BRENDA - Enzyme Database show
show all sequences of 2.6.1.30

Relation of structure to activity of pyridoxal analogs as substrates for pyridoxamine pyruvate transaminase

Ayling, J.E.; Snell, E.E.; Biochemistry 7, 1626-1636 (1968)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
3-Deoxypyridoxal
inhibits pyridoxamine formation from pyridoxal + alanine
Pseudomonas sp.
4-Nitrosalicylaldehyde
inhibits pyridoxamine formation from pyridoxal + alanine
Pseudomonas sp.
N-Methylpyridoxal
inhibits pyridoxamine formation from pyridoxal + alanine
Pseudomonas sp.
O-Methylpyridoxal
inhibits pyridoxamine formation from pyridoxal + alanine
Pseudomonas sp.
Pyridine-4-aldehyde
inhibits pyridoxamine formation from pyridoxal + alanine
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Pseudomonas sp.
0.009
-
5-Deoxypyridoxal
pH 8.9, 25C
Pseudomonas sp.
0.012
-
pyridoxal
pH 8.9, 25C
Pseudomonas sp.
0.013
-
pyridoxamine
pH 8.9, 25C
Pseudomonas sp.
0.014
-
5-Deoxypyridoxamine
pH 8.9, 25C
Pseudomonas sp.
0.16
-
2-Norpyridoxamine
pH 8.9, 25C
Pseudomonas sp.
0.35
-
pyridoxamine
with 3-hydroxy-4-aminomethylpyridine, pH 8.9, 25C
Pseudomonas sp.
0.35
-
pyruvate
with 3-hydroxy-4-aminomethylpyridine, pH 8.9, 25C
Pseudomonas sp.
0.4
-
pyruvate
with omega-methylpyridoxamine, pH 8.9, 25C
Pseudomonas sp.
0.42
-
pyruvate
with 5-deoxypyridoxamine, pH 8.9, 25C
Pseudomonas sp.
0.52
-
pyruvate
with 2-norpyridoxamine, pH 8.9, 25C
Pseudomonas sp.
0.58
-
L-alanine
with omega-methylpyridoxal, pH 8.9, 25C
Pseudomonas sp.
0.59
-
2-norpyridoxal
pH 8.9, 25C
Pseudomonas sp.
0.89
-
3-hydroxypyridine-4-aldehyde
pH 8.9, 25C
Pseudomonas sp.
1.3
-
omega-methylpyridoxal
pH 8.9, 25C
Pseudomonas sp.
1.6
-
L-alanine
with pyridoxal, pH 8.9, 25C
Pseudomonas sp.
1.9
-
L-alanine
with 5-deoxypyridoxal, pH 8.9, 25C
Pseudomonas sp.
2.7
-
L-alanine
with 2-norpyridoxal, pH 8.9, 25C
Pseudomonas sp.
7.4
-
pyruvate
with 3-hydroxy-4-aminomethylpyridine, pH 8.9, 25C
Pseudomonas sp.
64.5
-
L-alanine
with 3-hydroxypyridine-4-aldehyde, pH 8.9, 25C
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pyridoxamine + pyruvate
Pseudomonas sp.
-
pyridoxal + L-alanine
-
Pseudomonas sp.
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-norpyridoxal + L-alanine
-
639957
Pseudomonas sp.
2-norpyridoxamine + pyruvate
-
639957
Pseudomonas sp.
r
3-hydroxypyridine-4-aldehyde + L-alanine
-
639957
Pseudomonas sp.
3-hydroxy-4-aminomethylpyridine + pyruvate
-
639957
Pseudomonas sp.
r
5-deoxypyridoxal + L-alanine
-
639957
Pseudomonas sp.
5-deoxypyridoxamine + pyruvate
-
639957
Pseudomonas sp.
r
additional information
not active with: 3-deoxypyridoxal, pyridine-4-aldehyde, O-methylpyridoxal, 4-nitrosalicylaldehyde
639957
Pseudomonas sp.
?
-
-
-
-
omega-methylpyridoxal + L-alanine
i.e. 3-hydroxy-5-hydroxymethyl-2-ethylpyridine-4-carboxaldehyde
639957
Pseudomonas sp.
omega-methylpyridoxamine + pyruvate
-
639957
Pseudomonas sp.
r
pyridoxamine + pyruvate
-
639957
Pseudomonas sp.
pyridoxal + L-alanine
-
639957
Pseudomonas sp.
r
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-Deoxypyridoxal
inhibits pyridoxamine formation from pyridoxal + alanine
Pseudomonas sp.
4-Nitrosalicylaldehyde
inhibits pyridoxamine formation from pyridoxal + alanine
Pseudomonas sp.
N-Methylpyridoxal
inhibits pyridoxamine formation from pyridoxal + alanine
Pseudomonas sp.
O-Methylpyridoxal
inhibits pyridoxamine formation from pyridoxal + alanine
Pseudomonas sp.
Pyridine-4-aldehyde
inhibits pyridoxamine formation from pyridoxal + alanine
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Pseudomonas sp.
0.009
-
5-Deoxypyridoxal
pH 8.9, 25C
Pseudomonas sp.
0.012
-
pyridoxal
pH 8.9, 25C
Pseudomonas sp.
0.013
-
pyridoxamine
pH 8.9, 25C
Pseudomonas sp.
0.014
-
5-Deoxypyridoxamine
pH 8.9, 25C
Pseudomonas sp.
0.16
-
2-Norpyridoxamine
pH 8.9, 25C
Pseudomonas sp.
0.35
-
pyridoxamine
with 3-hydroxy-4-aminomethylpyridine, pH 8.9, 25C
Pseudomonas sp.
0.35
-
pyruvate
with 3-hydroxy-4-aminomethylpyridine, pH 8.9, 25C
Pseudomonas sp.
0.4
-
pyruvate
with omega-methylpyridoxamine, pH 8.9, 25C
Pseudomonas sp.
0.42
-
pyruvate
with 5-deoxypyridoxamine, pH 8.9, 25C
Pseudomonas sp.
0.52
-
pyruvate
with 2-norpyridoxamine, pH 8.9, 25C
Pseudomonas sp.
0.58
-
L-alanine
with omega-methylpyridoxal, pH 8.9, 25C
Pseudomonas sp.
0.59
-
2-norpyridoxal
pH 8.9, 25C
Pseudomonas sp.
0.89
-
3-hydroxypyridine-4-aldehyde
pH 8.9, 25C
Pseudomonas sp.
1.3
-
omega-methylpyridoxal
pH 8.9, 25C
Pseudomonas sp.
1.6
-
L-alanine
with pyridoxal, pH 8.9, 25C
Pseudomonas sp.
1.9
-
L-alanine
with 5-deoxypyridoxal, pH 8.9, 25C
Pseudomonas sp.
2.7
-
L-alanine
with 2-norpyridoxal, pH 8.9, 25C
Pseudomonas sp.
7.4
-
pyruvate
with 3-hydroxy-4-aminomethylpyridine, pH 8.9, 25C
Pseudomonas sp.
64.5
-
L-alanine
with 3-hydroxypyridine-4-aldehyde, pH 8.9, 25C
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pyridoxamine + pyruvate
Pseudomonas sp.
-
pyridoxal + L-alanine
-
Pseudomonas sp.
r
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-norpyridoxal + L-alanine
-
639957
Pseudomonas sp.
2-norpyridoxamine + pyruvate
-
639957
Pseudomonas sp.
r
3-hydroxypyridine-4-aldehyde + L-alanine
-
639957
Pseudomonas sp.
3-hydroxy-4-aminomethylpyridine + pyruvate
-
639957
Pseudomonas sp.
r
5-deoxypyridoxal + L-alanine
-
639957
Pseudomonas sp.
5-deoxypyridoxamine + pyruvate
-
639957
Pseudomonas sp.
r
additional information
not active with: 3-deoxypyridoxal, pyridine-4-aldehyde, O-methylpyridoxal, 4-nitrosalicylaldehyde
639957
Pseudomonas sp.
?
-
-
-
-
omega-methylpyridoxal + L-alanine
i.e. 3-hydroxy-5-hydroxymethyl-2-ethylpyridine-4-carboxaldehyde
639957
Pseudomonas sp.
omega-methylpyridoxamine + pyruvate
-
639957
Pseudomonas sp.
r
pyridoxamine + pyruvate
-
639957
Pseudomonas sp.
pyridoxal + L-alanine
-
639957
Pseudomonas sp.
r
Other publictions for EC 2.6.1.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739366
Huang
Enzymatic transamination of py ...
Nicotiana tabacum
Plant Sci.
212
55-59
2013
-
-
-
-
-
-
1
2
-
-
1
-
-
1
-
-
1
-
-
1
-
-
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
1
-
1
-
-
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
722999
Yoshikane
-
Engineering Mesorhizobium loti ...
Mesorhizobium loti
J. Mol. Catal. B
67
104-110
2010
-
-
1
-
10
-
-
17
-
-
2
-
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
16
-
-
-
-
-
-
-
-
-
1
-
-
10
-
-
-
-
17
-
-
2
-
-
-
-
1
-
-
-
-
2
1
-
-
-
16
-
-
-
-
-
-
-
-
17
17
687694
Yoshikane
Crystal structure of pyridoxam ...
Mesorhizobium loti, Mesorhizobium loti MAFF303099
J. Biol. Chem.
283
1120-1127
2008
-
-
1
1
3
-
-
7
-
-
-
-
-
4
-
-
-
1
-
-
-
-
4
-
-
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-
5
-
-
-
1
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-
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1
1
1
3
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
688568
Nishimura
Determination of individual vi ...
Mesorhizobium loti
J. Nutr. Sci. Vitaminol.
54
18-24
2008
-
1
-
-
-
-
-
-
-
-
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-
-
1
-
-
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1
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-
-
671864
Yoshikane
Molecular cloning, expression ...
Mesorhizobium loti, Mesorhizobium loti MAFF303099
Biochem. J.
396
499-507
2006
-
-
1
-
2
-
1
6
-
-
3
-
-
14
-
-
1
-
-
-
-
-
10
2
1
1
1
6
1
-
-
1
-
-
-
-
-
2
1
-
4
-
-
1
-
12
-
-
6
-
-
-
-
2
-
-
-
-
10
2
2
1
1
12
1
-
-
-
-
-
-
-
-
-
670891
Yanee
-
Vitamin B6 degradation by pyri ...
Ochrobactrum anthropi
Science Asia
31
307-311
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639958
Ford
-
Effect of pH and temperature o ...
Soil bacterium
J. Ala. Acad. Sci.
49
16-30
1978
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
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-
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-
2
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1
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1
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1
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-
2
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-
1
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-
-
-
-
-
639959
Gilmer
Pyridoxamine-pyruvate transami ...
Pseudomonas sp.
Biochemistry
16
5241-5246
1977
-
-
-
-
-
1
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
1
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
2
-
-
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-
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-
1
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
639960
Gilmer
Pyridoxamine-pyruvate transami ...
Pseudomonas sp.
Biochemistry
16
5246-5253
1977
-
-
-
-
-
-
-
1
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-
1
-
1
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1
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2
-
1
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1
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1
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2
-
1
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-
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-
639957
Ayling
Relation of structure to activ ...
Pseudomonas sp.
Biochemistry
7
1626-1636
1968
-
-
-
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-
5
20
-
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-
1
-
1
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6
-
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5
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20
-
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1
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6
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639956
Wada
Enzymatic transamination pf py ...
Pseudomonas sp.
J. Biol. Chem.
237
133-137
1962
1
-
-
1
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1
2
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-
1
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1
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1
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1
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1
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1
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1
1
1
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1
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1
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1
1
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1
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2
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1
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1
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1
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1
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1
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1
1
1
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