Data extracted from this reference:
Activating Compound
additional information
phosphate is neither essential nor inhibitory
Pseudomonas sp.
Crystallization (Commentary)
addition of saturated ammonium sulfate solution to the concentrated protein solution until the first permanent turbidity appears, several days at 5°C
Pseudomonas sp.
Inhibitors
additional information
phosphate is neither essential nor inhibitory
Pseudomonas sp.
KM Value [mM]
0.043
pyridoxamine
pH 8.5, 37°C
Pseudomonas sp.
0.1
pyruvate
pH 8.5, 37°C
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
pyridoxamine + pyruvate
Pseudomonas sp.
oxaloacetate and several other keto acids tested cannot replace pyruvate as amino group acceptor
pyridoxal + L-alanine
Pseudomonas sp.
r
Organism
Purification (Commentary)
Specific Activity [micromol/min/mg]
7.3
purified enzyme
Pseudomonas sp.
Substrates and Products (Substrate)
pyridoxamine + pyruvate
oxaloacetate and several other keto acids tested cannot replace pyruvate as amino group acceptor
639956
Pseudomonas sp.
pyridoxal + L-alanine
639956
Pseudomonas sp.
r
Temperature Optimum [°C]
37
assay at
Pseudomonas sp.
Temperature Stability [°C]
70
10 min, stable
Pseudomonas sp.
Turnover Number [1/s]
additional information
additional information
Pseudomonas sp.
pH Optimum
Cofactor
additional information
pyridoxal 5'-phosphate is not required for activity
Pseudomonas sp.
Activating Compound (protein specific)
additional information
phosphate is neither essential nor inhibitory
Pseudomonas sp.
Cofactor (protein specific)
additional information
pyridoxal 5'-phosphate is not required for activity
Pseudomonas sp.
Crystallization (Commentary) (protein specific)
addition of saturated ammonium sulfate solution to the concentrated protein solution until the first permanent turbidity appears, several days at 5°C
Pseudomonas sp.
Inhibitors (protein specific)
additional information
phosphate is neither essential nor inhibitory
Pseudomonas sp.
KM Value [mM] (protein specific)
0.043
pyridoxamine
pH 8.5, 37°C
Pseudomonas sp.
0.1
pyruvate
pH 8.5, 37°C
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
pyridoxamine + pyruvate
Pseudomonas sp.
oxaloacetate and several other keto acids tested cannot replace pyruvate as amino group acceptor
pyridoxal + L-alanine
Pseudomonas sp.
r
Purification (Commentary) (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
7.3
purified enzyme
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
pyridoxamine + pyruvate
oxaloacetate and several other keto acids tested cannot replace pyruvate as amino group acceptor
639956
Pseudomonas sp.
pyridoxal + L-alanine
639956
Pseudomonas sp.
r
Temperature Optimum [°C] (protein specific)
37
assay at
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
70
10 min, stable
Pseudomonas sp.
Turnover Number [1/s] (protein specific)
additional information
additional information
Pseudomonas sp.
pH Optimum (protein specific)
Other publictions for EC
739366
Huang
-
Enzymatic transamination of py ...
Nicotiana tabacum
Plant Sci.
212
55-59
2013
-
-
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1
2
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-
1
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1
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1
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1
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3
1
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1
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1
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1
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2
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1
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1
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1
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3
1
1
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1
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-
-
-
-
722999
Yoshikane
-
Engineering Mesorhizobium loti ...
Mesorhizobium loti
J. Mol. Catal. B
67
104-110
2010
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-
1
-
10
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-
17
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-
2
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1
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1
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2
1
1
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16
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1
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-
10
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17
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2
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1
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2
1
-
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-
16
-
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-
17
17
687694
Yoshikane
Crystal structure of pyridoxam ...
Mesorhizobium loti, Mesorhizobium loti MAFF303099
J. Biol. Chem.
283
1120-1127
2008
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-
1
1
3
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-
7
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-
4
-
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-
1
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-
4
-
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5
-
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1
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1
1
1
3
-
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7
-
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-
-
-
4
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
688568
Nishimura
Determination of individual vi ...
Mesorhizobium loti
J. Nutr. Sci. Vitaminol.
54
18-24
2008
-
1
-
-
-
-
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1
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1
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671864
Yoshikane
Molecular cloning, expression ...
Mesorhizobium loti, Mesorhizobium loti MAFF303099
Biochem. J.
396
499-507
2006
-
-
1
-
2
-
1
6
-
-
3
-
-
15
-
-
1
-
-
-
-
-
10
2
4
1
1
1
6
1
-
-
1
-
-
-
-
-
2
1
-
4
-
-
1
-
12
-
-
6
-
-
-
-
2
-
-
-
-
10
2
2
1
1
12
1
-
-
-
-
-
-
-
-
-
670891
Yanee
-
Vitamin B6 degradation by pyri ...
Ochrobactrum anthropi
Science Asia
31
307-311
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639958
Ford
-
Effect of pH and temperature o ...
Soil bacterium
J. Ala. Acad. Sci.
49
16-30
1978
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
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-
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-
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-
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-
-
1
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-
1
-
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-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
639959
Gilmer
Pyridoxamine-pyruvate transami ...
Pseudomonas sp.
Biochemistry
16
5241-5246
1977
-
-
-
-
-
1
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
1
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
1
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
639960
Gilmer
Pyridoxamine-pyruvate transami ...
Pseudomonas sp.
Biochemistry
16
5246-5253
1977
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
1
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-
-
1
-
-
-
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-
-
-
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2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
639957
Ayling
Relation of structure to activ ...
Pseudomonas sp.
Biochemistry
7
1626-1636
1968
-
-
-
-
-
-
5
20
-
-
-
1
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
20
-
-
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639956
Wada
Enzymatic transamination pf py ...
Pseudomonas sp.
J. Biol. Chem.
237
133-137
1962
1
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-
1
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1
2
-
-
-
1
-
1
-
-
1
-
-
-
1
-
1
-
-
1
-
1
1
1
-
-
1
-
-
-
1
-
-
1
1
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-
-
1
-
2
-
-
-
1
-
-
-
1
-
-
1
-
1
-
1
-
1
1
1
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-
-
-
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-
-
-
-