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Literature summary for 2.6.1.27 extracted from

  • Preuss, J.; Hort, W.; Lang, S.; Netsch, A.; Rahlfs, S.; Lochnit, G.; Jortzik, E.; Becker, K.; Mayser, P.A.
    Characterization of tryptophan aminotransferase 1 of Malassezia furfur, the key enzyme in the production of indolic compounds by M.furfur (2013), Exp. Dermatol., 22, 736-741.
    View publication on PubMed
Search for more literature for 2.6.1.27

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Malassezia furfur

Inhibitors

Inhibitors Comment Organism Structure
Aminooxyacetate
-
 Malassezia furfur
cycloserine
-
 Malassezia furfur

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.9
-
 2-oxoglutarate pH 8.0, 42°C Malassezia furfur
7
-
 L-tryptophan pH 8.0, 42°C Malassezia furfur

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
-
 Malassezia furfur
52500
-
-
 Malassezia furfur

Organism

Organism UniProt Comment Textmining
Malassezia furfur S4UF58
-
-
Malassezia furfur CBS 7019 S4UF58
-
-

Storage Stability

Storage Stability Organism
4°C, H2O, stable for around 1 week Malassezia furfur

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + 2-oxoglutarate
-
 Malassezia furfur (indol-3-yl)pyruvate + L-glutamate
-
 ?
L-tryptophan + 2-oxoglutarate
-
 Malassezia furfur CBS 7019 (indol-3-yl)pyruvate + L-glutamate
-
 ?
L-tryptophan + 2-oxomethylthiobutanoate 113% of the activity of 2-oxoglutarate as amine acceptor Malassezia furfur (indol-3-yl)pyruvate + 2-aminomethylthiobutanoate
-
 ?
L-tryptophan + 2-oxomethylthiobutanoate 113% of the activity of 2-oxoglutarate as amine acceptor Malassezia furfur CBS 7019 (indol-3-yl)pyruvate + 2-aminomethylthiobutanoate
-
 ?
L-tryptophan + phenylpyruvate 93% of the activity of 2-oxoglutarate as amine acceptor Malassezia furfur (indol-3-yl)pyruvate + L-phenylalanine
-
 ?
L-tryptophan + phenylpyruvate 93% of the activity of 2-oxoglutarate as amine acceptor Malassezia furfur CBS 7019 (indol-3-yl)pyruvate + L-phenylalanine
-
 ?
additional information no substrates: amine acceptors glyoxylic acid and hydroxypyruvate, amine donors L-phenylalanine, L-alanine, L-glycine, L-serine, L-histidine Malassezia furfur ?
-
 ?
additional information no substrates: amine acceptors glyoxylic acid and hydroxypyruvate, amine donors L-phenylalanine, L-alanine, L-glycine, L-serine, L-histidine Malassezia furfur CBS 7019 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE, x * 52500, calculated Malassezia furfur

Synonyms

Synonyms Comment Organism
Tam1
-
 Malassezia furfur

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Malassezia furfur

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Malassezia furfur

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate activity is highly dependent on the cofactor and increases in presence of additional cofactor Malassezia furfur

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.8
-
 pH 8.0, 42°C Malassezia furfur Aminooxyacetate
86
-
 pH 8.0, 42°C Malassezia furfur cycloserine