Cloned (Comment) | Organism |
---|---|
recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Variovorax paradoxus |
Protein Variants | Comment | Organism |
---|---|---|
D392K | site-directed mutagenesis, slightly reduced activity compared to wild-type enzyme | Variovorax paradoxus |
E345F | site-directed mutagenesis, reduced activity compared to wild-type enzyme | Variovorax paradoxus |
E391K | site-directed mutagenesis, reduced activity compared to wild-type enzyme | Variovorax paradoxus |
G165M | best DELTADELTAG prediction, the mutant shows a 3.3fold reduction in enzymatic activity and an only a slight improvement in the Tm value, which stresses the importance of experimental evaluation of the theoretical data | Variovorax paradoxus |
G165M | site-directed mutagenesis, reduced activity compared to wild-type enzyme | Variovorax paradoxus |
G98M | site-directed mutagenesis, slightly reduced activity compared to wild-type enzyme | Variovorax paradoxus |
G98M/D392K | site-directed mutagenesis, reduced activity compared to wild-type enzyme | Variovorax paradoxus |
G98M/E345F | site-directed mutagenesis, reduced activity compared to wild-type enzyme | Variovorax paradoxus |
additional information | synthesis and optical resolution of beta-phenylalanine and other important aromatic beta-amino acids by biotransformation utilizing an omega-transaminase (omega-TA) from Variovorax paradoxus. Design of mutant variants of the omega-TA to gain higher process stability on the basis of predictions calculated by using the FoldX software. Thermostabilization of a nonthermostable S-selective omega-TA by FoldX-guided site-directed mutagenesis. The melting point (Tm) of the best-performing mutant is increased to 59.3°C, an increase of 4.0°C relative to the Tm value of the wild-type enzyme, whereby the mutant fully retains its specific activity | Variovorax paradoxus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamics of wild-type and mutant enzymes | Variovorax paradoxus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + beta-alanine | Variovorax paradoxus | - |
L-alanine + 3-oxopropanoate | - |
r | |
pyruvate + beta-phenylalanine | Variovorax paradoxus | - |
L-alanine + 3-oxo-phenylpropanoate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Variovorax paradoxus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration | Variovorax paradoxus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-alanine + 3-oxopropanoate = pyruvate + beta-alanine | reaction mechanism with beta-phenylalanine as an amino donor and 2-oxoglutarate as an acceptor, detailed overview | Variovorax paradoxus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24 | - |
purified recombinant mutant G98M, pH 7.2, 40°C, substrate rac-beta-phenylalanine with 2-oxoglutarate | Variovorax paradoxus |
26 | - |
purified recombinant wild-type enzyme, pH 7.2, 40°C, substrate rac-beta-phenylalanine with 2-oxoglutarate | Variovorax paradoxus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + (S)-beta-phenylalanine | - |
Variovorax paradoxus | L-glutamate + 3-oxo-phenylpropanoate | - |
r | |
2-oxoglutarate + rac-beta-phenylalanine | - |
Variovorax paradoxus | L-glutamate + 3-oxo-phenylpropanoate | - |
r | |
pyruvate + beta-alanine | - |
Variovorax paradoxus | L-alanine + 3-oxopropanoate | - |
r | |
pyruvate + beta-phenylalanine | - |
Variovorax paradoxus | L-alanine + 3-oxo-phenylpropanoate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
omega-transaminase | - |
Variovorax paradoxus |
omega-VpTA | - |
Variovorax paradoxus |
S-selective omega-TA | - |
Variovorax paradoxus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Variovorax paradoxus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
54.3 | - |
Tm of recombinant mutant enzyme E391K | Variovorax paradoxus |
55.3 | - |
Tm of recombinant wild-type | Variovorax paradoxus |
56.2 | - |
Tm of recombinant mutant enzyme E345F | Variovorax paradoxus |
56.7 | - |
Tm of recombinant mutant enzyme D392K | Variovorax paradoxus |
58.8 | - |
Tm of recombinant mutant enzyme G98M/D345F | Variovorax paradoxus |
59.3 | - |
Tm of recombinant mutant enzyme G98M | Variovorax paradoxus |
59.4 | - |
Tm of recombinant mutant enzyme G98M/E392K | Variovorax paradoxus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Variovorax paradoxus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | activity range of wild-type enzyme, inactive above | Variovorax paradoxus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | PLP | Variovorax paradoxus |
General Information | Comment | Organism |
---|---|---|
additional information | structure-function analysis, overview | Variovorax paradoxus |