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Literature summary for 2.6.1.31 extracted from
- Pyridoxamine-oxaloacetic transaminase of rat kidney (1964), J. Biol. Chem., 239, 1492-1497.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| arsenate | activation, less effective than phosphate | Rattus norvegicus |  |
| D-Aspartate | increases activity | Rattus norvegicus | |
| diphosphate | activation, less effective than phosphate | Rattus norvegicus | |
| phosphate | essential, activation in absence of diethylstilbestrol and pyridoxal 5'-phosphate | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | inhibition at pH 7.0 is higher than at pH 8.5, 3.33 mM in presence of equimolar concentrations of oxaloacetate | Rattus norvegicus | |
| 2-Oxomalonate | inhibition at pH 7.0 is higher than at pH 8.5, 0.4 mM in presence of equimolar concentrations of oxaloacetate | Rattus norvegicus | |
| 3,3-Dimethylglutamic acid | not at pH 7.0, weak at pH 8.0 | Rattus norvegicus | |
| 3-oxoadipate | inhibition at pH 7.0 is higher than at pH 8.5, 3.33 mM in presence of equimolar concentrations of oxaloacetate | Rattus norvegicus | |
| adipic acid | not at pH 7.0, weak at pH 8.0 | Rattus norvegicus | |
| Benzylmalonic acid | not at pH 7.0, weak at pH 8.0 | Rattus norvegicus | |
| Diethylstilbestrol diphosphate | - |
Rattus norvegicus | |
| L-aspartate | - |
Rattus norvegicus | |
| L-glutamate | - |
Rattus norvegicus | |
| pyridoxal 5'-phosphate | - |
Rattus norvegicus | |
| pyridoxamine phosphate | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
oxaloacetate | somewhat higher at very high levels of pyridoxamine, 14 mM | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyridoxamine + oxaloacetate | Rattus norvegicus | - |
pyridoxal + L-aspartate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 979fold | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.066 | - |
purified enzyme | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| frozen, 20% loss of activity after 2 months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyridoxamine + oxaloacetate | - |
Rattus norvegicus | pyridoxal + L-aspartate | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | about 50% of activity maximum at pH 7.5 and pH 9.5 | Rattus norvegicus |
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Release 2023.1 (January 2023)
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