BRENDA - Enzyme Database show
show all sequences of 2.6.1.31

Enzymatic transamination of pyridoxamine. I. With oxaloacetate and alpha-ketoglutarate

Wada, H.; Snell, E.E.; J. Biol. Chem. 237, 127-132 (1962)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
alpha-glycerophosphate
-
Escherichia coli
alpha-glycerophosphate
-
Oryctolagus cuniculus
beta-Glycerophosphate
-
Escherichia coli
beta-Glycerophosphate
-
Oryctolagus cuniculus
L-aspartate
no inhibition by D-aspartate
Oryctolagus cuniculus
L-glutamate
no inhibition by D-glutamate
Oryctolagus cuniculus
pyridoxal 5'-phosphate
-
Escherichia coli
pyridoxal 5'-phosphate
-
Oryctolagus cuniculus
pyridoxamine phosphate
-
Escherichia coli
pyridoxamine phosphate
-
Oryctolagus cuniculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.034
-
oxaloacetate
pH 8.5, 37C
Escherichia coli
0.57
-
oxaloacetate
pH 8.5, 37C
Oryctolagus cuniculus
0.7
-
pyridoxamine
pH 8.5, 37C
Oryctolagus cuniculus
2.3
-
pyridoxamine
pH 8.5, 37C
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pyridoxamine + oxaloacetate
Escherichia coli
-
pyridoxal + L-aspartate
-
Escherichia coli
r
pyridoxamine + oxaloacetate
Oryctolagus cuniculus
-
pyridoxal + L-aspartate
-
Oryctolagus cuniculus
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
-
Oryctolagus cuniculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Escherichia coli
additional information
-
-
Oryctolagus cuniculus
0.076
-
partially purified enzyme
Escherichia coli
0.081
-
partially purified enzyme
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no substrates are: pyruvate, 2-oxoglutarate, 3-oxoglutarate, 2-oxisovalerate, 2-oxoisocaproate, 2-oxocaproate, phenylpyruvate, mesoxalate, ketopantoate
639961
Escherichia coli
?
-
-
-
-
additional information
no substrates are: pyruvate, 2-oxoglutarate, 3-oxoglutarate, 2-oxisovalerate, 2-oxoisocaproate, 2-oxocaproate, phenylpyruvate, mesoxalate, ketopantoate
639961
Oryctolagus cuniculus
?
-
-
-
-
pyridoxamine + oxaloacetate
-
639961
Escherichia coli
pyridoxal + L-aspartate
-
639961
Escherichia coli
r
pyridoxamine + oxaloacetate
-
639961
Oryctolagus cuniculus
pyridoxal + L-aspartate
-
639961
Oryctolagus cuniculus
r
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Escherichia coli
37
-
assay at
Oryctolagus cuniculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Escherichia coli
8.5
-
-
Oryctolagus cuniculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
alpha-glycerophosphate
-
Escherichia coli
alpha-glycerophosphate
-
Oryctolagus cuniculus
beta-Glycerophosphate
-
Escherichia coli
beta-Glycerophosphate
-
Oryctolagus cuniculus
L-aspartate
no inhibition by D-aspartate
Oryctolagus cuniculus
L-glutamate
no inhibition by D-glutamate
Oryctolagus cuniculus
pyridoxal 5'-phosphate
-
Escherichia coli
pyridoxal 5'-phosphate
-
Oryctolagus cuniculus
pyridoxamine phosphate
-
Escherichia coli
pyridoxamine phosphate
-
Oryctolagus cuniculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.034
-
oxaloacetate
pH 8.5, 37C
Escherichia coli
0.57
-
oxaloacetate
pH 8.5, 37C
Oryctolagus cuniculus
0.7
-
pyridoxamine
pH 8.5, 37C
Oryctolagus cuniculus
2.3
-
pyridoxamine
pH 8.5, 37C
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pyridoxamine + oxaloacetate
Escherichia coli
-
pyridoxal + L-aspartate
-
Escherichia coli
r
pyridoxamine + oxaloacetate
Oryctolagus cuniculus
-
pyridoxal + L-aspartate
-
Oryctolagus cuniculus
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
-
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Escherichia coli
additional information
-
-
Oryctolagus cuniculus
0.076
-
partially purified enzyme
Escherichia coli
0.081
-
partially purified enzyme
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no substrates are: pyruvate, 2-oxoglutarate, 3-oxoglutarate, 2-oxisovalerate, 2-oxoisocaproate, 2-oxocaproate, phenylpyruvate, mesoxalate, ketopantoate
639961
Escherichia coli
?
-
-
-
-
additional information
no substrates are: pyruvate, 2-oxoglutarate, 3-oxoglutarate, 2-oxisovalerate, 2-oxoisocaproate, 2-oxocaproate, phenylpyruvate, mesoxalate, ketopantoate
639961
Oryctolagus cuniculus
?
-
-
-
-
pyridoxamine + oxaloacetate
-
639961
Escherichia coli
pyridoxal + L-aspartate
-
639961
Escherichia coli
r
pyridoxamine + oxaloacetate
-
639961
Oryctolagus cuniculus
pyridoxal + L-aspartate
-
639961
Oryctolagus cuniculus
r
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Escherichia coli
37
-
assay at
Oryctolagus cuniculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Escherichia coli
8.5
-
-
Oryctolagus cuniculus
Other publictions for EC 2.6.1.31
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
639962
Wu
Pyridoxamine-oxaloacetic trans ...
Rattus norvegicus
J. Biol. Chem.
239
1492-1497
1964
4
-
-
-
-
-
11
1
-
-
-
1
-
1
-
-
1
-
-
1
1
1
1
-
-
-
-
-
1
1
-
-
-
-
-
4
-
-
-
-
-
-
-
11
-
1
-
-
-
1
-
-
-
1
-
1
1
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
639961
Wada
Enzymatic transamination of py ...
Escherichia coli, Oryctolagus cuniculus
J. Biol. Chem.
237
127-132
1962
-
-
-
-
-
-
10
4
-
-
-
2
-
2
-
-
2
-
-
1
4
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
4
-
-
-
2
-
-
-
2
-
1
4
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-