitive versus L-aspartate | Paracoccus denitrificans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
glyoxylate | pH 7.1, 25°C, with substrate L-aspartate | Paracoccus denitrificans | |
| 1.9 | - |
L-aspartate | pH 7.1, 25°C | Paracoccus denitrificans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no metal ion requirement | Paracoccus denitrificans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + L-aspartate | Paracoccus denitrificans | key reaction in 3-hydroxy-aspartate pathway of glyoxylate metabolism | glycine + oxaloacetate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paracoccus denitrificans | - |
inducible | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, 40fold | Paracoccus denitrificans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.043 | - |
partially purified enzyme | Paracoccus denitrificans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 2°C, partially purified enzyme, pH 7.0, quite stable | Paracoccus denitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + L-aspartate | reverse reaction: specific for glyoxylate as amino acceptor | Paracoccus denitrificans | glycine + oxaloacetate | - |
r | |
| glyoxylate + L-aspartate | poor substrates are asparagine or threo-L-beta-hydroxyaspartate | Paracoccus denitrificans | glycine + oxaloacetate | - |
r | |
| glyoxylate + L-aspartate | favoured reaction, equilibrium in direction of glycine formation | Paracoccus denitrificans | glycine + oxaloacetate | - |
r | |
| glyoxylate + L-aspartate | key reaction in 3-hydroxy-aspartate pathway of glyoxylate metabolism | Paracoccus denitrificans | glycine + oxaloacetate | - |
r | |
| glyoxylate + L-serine | transamination at about 30% the rate of L-aspartate | Paracoccus denitrificans | hydroxypyruvate + glycine | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 2 | - |
quite stable at pH 7.0 | Paracoccus denitrificans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | - |
- |
Paracoccus denitrificans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
quite stable at 2°C | Paracoccus denitrificans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | requirement | Paracoccus denitrificans | |
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Release 2023.1 (January 2023)
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