BRENDA - Enzyme Database show
show all sequences of 2.6.1.35

Formation of glycine from glyoxylate in Micrococcus denitrificans

Gibbs, R.G.; Morris, J.G.; Biochem. J. 99, 27P-28P (1966)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
erythro-L-hydroxyaspartate
competitive versus L-aspartate and L-serine
Paracoccus denitrificans
L-serine
competitive versus L-aspartate
Paracoccus denitrificans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.43
-
glyoxylate
pH 7.1, 25C, with substrate L-aspartate
Paracoccus denitrificans
1.9
-
L-aspartate
pH 7.1, 25C
Paracoccus denitrificans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ion requirement
Paracoccus denitrificans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glyoxylate + L-aspartate
Paracoccus denitrificans
key reaction in 3-hydroxy-aspartate pathway of glyoxylate metabolism
glycine + oxaloacetate
-
Paracoccus denitrificans
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Paracoccus denitrificans
-
inducible
-
Purification (Commentary)
Commentary
Organism
partial, 40fold
Paracoccus denitrificans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.043
-
partially purified enzyme
Paracoccus denitrificans
Storage Stability
Storage Stability
Organism
2C, partially purified enzyme, pH 7.0, quite stable
Paracoccus denitrificans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + L-aspartate
reverse reaction: specific for glyoxylate as amino acceptor
639966
Paracoccus denitrificans
glycine + oxaloacetate
-
639966
Paracoccus denitrificans
r
glyoxylate + L-aspartate
poor substrates are asparagine or threo-L-beta-hydroxyaspartate
639966
Paracoccus denitrificans
glycine + oxaloacetate
-
639966
Paracoccus denitrificans
r
glyoxylate + L-aspartate
favoured reaction, equilibrium in direction of glycine formation
639966
Paracoccus denitrificans
glycine + oxaloacetate
-
639966
Paracoccus denitrificans
r
glyoxylate + L-aspartate
key reaction in 3-hydroxy-aspartate pathway of glyoxylate metabolism
639966
Paracoccus denitrificans
glycine + oxaloacetate
-
639966
Paracoccus denitrificans
r
glyoxylate + L-serine
transamination at about 30% the rate of L-aspartate
639966
Paracoccus denitrificans
hydroxypyruvate + glycine
-
639966
Paracoccus denitrificans
r
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2
-
quite stable at pH 7.0
Paracoccus denitrificans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.1
-
-
Paracoccus denitrificans
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
-
quite stable at 2C
Paracoccus denitrificans
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
requirement
Paracoccus denitrificans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
requirement
Paracoccus denitrificans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
erythro-L-hydroxyaspartate
competitive versus L-aspartate and L-serine
Paracoccus denitrificans
L-serine
competitive versus L-aspartate
Paracoccus denitrificans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.43
-
glyoxylate
pH 7.1, 25C, with substrate L-aspartate
Paracoccus denitrificans
1.9
-
L-aspartate
pH 7.1, 25C
Paracoccus denitrificans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ion requirement
Paracoccus denitrificans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glyoxylate + L-aspartate
Paracoccus denitrificans
key reaction in 3-hydroxy-aspartate pathway of glyoxylate metabolism
glycine + oxaloacetate
-
Paracoccus denitrificans
r
Purification (Commentary) (protein specific)
Commentary
Organism
partial, 40fold
Paracoccus denitrificans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.043
-
partially purified enzyme
Paracoccus denitrificans
Storage Stability (protein specific)
Storage Stability
Organism
2C, partially purified enzyme, pH 7.0, quite stable
Paracoccus denitrificans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + L-aspartate
reverse reaction: specific for glyoxylate as amino acceptor
639966
Paracoccus denitrificans
glycine + oxaloacetate
-
639966
Paracoccus denitrificans
r
glyoxylate + L-aspartate
poor substrates are asparagine or threo-L-beta-hydroxyaspartate
639966
Paracoccus denitrificans
glycine + oxaloacetate
-
639966
Paracoccus denitrificans
r
glyoxylate + L-aspartate
favoured reaction, equilibrium in direction of glycine formation
639966
Paracoccus denitrificans
glycine + oxaloacetate
-
639966
Paracoccus denitrificans
r
glyoxylate + L-aspartate
key reaction in 3-hydroxy-aspartate pathway of glyoxylate metabolism
639966
Paracoccus denitrificans
glycine + oxaloacetate
-
639966
Paracoccus denitrificans
r
glyoxylate + L-serine
transamination at about 30% the rate of L-aspartate
639966
Paracoccus denitrificans
hydroxypyruvate + glycine
-
639966
Paracoccus denitrificans
r
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2
-
quite stable at pH 7.0
Paracoccus denitrificans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.1
-
-
Paracoccus denitrificans
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
-
quite stable at 2C
Paracoccus denitrificans
Other publictions for EC 2.6.1.35
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
639968
Caballero
-
Regulation of reduced nitrogen ...
Rhodobacter capsulatus, Rhodobacter capsulatus E1F1
Arch. Microbiol.
152
508-511
1989
-
-
-
-
-
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
639967
Herbert
Asparagine and glutamine metab ...
Rhodoblastus acidophilus 7050 / DSM 137, Rhodoblastus acidophilus
Arch. Microbiol.
128
233-238
1980
2
-
-
-
-
-
1
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
639966
Gibbs
-
Formation of glycine from glyo ...
Paracoccus denitrificans
Biochem. J.
99
27P-28P
1966
-
-
-
-
-
-
2
2
-
1
-
1
-
1
-
-
1
-
-
-
1
1
5
-
-
-
1
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
2
-
2
-
1
-
1
-
-
-
1
-
-
1
1
5
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-