BRENDA - Enzyme Database show
show all sequences of 2.6.1.32

Isolation of a mutant auxotrophic for L-alanine and identification of three major aminotransferases that synthesize L-alanine in Escherichia coli

Yoneyama, H.; Hori, H.; Lim, S.J.; Murata, T.; Ando, T.; Isogai, E.; Katsumata, R.; Biosci. Biotechnol. Biochem. 75, 930-938 (2011)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
C6UMN8
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Escherichia coli
D2AFZ3
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-
Expression
Organism
Commentary
Expression
Escherichia coli
level of yfdZ expression in minimal medium is higher than in rich medium
up
General Information
General Information
Commentary
Organism
physiological function
an alanine auxotroph (mutated in yfdZ) is isolated from a prototrophic double mutant deficient in AvtA and YfbQ, a novel alanine aminotransferase, by chemical mutagenesis; Escherichia coli synthesizes L-alanine by means of three aminotransferases, YfbQ, YfdZ, and AvtA; level of yfdZ expression in minimal medium is higher than in rich medium; an alanine auxotroph (mutated in yfdZ) is isolated from a prototrophic double mutant deficient in AvtA and YfbQ by chemical mutagenesis; Escherichia coli synthesizes L-alanine by means of three aminotransferases, YfbQ, YfdZ, and AvtA; YfbQ contributes to most of the alanine aminotransferase activity in rich medium
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
physiological function
an alanine auxotroph (mutated in yfdZ) is isolated from a prototrophic double mutant deficient in AvtA and YfbQ, a novel alanine aminotransferase, by chemical mutagenesis; Escherichia coli synthesizes L-alanine by means of three aminotransferases, YfbQ, YfdZ, and AvtA; level of yfdZ expression in minimal medium is higher than in rich medium
Escherichia coli
physiological function
an alanine auxotroph (mutated in yfdZ) is isolated from a prototrophic double mutant deficient in AvtA and YfbQ by chemical mutagenesis; Escherichia coli synthesizes L-alanine by means of three aminotransferases, YfbQ, YfdZ, and AvtA; YfbQ contributes to most of the alanine aminotransferase activity in rich medium
Escherichia coli
Expression (protein specific)
Organism
Commentary
Expression
Escherichia coli
level of yfdZ expression in minimal medium is higher than in rich medium
up
Other publictions for EC 2.6.1.32
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721875
Yoneyama
Isolation of a mutant auxotrop ...
Escherichia coli
Biosci. Biotechnol. Biochem.
75
930-938
2011
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639963
Kagan
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Separation of valine-glutamic ...
Pisum sativum
Dokl. Akad. Nauk SSSR
175
1171-1174
1967
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