BRENDA - Enzyme Database show
show all sequences of 2.6.1.34

Functional characterization of dehydratase/aminotransferase pairs from Helicobacter and Campylobacter: enzymes distinguishing the pseudaminic acid and bacillosamine biosynthetic pathways

Schoenhofen, I.C.; McNally, D.J.; Vinogradov, E.; Whitfield, D.; Young, N.M.; Dick, S.; Wakarchuk, W.W.; Brisson, J.R.; Logan, S.M.; J. Biol. Chem. 281, 723-732 (2006)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.048
-
UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose
pH 7.7, 37C
Campylobacter jejuni subsp. jejuni
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + L-glutamate
Campylobacter jejuni subsp. jejuni
the enzyme (Cj1121c) is involved in biosynthesis of bacillosamine
UDP-4-amino-4,6-dideoxy-alpha-D-N-acetyl-D-glucosamine + 2-oxoglutarate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Campylobacter jejuni subsp. jejuni
Q0P9D3
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + L-glutamate
the enzyme (Cj1121c) is involved in biosynthesis of bacillosamine
692984
Campylobacter jejuni subsp. jejuni
UDP-4-amino-4,6-dideoxy-alpha-D-N-acetyl-D-glucosamine + 2-oxoglutarate
-
-
-
?
UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + L-glutamate
the enzyme (Cj1121c) does not utilize UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose as substrate
692984
Campylobacter jejuni subsp. jejuni
UDP-4-amino-4,6-dideoxy-alpha-D-N-acetyl-D-glucosamine + 2-oxoglutarate
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Campylobacter jejuni subsp. jejuni
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4
-
UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose
pH 7.7, 37C
Campylobacter jejuni subsp. jejuni
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
assay at
Campylobacter jejuni subsp. jejuni
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
a pyridoxal 5'-phosphate protein
Campylobacter jejuni subsp. jejuni
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
a pyridoxal 5'-phosphate protein
Campylobacter jejuni subsp. jejuni
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.048
-
UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose
pH 7.7, 37C
Campylobacter jejuni subsp. jejuni
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + L-glutamate
Campylobacter jejuni subsp. jejuni
the enzyme (Cj1121c) is involved in biosynthesis of bacillosamine
UDP-4-amino-4,6-dideoxy-alpha-D-N-acetyl-D-glucosamine + 2-oxoglutarate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + L-glutamate
the enzyme (Cj1121c) is involved in biosynthesis of bacillosamine
692984
Campylobacter jejuni subsp. jejuni
UDP-4-amino-4,6-dideoxy-alpha-D-N-acetyl-D-glucosamine + 2-oxoglutarate
-
-
-
?
UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + L-glutamate
the enzyme (Cj1121c) does not utilize UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose as substrate
692984
Campylobacter jejuni subsp. jejuni
UDP-4-amino-4,6-dideoxy-alpha-D-N-acetyl-D-glucosamine + 2-oxoglutarate
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Campylobacter jejuni subsp. jejuni
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4
-
UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose
pH 7.7, 37C
Campylobacter jejuni subsp. jejuni
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
assay at
Campylobacter jejuni subsp. jejuni
Other publictions for EC 2.6.1.34
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739604
Riegert
Structure of the external aldi ...
Campylobacter jejuni subsp. jejuni, Campylobacter jejuni subsp. jejuni ATCC 700819
Protein Sci.
24
1609-1616
2015
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714453
Morrison
Development of a multicomponen ...
Campylobacter jejuni
Bioorg. Med. Chem.
18
8167-8171
2010
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1
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692984
Schoenhofen
Functional characterization of ...
Campylobacter jejuni subsp. jejuni
J. Biol. Chem.
281
723-732
2006
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1
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1
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5
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1
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1
1
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714160
Olivier
In vitro biosynthesis of UDP-N ...
Campylobacter jejuni
Biochemistry
45
13659-13669
2006
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1
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639965
Distler
Enzymic synthesis of a diamino ...
Streptococcus pneumoniae
Arch. Biochem. Biophys.
116
466-478
1966
2
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1
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