Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluorouracil | - |
Rattus norvegicus | |
5-Iodouracil | - |
Rattus norvegicus | |
6-Azathymine | - |
Rattus norvegicus | |
6-Azauracil | - |
Rattus norvegicus | |
gabaculine | - |
Rattus norvegicus | |
additional information | not inhibitory: 6-azauridine, 6-azauridine 5-phosphate | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
beta-Alanine | pH 8.8, 37°C | Rattus norvegicus | |
2.7 | - |
(S)-3-amino-2-methylpropanoate | pH 8.8, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.047 | - |
kidney enzyme, pH 8.8, 37°C | Rattus norvegicus |
0.096 | - |
brain enzyme, pH 8.8, 37°C | Rattus norvegicus |
0.133 | - |
liver enzyme, pH 8.8, 37°C | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-25°C, in 0.1 M phosphate buffer, pH 7.0, 1mM EDTA, 2 mM 2-mercaptoethanol, 0.04 mM pyridoxal-5'-phosphate, few days, 50% loss of activity | Rattus norvegicus |
4°C, in 0.1 M phosphate buffer, pH 7.0, 1mM EDTA, 2 mM 2-mercaptoethanol, 0.04 mM pyridoxal-5'-phosphate, 1 week, no loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-amino-2-methylpropanoate + pyruvate | - |
Rattus norvegicus | D-methylmalonate semialdehyde + L-alanine | - |
r | |
4-aminobutanoate + 2-oxoglutarate | transamination at 150% the rate of (S)-3-amino-2-methylpropanoate | Rattus norvegicus | succinic semialdehyde + L-glutamate | - |
r | |
5-aminopentanoate + 2-oxoglutarate | transamination at 145% the rate of (S)-3-amino-2-methylpropanoate | Rattus norvegicus | 5-oxopentanoate + L-glutamate | - |
r | |
beta-alanine + 2-oxoglutarate | as good as (S)-3-amino-2-methylpropanoate | Rattus norvegicus | malonic semialdehyde + L-glutamate | - |
r | |
additional information | no substrates are glyoxylate, pyruvate, oxalacetate, phenylpyruvate | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 x 56000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.2 | - |
- |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0071 | - |
gabaculine | pH 8.8, 37°C | Rattus norvegicus | |
0.7 | - |
6-Azauracil | pH 8.8, 37°C | Rattus norvegicus | |
1.8 | - |
5-fluorouracil | uncompetitive against 2-oxoglutarate, pH 8.8, 37°C | Rattus norvegicus | |
1.9 | - |
5-fluorouracil | competitive to beta-alanine, pH 8.8, 37°C | Rattus norvegicus |