BRENDA - Enzyme Database
show all sequences of 2.6.1.26

L-Triiodothyronine aminotransferase

Soffer, R.L.; Hechtman, P.; Savage, M.; J. Biol. Chem. 248, 1224-1230 (1973)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
absolutely required
Oryctolagus cuniculus
Inhibitors
Inhibitors
Commentary
Organism
Structure
p-hydroxymercuribenzoate
complete inhibition at 0.1 mM
Oryctolagus cuniculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.034
-
L-triiodothyronine
pH 7.8, 37°C
Oryctolagus cuniculus
0.2
-
2-oxoglutarate
cosubstrate L-thyroxine, pH 7.8, 37°C; cosubstrate triiodothyronine, pH 7.8, 37°C
Oryctolagus cuniculus
0.9
-
3,5-diiodo-L-tyrosine
pH 7.8, 37°C
Oryctolagus cuniculus
2
-
2-oxoglutarate
cosubstrate 3,5-dinitro-L-tyrosine, pH 7.8, 37°C
Oryctolagus cuniculus
2
-
pyruvate
cosubstrate L-triiodothyronine, pH 7.8, 37°C
Oryctolagus cuniculus
2.12
-
L-thyronine
pH 7.8, 37°C
Oryctolagus cuniculus
3.8
-
3-iodo-L-tyrosine
pH 7.8, 37°C
Oryctolagus cuniculus
4.4
-
3,5-Dinitro-L-tyrosine
pH 7.8, 37°C
Oryctolagus cuniculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Oryctolagus cuniculus
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
2 * 48000, SDS-PAGE
Oryctolagus cuniculus
95000
-
glycerol density gradient centrifugation
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-3,5,3'-triiodothyronine + 2-oxoglutarate
Oryctolagus cuniculus
-
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
i.e. 3,5,3'-triiodothyropyruvate + L-Glu
Oryctolagus cuniculus
r
Organism
Organism
UniProt
Commentary
Textmining
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Oryctolagus cuniculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
low activity
Oryctolagus cuniculus
-
heart
low activity
Oryctolagus cuniculus
-
kidney
-
Oryctolagus cuniculus
-
liver
-
Oryctolagus cuniculus
-
lung
low activity
Oryctolagus cuniculus
-
additional information
not in serum
Oryctolagus cuniculus
-
skeletal muscle
low activity
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.013
-
purified enzyme, substrates L-3,5,3'-triiodothyronine + 2-oxoglutarate
Oryctolagus cuniculus
15
-
purified enzyme, substrates 3,5-dinitro-L-tyrosine + 2-oxoglutarate
Oryctolagus cuniculus
Storage Stability
Storage Stability
Organism
-20°C, 30% loss of activity per month
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3,5-dibromo-L-tyrosine + 2-oxoglutarate
-
639937
Oryctolagus cuniculus
3-(3,5-dibromo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
r
3,5-diiodo-D-tyrosine + 2-oxoglutarate
very low activity
639937
Oryctolagus cuniculus
3-(3,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
r
3,5-diiodo-L-tyrosine + 2-oxoglutarate
-
639937
Oryctolagus cuniculus
3-(3,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
r
3,5-dinitro-L-tyrosine + 2-oxoglutarate
-
639937
Oryctolagus cuniculus
3-(3,5-dinitro-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
i.e. 3,5-dinitro-p-hydroxyphenylpyruvic acid + Glu
639937
Oryctolagus cuniculus
r
3-amino-L-tyrosine + 2-oxoglutarate
very low activity
639937
Oryctolagus cuniculus
3-(3-amino-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
r
3-iodo-L-tyrosine + 2-oxoglutarate
-
639937
Oryctolagus cuniculus
3-(3-iodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
r
3-nitro-L-tyrosine + 2-oxoglutarate
-
639937
Oryctolagus cuniculus
3-(3-nitro-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
r
L-3,5,3'-triiodothyronine + 2-oxoglutarate
-
639937
Oryctolagus cuniculus
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
i.e. 3,5,3'-triiodothyropyruvate + L-Glu
639937
Oryctolagus cuniculus
r
L-3,5,3'-triiodothyronine + oxaloacetate
-
639937
Oryctolagus cuniculus
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + glycine
i.e. 3,5,3'-triiodothyrophenylpyruvate + Gly
639937
Oryctolagus cuniculus
r
L-3,5,3'-triiodothyronine + pyruvate
-
639937
Oryctolagus cuniculus
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-alanine
i.e. 3,5,3'-triiodothyrophenylpyruvate + L-Ala
639937
Oryctolagus cuniculus
r
L-phenylalanine + 2-oxoglutarate
low activtiy
639937
Oryctolagus cuniculus
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
r
L-thyroxine + 2-oxoglutarate
-
639937
Oryctolagus cuniculus
3-[4-(4-hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
-
-
-
r
L-tryptophan + 2-oxoglutarate
low activtiy
639937
Oryctolagus cuniculus
3-indole-2-oxopropanoate + L-glutamate
-
-
-
r
L-tyrosine + 2-oxoglutarate
low activtiy
639937
Oryctolagus cuniculus
2-oxo-3-phenylpropanoate + L-glutamate
-
-
-
r
additional information
specific for 2-oxoglutarate
639937
Oryctolagus cuniculus
?
-
-
-
-
additional information
pyruvate and oxalacetate are ineffective with 3,5-dinitro-L-tyrosine and only partially effective with iodinated tyrosines
639937
Oryctolagus cuniculus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 48000, SDS-PAGE
Oryctolagus cuniculus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0233
-
L-thyroxine
pH 7.8, 37°C
Oryctolagus cuniculus
0.0317
-
L-triiodothyronine
pH 7.8, 37°C
Oryctolagus cuniculus
1.1
-
3,5-diiodo-L-tyrosine
pH 7.8, 37°C
Oryctolagus cuniculus
3.68
-
3-iodo-L-tyrosine
pH 7.8, 37°C
Oryctolagus cuniculus
6.6
-
3,5-diiodo-L-tyrosine
pH 7.8, 37°C
Oryctolagus cuniculus
39.9
-
3,5-Dinitro-L-tyrosine
pH 7.8, 37°C
Oryctolagus cuniculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
8.6
broad optimum
Oryctolagus cuniculus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
8.6
pH 7.0: 30% of maximal activity with L-triiodothyronine, 15% of maximal activity with 3,5-dinitro-L-tyrosine, pH 7.4-8.6: maximal activity with triiodothyronine and 3,5-dinitro-L-tyrosine
Oryctolagus cuniculus
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
partial requirement
Oryctolagus cuniculus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
absolutely required
Oryctolagus cuniculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
partial requirement
Oryctolagus cuniculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
p-hydroxymercuribenzoate
complete inhibition at 0.1 mM
Oryctolagus cuniculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.034
-
L-triiodothyronine
pH 7.8, 37°C
Oryctolagus cuniculus
0.2
-
2-oxoglutarate
cosubstrate L-thyroxine, pH 7.8, 37°C; cosubstrate triiodothyronine, pH 7.8, 37°C
Oryctolagus cuniculus
0.9
-
3,5-diiodo-L-tyrosine
pH 7.8, 37°C
Oryctolagus cuniculus
2
-
2-oxoglutarate
cosubstrate 3,5-dinitro-L-tyrosine, pH 7.8, 37°C
Oryctolagus cuniculus
2
-
pyruvate
cosubstrate L-triiodothyronine, pH 7.8, 37°C
Oryctolagus cuniculus
2.12
-
L-thyronine
pH 7.8, 37°C
Oryctolagus cuniculus
3.8
-
3-iodo-L-tyrosine
pH 7.8, 37°C
Oryctolagus cuniculus
4.4
-
3,5-Dinitro-L-tyrosine
pH 7.8, 37°C
Oryctolagus cuniculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Oryctolagus cuniculus
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
2 * 48000, SDS-PAGE
Oryctolagus cuniculus
95000
-
glycerol density gradient centrifugation
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-3,5,3'-triiodothyronine + 2-oxoglutarate
Oryctolagus cuniculus
-
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
i.e. 3,5,3'-triiodothyropyruvate + L-Glu
Oryctolagus cuniculus
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
low activity
Oryctolagus cuniculus
-
heart
low activity
Oryctolagus cuniculus
-
kidney
-
Oryctolagus cuniculus
-
liver
-
Oryctolagus cuniculus
-
lung
low activity
Oryctolagus cuniculus
-
additional information
not in serum
Oryctolagus cuniculus
-
skeletal muscle
low activity
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.013
-
purified enzyme, substrates L-3,5,3'-triiodothyronine + 2-oxoglutarate
Oryctolagus cuniculus
15
-
purified enzyme, substrates 3,5-dinitro-L-tyrosine + 2-oxoglutarate
Oryctolagus cuniculus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 30% loss of activity per month
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3,5-dibromo-L-tyrosine + 2-oxoglutarate
-
639937
Oryctolagus cuniculus
3-(3,5-dibromo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
r
3,5-diiodo-D-tyrosine + 2-oxoglutarate
very low activity
639937
Oryctolagus cuniculus
3-(3,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
r
3,5-diiodo-L-tyrosine + 2-oxoglutarate
-
639937
Oryctolagus cuniculus
3-(3,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
r
3,5-dinitro-L-tyrosine + 2-oxoglutarate
-
639937
Oryctolagus cuniculus
3-(3,5-dinitro-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
i.e. 3,5-dinitro-p-hydroxyphenylpyruvic acid + Glu
639937
Oryctolagus cuniculus
r
3-amino-L-tyrosine + 2-oxoglutarate
very low activity
639937
Oryctolagus cuniculus
3-(3-amino-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
r
3-iodo-L-tyrosine + 2-oxoglutarate
-
639937
Oryctolagus cuniculus
3-(3-iodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
r
3-nitro-L-tyrosine + 2-oxoglutarate
-
639937
Oryctolagus cuniculus
3-(3-nitro-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
r
L-3,5,3'-triiodothyronine + 2-oxoglutarate
-
639937
Oryctolagus cuniculus
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
i.e. 3,5,3'-triiodothyropyruvate + L-Glu
639937
Oryctolagus cuniculus
r
L-3,5,3'-triiodothyronine + oxaloacetate
-
639937
Oryctolagus cuniculus
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + glycine
i.e. 3,5,3'-triiodothyrophenylpyruvate + Gly
639937
Oryctolagus cuniculus
r
L-3,5,3'-triiodothyronine + pyruvate
-
639937
Oryctolagus cuniculus
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-alanine
i.e. 3,5,3'-triiodothyrophenylpyruvate + L-Ala
639937
Oryctolagus cuniculus
r
L-phenylalanine + 2-oxoglutarate
low activtiy
639937
Oryctolagus cuniculus
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
r
L-thyroxine + 2-oxoglutarate
-
639937
Oryctolagus cuniculus
3-[4-(4-hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
-
-
-
r
L-tryptophan + 2-oxoglutarate
low activtiy
639937
Oryctolagus cuniculus
3-indole-2-oxopropanoate + L-glutamate
-
-
-
r
L-tyrosine + 2-oxoglutarate
low activtiy
639937
Oryctolagus cuniculus
2-oxo-3-phenylpropanoate + L-glutamate
-
-
-
r
additional information
specific for 2-oxoglutarate
639937
Oryctolagus cuniculus
?
-
-
-
-
additional information
pyruvate and oxalacetate are ineffective with 3,5-dinitro-L-tyrosine and only partially effective with iodinated tyrosines
639937
Oryctolagus cuniculus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 48000, SDS-PAGE
Oryctolagus cuniculus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0233
-
L-thyroxine
pH 7.8, 37°C
Oryctolagus cuniculus
0.0317
-
L-triiodothyronine
pH 7.8, 37°C
Oryctolagus cuniculus
1.1
-
3,5-diiodo-L-tyrosine
pH 7.8, 37°C
Oryctolagus cuniculus
3.68
-
3-iodo-L-tyrosine
pH 7.8, 37°C
Oryctolagus cuniculus
6.6
-
3,5-diiodo-L-tyrosine
pH 7.8, 37°C
Oryctolagus cuniculus
39.9
-
3,5-Dinitro-L-tyrosine
pH 7.8, 37°C
Oryctolagus cuniculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
8.6
broad optimum
Oryctolagus cuniculus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
8.6
pH 7.0: 30% of maximal activity with L-triiodothyronine, 15% of maximal activity with 3,5-dinitro-L-tyrosine, pH 7.4-8.6: maximal activity with triiodothyronine and 3,5-dinitro-L-tyrosine
Oryctolagus cuniculus
Other publictions for EC 2.6.1.26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
639937
Soffer
L-Triiodothyronine aminotransf ...
Oryctolagus cuniculus
J. Biol. Chem.
248
1224-1230
1973
1
-
-
-
-
-
1
8
1
-
2
1
-
1
-
-
1
-
-
7
2
1
16
1
-
-
-
-
6
1
1
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
8
1
-
2
1
-
-
-
1
-
7
2
1
16
1
-
-
-
6
1
1
-
-
-
-
-
-
-
-