BRENDA - Enzyme Database show
show all sequences of 2.6.1.30

Pyridoxamine-pyruvate transaminase. 1. Determination of the active site stoichiometry and the pH dependence of the dissociation constant for 5-deoxypyridoxal

Gilmer, P.J.; McIntire, W.S.; Kirsch, J.F.; Biochemistry 16, 5241-5246 (1977)

Data extracted from this reference:

General Stability
General Stability
Organism
K+ stabilizes
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Pseudomonas sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
activates
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Pseudomonas sp.
enzyme is part of the degradative pathway for vitamin B6 compounds utilized by Pseudomonas sp. MA-1
?
-
-
-
pyridoxamine + pyruvate
Pseudomonas sp.
-
pyridoxal + L-alanine
-
Pseudomonas sp.
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Storage Stability
Storage Stability
Organism
loss of activity within 12 h at 25°C in sodium phosphate buffer, but is stable in potassium buffer under the same conditions
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
determination of the active site stoichiometry and the pH dependence of the dissociation constant for 5'-deoxypyridoxal
639959
Pseudomonas sp.
?
-
-
-
-
additional information
enzyme is part of the degradative pathway for vitamin B6 compounds utilized by Pseudomonas sp. MA-1
639959
Pseudomonas sp.
?
-
-
-
-
pyridoxamine + pyruvate
-
639959
Pseudomonas sp.
pyridoxal + L-alanine
-
639959
Pseudomonas sp.
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Pseudomonas sp.
General Stability (protein specific)
General Stability
Organism
K+ stabilizes
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Pseudomonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
activates
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Pseudomonas sp.
enzyme is part of the degradative pathway for vitamin B6 compounds utilized by Pseudomonas sp. MA-1
?
-
-
-
pyridoxamine + pyruvate
Pseudomonas sp.
-
pyridoxal + L-alanine
-
Pseudomonas sp.
r
Storage Stability (protein specific)
Storage Stability
Organism
loss of activity within 12 h at 25°C in sodium phosphate buffer, but is stable in potassium buffer under the same conditions
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
determination of the active site stoichiometry and the pH dependence of the dissociation constant for 5'-deoxypyridoxal
639959
Pseudomonas sp.
?
-
-
-
-
additional information
enzyme is part of the degradative pathway for vitamin B6 compounds utilized by Pseudomonas sp. MA-1
639959
Pseudomonas sp.
?
-
-
-
-
pyridoxamine + pyruvate
-
639959
Pseudomonas sp.
pyridoxal + L-alanine
-
639959
Pseudomonas sp.
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Pseudomonas sp.
Other publictions for EC 2.6.1.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739366
Huang
Enzymatic transamination of py ...
Nicotiana tabacum
Plant Sci.
212
55-59
2013
-
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1
2
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1
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1
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1
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1
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3
1
1
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1
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1
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1
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1
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1
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3
1
1
-
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1
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-
722999
Yoshikane
-
Engineering Mesorhizobium loti ...
Mesorhizobium loti
J. Mol. Catal. B
67
104-110
2010
-
-
1
-
10
-
-
17
-
-
2
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-
1
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1
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-
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-
2
1
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-
16
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1
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10
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17
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2
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1
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2
1
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16
-
-
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17
17
687694
Yoshikane
Crystal structure of pyridoxam ...
Mesorhizobium loti, Mesorhizobium loti MAFF303099
J. Biol. Chem.
283
1120-1127
2008
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-
1
1
3
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7
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4
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1
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4
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5
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1
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1
1
1
3
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7
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4
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5
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688568
Nishimura
Determination of individual vi ...
Mesorhizobium loti
J. Nutr. Sci. Vitaminol.
54
18-24
2008
-
1
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1
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671864
Yoshikane
Molecular cloning, expression ...
Mesorhizobium loti, Mesorhizobium loti MAFF303099
Biochem. J.
396
499-507
2006
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1
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2
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1
6
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-
3
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14
-
-
1
-
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-
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-
10
2
1
1
1
6
1
-
-
1
-
-
-
-
-
2
1
-
4
-
-
1
-
12
-
-
6
-
-
-
-
2
-
-
-
-
10
2
2
1
1
12
1
-
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670891
Yanee
-
Vitamin B6 degradation by pyri ...
Ochrobactrum anthropi
Science Asia
31
307-311
2005
-
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1
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1
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1
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1
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1
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1
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639958
Ford
-
Effect of pH and temperature o ...
Soil bacterium
J. Ala. Acad. Sci.
49
16-30
1978
-
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1
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1
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1
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1
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1
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1
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2
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1
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-
639959
Gilmer
Pyridoxamine-pyruvate transami ...
Pseudomonas sp.
Biochemistry
16
5241-5246
1977
-
-
-
-
-
1
-
1
-
1
-
2
-
1
-
-
-
-
-
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-
1
3
-
1
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-
-
1
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1
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1
-
2
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-
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-
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1
3
-
1
-
-
-
-
-
-
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-
639960
Gilmer
Pyridoxamine-pyruvate transami ...
Pseudomonas sp.
Biochemistry
16
5246-5253
1977
-
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1
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1
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1
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1
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1
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2
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1
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-
639957
Ayling
Relation of structure to activ ...
Pseudomonas sp.
Biochemistry
7
1626-1636
1968
-
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5
20
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1
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1
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6
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5
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20
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1
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6
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639956
Wada
Enzymatic transamination pf py ...
Pseudomonas sp.
J. Biol. Chem.
237
133-137
1962
1
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1
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1
2
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1
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1
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1
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1
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1
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1
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1
1
1
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1
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1
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1
1
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1
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2
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1
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1
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1
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1
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1
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1
1
1
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