Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | - |
Rattus norvegicus | |
hydroxylamine | 1 mM, 96% inhibition | Rattus norvegicus | |
Isonicotinic hydrazide | - |
Rattus norvegicus | |
KCN | - |
Rattus norvegicus | |
Semicarbazide | 1 mM, 40% inhibition | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
112000 | - |
sucrose density gradient centrifugation | Rattus norvegicus |
112000 | 118000 | gel filtration | Homo sapiens |
112000 | 118000 | gel filtration | Canis lupus familiaris |
112000 | 118000 | gel filtration | Felis catus |
115000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | - |
- |
- |
Felis catus | - |
- |
- |
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
heat treatment, ammonium sulfate, DEAE-cellulose, isoelectric focusing, Sephadex G-150, hydroxylapatite, DEAE-cellulose | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Canis lupus familiaris | - |
liver | - |
Felis catus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
480 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, 50 mM potassium phosphate buffer, pH 7.5, 5 mM 2-mercaptoethanol, 5% glycerol, stable for at least 5 weeks | Rattus norvegicus |
0-5°C, 50 mM potassium phosphate buffer, pH 7.5, 5 mM 2-mercaptoethanol, 5% glycerol, stable for 2 weeks | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + alanine | 70% of activity with glutamate | Rattus norvegicus | glycine + pyruvate | - |
ir | |
glyoxylate + glutamate | - |
Rattus norvegicus | glycine + 2-oxoglutarate | - |
ir | |
glyoxylate + glutamine | 42% of activity with glutamate | Rattus norvegicus | glycine + 2-oxosuccinamate | - |
? | |
glyoxylate + methionine | 37% of activity with glutamate | Rattus norvegicus | glycine + 4-methylsulfanyl-2-oxobutanoate | - |
? | |
L-alanine + 2-oxoglutarate | - |
Homo sapiens | pyruvate + L-glutamate | - |
? | |
L-alanine + 2-oxoglutarate | - |
Canis lupus familiaris | pyruvate + L-glutamate | - |
? | |
L-alanine + 2-oxoglutarate | - |
Felis catus | pyruvate + L-glutamate | - |
? | |
L-alanine + 2-oxoglutarate | with 2-oxoglutarate specific for L-alanine | Rattus norvegicus | pyruvate + L-glutamate | - |
? | |
L-alanine + 2-oxoglutarate | glutamate-glyoxylate aminotransferase is identical with alanine-2-oxoglutarate aminotransferase | Rattus norvegicus | pyruvate + L-glutamate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Canis lupus familiaris |
37 | - |
assay at | Felis catus |