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Literature summary for 2.6.1.30 extracted from

  • Yoshikane, Y.; Tamura, A.; Yokochi, N.; Ellouze, K.; Yamamura, E.; Mizunaga, H.; Fujimoto, N.; Sakamoto, K.; Sawa, Y.; Yagi, T.
    Engineering Mesorhizobium loti pyridoxamine-pyruvate aminotransferase for production of pyridoxamine with L-glutamate as an amino donor (2010), J. Mol. Catal. B, 67, 104-110.
No PubMed abstract available
Search for more literature for 2.6.1.30

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Mesorhizobium loti

Protein Variants

Protein Variants Comment Organism
M2T/V70K M2: surface residue Mesorhizobium loti
M2T/V70K/E212G E212: surface residue Mesorhizobium loti
M2T/Y35H/V70K Y35: active site residur Mesorhizobium loti
M2T/Y35H/V70K/E212G E212: surface residue Mesorhizobium loti
V70K active site residue, increase in reactivity toward 2-oxoglutarate Mesorhizobium loti
V70K/E212G E212: surface residue Mesorhizobium loti
V70R active site residue, elimination of activity Mesorhizobium loti
V70R/F247C F247: hydrophobic core residue Mesorhizobium loti
Y35H/V70K active site residues Mesorhizobium loti
Y35H/V70R/F247C F247: hydrophobic core residue Mesorhizobium loti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.8
-
 2-oxoglutarate Y35H/V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
12.6
-
 2-oxoglutarate Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
17.2
-
 2-oxoglutarate M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
21.1
-
 2-oxoglutarate M2T/Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
29.3
-
 L-glutamate Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
42.4
-
 L-glutamate Y35H/V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
44.2
-
 L-glutamate M2T/Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
51
-
 L-glutamate M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
61.3
-
 2-oxoglutarate V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
77.3
-
 2-oxoglutarate M2T/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
80.1
-
 2-oxoglutarate wild type protein, pH 9.0, 30°C Mesorhizobium loti
102.6
-
 2-oxoglutarate V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
106.8
-
 2-oxoglutarate M2T/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
110.3
-
 2-oxoglutarate V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
123.4
-
 L-glutamate wild type protein, pH 9.0, 30°C Mesorhizobium loti
133.5
-
 L-glutamate V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
142
-
 L-glutamate V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 4 * 41000, SDS-PAGE, gel filtration Mesorhizobium loti
154000
-
 gel filtration Mesorhizobium loti

Organism

Organism UniProt Comment Textmining
Mesorhizobium loti Q988B8
-
-

Purification (Commentary)

Purification (Comment) Organism
hydrophobic interaction chromatography, gel filtration Mesorhizobium loti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxamine + 2-oxoglutarate
-
 Mesorhizobium loti pyridoxal + L-glutamate
-
 r
pyridoxamine + pyruvate
-
 Mesorhizobium loti pyridoxal + L-alanine
-
 r

Subunits

Subunits Comment Organism
homotetramer 4 * 41000, SDS-PAGE, gel filtration Mesorhizobium loti

Synonyms

Synonyms Comment Organism
pyridoxamine-pyruvate aminotransferase pyridoxamine-2-oxoglutarate aminotransferase activity Mesorhizobium loti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
 L-glutamate wild type protein, pH 9.0, 30°C Mesorhizobium loti
1.9
-
 2-oxoglutarate wild type protein, pH 9.0, 30°C Mesorhizobium loti
2.7
-
 L-glutamate V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
2.9
-
 L-glutamate Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
3
-
 L-glutamate M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
3.1
-
 L-glutamate M2T/Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
3.5
-
 2-oxoglutarate M2T/Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
3.9
-
 2-oxoglutarate M2T/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
4
-
 2-oxoglutarate M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
4.1
-
 2-oxoglutarate V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
4.4
-
 L-glutamate V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
4.6
-
 2-oxoglutarate M2T/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
4.6
-
 2-oxoglutarate V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
5.2
-
 2-oxoglutarate Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
6.4
-
 L-glutamate Y35H/V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
8.1
-
 2-oxoglutarate Y35H/V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
9.1
-
 2-oxoglutarate V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.012
-
 L-glutamate wild type protein, pH 9.0, 30°C Mesorhizobium loti
0.019
-
 L-glutamate V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
0.023
-
 2-oxoglutarate wild type protein, pH 9.0, 30°C Mesorhizobium loti
0.033
-
 L-glutamate V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
0.04
-
 2-oxoglutarate V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
0.042
-
 2-oxoglutarate V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
0.043
-
 2-oxoglutarate M2T/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
0.05
-
 2-oxoglutarate M2T/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
0.058
-
 L-glutamate M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
0.07
-
 L-glutamate M2T/Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
0.1
-
 L-glutamate Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
0.148
-
 2-oxoglutarate V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
0.152
-
 L-glutamate Y35H/V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti
0.167
-
 2-oxoglutarate M2T/Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
0.234
-
 2-oxoglutarate M2T/Y35H/V70K/E212G mutant protein, pH 9.0, 30°C Mesorhizobium loti
0.408
-
 2-oxoglutarate Y35H/V70K mutant protein, pH 9.0, 30°C Mesorhizobium loti
0.916
-
 2-oxoglutarate Y35H/V70R/F247C mutant protein, pH 9.0, 30°C Mesorhizobium loti