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Literature summary for 2.6.1.2 extracted from

  • Duff, S.M.; Rydel, T.J.; McClerren, A.L.; Zhang, W.; Li, J.Y.; Sturman, E.J.; Halls, C.; Chen, S.; Zeng, J.; Peng, J.; Kretzler, C.N.; Evdokimov, A.
    The enzymology of alanine aminotransferase (AlaAT) isoforms from Hordeum vulgare and other organisms, and the HvAlaAT crystal structure (2012), Arch. Biochem. Biophys., 528, 90-101.
    View publication on PubMed
Search for more literature for 2.6.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Saccharomyces cerevisiae
expressed in Escherichia coli as a His-tagged fusion protein Zea mays
expressed in Escherichia coli as a His-tagged fusion protein Rhodopseudomonas palustris
expressed in Escherichia coli as a His-tagged fusion protein Pseudomonas putida
expressed in Escherichia coli as a His-tagged fusion protein Oryza sativa
expressed in Escherichia coli as a His-tagged fusion protein Halalkalibacterium halodurans
expressed in Escherichia coli as a His-tagged fusion protein Hordeum vulgare

Crystallization (Commentary)

Crystallization (Comment) Organism
HvAlaAT is crystallized in complex with pyridoxal 5'-phosphate and L-cycloserine. Structure of this complex is solved at 2.7 A resolution Hordeum vulgare

Inhibitors

Inhibitors Comment Organism Structure
(aminooxy)acetate
-
 Hordeum vulgare
(aminooxy)acetate
-
 Saccharomyces cerevisiae
cycloserine
-
 Hordeum vulgare
cycloserine
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 2-oxoglutarate 25°C, pH 8.0 Pseudomonas putida
0.08
-
 pyruvate 25°C, pH 8.0 Rhodopseudomonas palustris
0.08
-
 pyruvate 25°C, pH 8.0 Halalkalibacterium halodurans
0.11
-
 pyruvate 25°C, pH 8.0 Oryza sativa
0.2
-
 pyruvate 25°C, pH 8.0 Pseudomonas putida
0.2
-
 pyruvate 25°C, pH 8.0 Hordeum vulgare
0.2
-
 2-oxoglutarate 25°C, pH 8.0 Saccharomyces cerevisiae
0.2
-
 2-oxoglutarate 25°C, pH 8.0 Zea mays
0.2
-
 2-oxoglutarate 25°C, pH 8.0 Rhodopseudomonas palustris
0.2
-
 L-glutamate 25°C, pH 8.0 Rhodopseudomonas palustris
0.21
-
 L-alanine 25°C, pH 8.0 Pseudomonas putida
0.24
-
 L-glutamate 25°C, pH 8.0 Saccharomyces cerevisiae
0.25
-
 2-oxoglutarate 25°C, pH 8.0 Oryza sativa
0.3
-
 2-oxoglutarate 25°C, pH 8.0 Hordeum vulgare
0.32
-
 2-oxoglutarate 25°C, pH 8.0 Halalkalibacterium halodurans
0.37
-
 2-oxoglutarate 25°C, pH 8.0 Zea mays
0.4
-
 pyruvate 25°C, pH 8.0 Saccharomyces cerevisiae
0.4
-
 L-alanine 25°C, pH 8.0 Oryza sativa
0.42
-
 pyruvate 25°C, pH 8.0 Zea mays
0.44
-
 L-glutamate 25°C, pH 8.0 Halalkalibacterium halodurans
0.54
-
 pyruvate 25°C, pH 8.0 Zea mays
0.55
-
 L-glutamate 25°C, pH 8.0 Zea mays
0.56
-
 L-glutamate 25°C, pH 8.0 Zea mays
0.57
-
 L-glutamate 25°C, pH 8.0 Pseudomonas putida
0.62
-
 L-alanine 25°C, pH 8.0 Zea mays
0.68
-
 L-glutamate 25°C, pH 8.0 Oryza sativa
0.8
-
 L-glutamate 25°C, pH 8.0 Hordeum vulgare
1.5 2 L-alanine 25°C, pH 8.0 Rhodopseudomonas palustris
3.06
-
 L-alanine 25°C, pH 8.0 Zea mays
3.6
-
 L-alanine 25°C, pH 8.0 Saccharomyces cerevisiae
3.8
-
 L-alanine 25°C, pH 8.0 Hordeum vulgare
5.15
-
 L-alanine 25°C, pH 8.0 Halalkalibacterium halodurans

Organism

Organism UniProt Comment Textmining
Halalkalibacterium halodurans
-
-
-
Hordeum vulgare P52894
-
-
Oryza sativa
-
-
-
Pseudomonas putida
-
-
-
Rhodopseudomonas palustris
-
-
-
Saccharomyces cerevisiae
-
-
-
Zea mays
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + 2-oxoglutarate
-
 Saccharomyces cerevisiae pyruvate + L-glutamate
-
 r
L-alanine + 2-oxoglutarate
-
 Zea mays pyruvate + L-glutamate
-
 r
L-alanine + 2-oxoglutarate
-
 Rhodopseudomonas palustris pyruvate + L-glutamate
-
 r
L-alanine + 2-oxoglutarate
-
 Pseudomonas putida pyruvate + L-glutamate
-
 r
L-alanine + 2-oxoglutarate
-
 Oryza sativa pyruvate + L-glutamate
-
 r
L-alanine + 2-oxoglutarate
-
 Halalkalibacterium halodurans pyruvate + L-glutamate
-
 r
L-alanine + 2-oxoglutarate
-
 Hordeum vulgare pyruvate + L-glutamate
-
 r
additional information enzyme is also able to utilize aspartate and oxaloacetate with 10% efficiency as compared to the native substrates Hordeum vulgare ?
-
 ?
pyruvate + L-glutamate
-
 Hordeum vulgare L-alanine + 2-oxoglutarate
-
 r

Synonyms

Synonyms Comment Organism
Bh D-AlaAT
-
 Halalkalibacterium halodurans
HvAlaAT
-
 Hordeum vulgare
OsAlaAT
-
 Oryza sativa
Pp beta-AlaAT
-
 Pseudomonas putida
Rp D-AlaAT
-
 Rhodopseudomonas palustris
ScAlaAT
-
 Saccharomyces cerevisiae
ZmAlaAT1
-
 Zea mays
ZmAlaAT2
-
 Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
 L-alanine cofactor: 2-oxoglutarate, 25°C, pH 8.0 Pseudomonas putida
0.08
-
 2-oxoglutarate cofactor: L-alanine, 25°C, pH 8.0 Pseudomonas putida
0.17
-
 pyruvate cofactor: L-glutamate, 25°C, pH 8.0 Pseudomonas putida
0.17
-
 L-glutamate cofactor: pyruvate, 25°C, pH 8.0 Pseudomonas putida
0.33
-
 L-alanine cofactor: 2-oxoglutarate, 25°C, pH 8.0 Zea mays
0.33
-
 2-oxoglutarate cofactor: L-alanine, 25°C, pH 8.0 Zea mays
0.38
-
 L-alanine cofactor: 2-oxoglutarate, 25°C, pH 8.0 Rhodopseudomonas palustris
0.38
-
 2-oxoglutarate cofactor: L-alanine, 25°C, pH 8.0 Rhodopseudomonas palustris
0.67
-
 pyruvate cofactor: L-glutamate, 25°C, pH 8.0 Zea mays
0.67
-
 L-glutamate cofactor: pyruvate, 25°C, pH 8.0 Zea mays
1.25
-
 L-alanine cofactor: 2-oxoglutarate, 25°C, pH 8.0 Halalkalibacterium halodurans
1.25
-
 2-oxoglutarate cofactor: L-alanine, 25°C, pH 8.0 Halalkalibacterium halodurans
2.8
-
 pyruvate cofactor: L-glutamate, 25°C, pH 8.0 Halalkalibacterium halodurans
2.8
-
 L-glutamate cofactor: pyruvate, 25°C, pH 8.0 Halalkalibacterium halodurans
3.06
-
 pyruvate cofactor: L-glutamate, 25°C, pH 8.0 Rhodopseudomonas palustris
3.06
-
 L-glutamate cofactor: pyruvate, 25°C, pH 8.0 Rhodopseudomonas palustris
4.98
-
 L-alanine cofactor: 2-oxoglutarate, 25°C, pH 8.0 Oryza sativa
4.98
-
 2-oxoglutarate cofactor: L-alanine, 25°C, pH 8.0 Oryza sativa
5.49
-
 L-alanine cofactor: 2-oxoglutarate, 25°C, pH 8.0 Zea mays
5.49
-
 2-oxoglutarate cofactor: L-alanine, 25°C, pH 8.0 Zea mays
6.09
-
 L-alanine cofactor: 2-oxoglutarate, 25°C, pH 8.0 Saccharomyces cerevisiae
6.09
-
 2-oxoglutarate cofactor: L-alanine, 25°C, pH 8.0 Saccharomyces cerevisiae
6.56
-
 pyruvate cofactor: L-glutamate, 25°C, pH 8.0 Zea mays
6.56
-
 L-glutamate cofactor: pyruvate, 25°C, pH 8.0 Zea mays
6.86
-
 pyruvate cofactor: L-glutamate, 25°C, pH 8.0 Oryza sativa
6.86
-
 L-glutamate cofactor: pyruvate, 25°C, pH 8.0 Oryza sativa
8.64
-
 pyruvate cofactor: L-glutamate, 25°C, pH 8.0 Saccharomyces cerevisiae
8.64
-
 L-glutamate cofactor: pyruvate, 25°C, pH 8.0 Saccharomyces cerevisiae
12.11
-
 L-alanine cofactor: 2-oxoglutarate, 25°C, pH 8.0 Hordeum vulgare
12.11
-
 2-oxoglutarate cofactor: L-alanine, 25°C, pH 8.0 Hordeum vulgare
25.62
-
 pyruvate cofactor: L-glutamate, 25°C, pH 8.0 Hordeum vulgare
25.62
-
 L-glutamate cofactor: pyruvate, 25°C, pH 8.0 Hordeum vulgare

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Saccharomyces cerevisiae
pyridoxal 5'-phosphate
-
 Zea mays
pyridoxal 5'-phosphate
-
 Rhodopseudomonas palustris
pyridoxal 5'-phosphate
-
 Pseudomonas putida
pyridoxal 5'-phosphate
-
 Oryza sativa
pyridoxal 5'-phosphate
-
 Halalkalibacterium halodurans
pyridoxal 5'-phosphate
-
 Hordeum vulgare

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0069
-
 25°C, pH 8.0 Hordeum vulgare (aminooxy)acetate
0.119
-
 25°C, pH 8.0 Hordeum vulgare cycloserine