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Literature summary for 2.6.1.2 extracted from

  • Vedavathi, M.; Girish, K.S.; Kumar, M.K.
    A novel low molecular weight alanine aminotransferase from fasted rat liver (2006), Biochemistry, 71, S105-S112.
No PubMed abstract available
Search for more literature for 2.6.1.2

Inhibitors

Inhibitors Comment Organism Structure
aminooxyacetic acid 2.5 mM, complete inhibition Rattus norvegicus
Glutarate 2.5 mM, 69% inhibition Rattus norvegicus
HgCl2 2.5 mM, 96% inhibition Rattus norvegicus
hydroxylamine 2.5 mM, complete inhibition Rattus norvegicus
Maleate 2.5 mM, 70% inhibition Rattus norvegicus
additional information not inhibited by EDTA, o-phenanthroline and L-ascorbate Rattus norvegicus
p-hydroxymercuribenzoate 2.5 mM, complete inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 2-oxoglutarate 200 mM L-alanine and 2 mM 2-oxoglutarate in 0.1 M phosphate buffer, pH 7.4, at 37°C Rattus norvegicus
0.51
-
 L-alanine 200 mM L-alanine and 2 mM 2-oxoglutarate in 0.1 M phosphate buffer, pH 7.4, at 37°C Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17700
-
 SDS-PAGE Rattus norvegicus
18300
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sephadex G-150 column chromatography, DEAE-Sephadex A-25 column chromatography, and hydroxyapatite column chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-alanine + 2-oxoglutarate 63% specific activity compared to L-alanine Rattus norvegicus pyruvate + L-glutamate
-
 ?
L-alanine + 2-oxoglutarate
-
 Rattus norvegicus pyruvate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate 6% specific activity compared to L-alanine Rattus norvegicus 2-oxobutan-1,4-dioate + L-glutamate
-
 ?
L-leucine + 2-oxoglutarate 9% specific activity compared to L-alanine Rattus norvegicus 2-oxo-4-methylpentanoate + L-glutamate
-
 ?
L-tyrosine + 2-oxoglutarate 12.5% specific activity compared to L-alanine Rattus norvegicus (4-hydroxyphenyl)pyruvate + L-glutamate
-
 ?
additional information no specificity for L-glutamate, L-cysteine, L-glycine, L-methionine, L-isoleucine, L-threonine, L-valine, L-histidine, beta-alanine, L-arginine hydrochloride, L-cysteine hydrochloride, L-hydroxyproline, DL-phenylalanine, DL-threonine, DL-aspartate, DL-isoleucine, DL-tryptophan, DL-norleucine, DL-histidine hydrochloride, DL-homocysteine, L-beta-phenylalanine Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
AlaAT
-
 Rattus norvegicus
alanine-2-oxoglutarate aminotransferase
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.1
-
 L-alanine 200 mM L-alanine and 2 mM 2-oxoglutarate in 0.1 M phosphate buffer, pH 7.4, at 37°C Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.5
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate activates the enzyme with a Km of 0.057 mM Rattus norvegicus

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus isoelectrofocusing
-
 4.2