Pyridoxamine-pyruvate transaminase. 2. Temperature-jump and stopped-flow kinetic investigation of the rates and mechanism of the reaction of 5-deoxypyridoxal with the enzyme

Gilmer, P.J.; Kirsch, J.F.; Biochemistry 16, 5246-5253 (1977)

Data extracted from this reference:

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

pyridoxamine + pyruvate

Pseudomonas sp.

-

pyridoxal + L-alanine

-

Pseudomonas sp.

r

Reaction

Commentary

Organism

Reaction ID

pyridoxamine + pyruvate = pyridoxal + L-alanine

mechanism

Pseudomonas sp.

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

additional information

temperature-jump and stopped-flow kinetic investigation of the rate and mechanism of the reaction of 5'-deoxy-pyridoxal with the enzyme

639960

Pseudomonas sp.

?

-

-

-

-

pyridoxamine + pyruvate

-

639960

Pseudomonas sp.

pyridoxal + L-alanine

-

639960

Pseudomonas sp.

r

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

pyridoxamine + pyruvate

Pseudomonas sp.

-

pyridoxal + L-alanine

-

Pseudomonas sp.

r

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

additional information

temperature-jump and stopped-flow kinetic investigation of the rate and mechanism of the reaction of 5'-deoxy-pyridoxal with the enzyme

639960

Pseudomonas sp.

?

-

-

-

-

pyridoxamine + pyruvate

-

639960

Pseudomonas sp.

pyridoxal + L-alanine

-

639960

Pseudomonas sp.

r

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

739366

Huang

-

Enzymatic transamination of py ...

Nicotiana tabacum

Plant Sci.

212

55-59

2013

-

-

-

-

-

-

1

2

-

-

1

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1

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1

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1

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3

1

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1

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1

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1

-

2

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1

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1

-

1

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-

3

1

1

-

-

-

1

-

-

-

-

-

-

-

-

-

722999

Yoshikane

-

Engineering Mesorhizobium loti ...

Mesorhizobium loti

J. Mol. Catal. B

67

104-110

2010

-

-

1

-

10

-

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17

-

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2

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1

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1

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2

1

1

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16

-

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1

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10

-

-

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17

-

-

2

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1

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-

2

1

-

-

-

16

-

-

-

-

-

-

-

-

17

17

687694

Yoshikane

Crystal structure of pyridoxam ...

Mesorhizobium loti, Mesorhizobium loti MAFF303099

J. Biol. Chem.

283

1120-1127

2008

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-

1

1

3

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1

1

3

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7

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4

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5

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-

688568

Nishimura

Determination of individual vi ...

Mesorhizobium loti

J. Nutr. Sci. Vitaminol.

54

18-24

2008

-

1

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1

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671864

Yoshikane

Molecular cloning, expression ...

Mesorhizobium loti, Mesorhizobium loti MAFF303099

Biochem. J.

396

499-507

2006

-

-

1

-

2

-

1

6

-

-

3

-

-

15

-

-

1

-

-

-

-

-

10

2

4

1

1

1

6

1

-

-

1

-

-

-

-

-

2

1

-

4

-

-

1

-

12

-

-

6

-

-

-

-

2

-

-

-

-

10

2

2

1

1

12

1

-

-

-

-

-

-

-

-

-

670891

Yanee

-

Vitamin B6 degradation by pyri ...

Ochrobactrum anthropi

Science Asia

31

307-311

2005

-

-

-

-

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1

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1

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1

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1

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-

1

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1

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1

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639958

Ford

-

Effect of pH and temperature o ...

Soil bacterium

J. Ala. Acad. Sci.

49

16-30

1978

-

-

-

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1

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1

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1

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1

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1

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1

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2

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1

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-

-

-

-

639959

Gilmer

Pyridoxamine-pyruvate transami ...

Pseudomonas sp.

Biochemistry

16

5241-5246

1977

-

-

-

-

-

1

-

1

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1

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2

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1

-

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1

3

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-

1

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1

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1

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1

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2

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-

1

3

-

1

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-

-

-

-

-

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-

-

-

-

639960

Gilmer

Pyridoxamine-pyruvate transami ...

Pseudomonas sp.

Biochemistry

16

5246-5253

1977

-

-

-

-

-

-

-

1

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1

-

1

-

-

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1

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2

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-

1

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-

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1

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1

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2

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1

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-

-

-

639957

Ayling

Relation of structure to activ ...

Pseudomonas sp.

Biochemistry

7

1626-1636

1968

-

-

-

-

-

-

5

20

-

-

-

1

-

1

-

-

-

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6

-

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5

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20

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1

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-

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6

-

-

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-

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-

-

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-

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-

-

-

639956

Wada

Enzymatic transamination pf py ...

Pseudomonas sp.

J. Biol. Chem.

237

133-137

1962

1

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1

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1

2

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1

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1

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1

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1

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1

1

1

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1

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1

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