BRENDA - Enzyme Database show
show all sequences of 2.6.1.18

Deracemization of unnatural amino acid: homoalanine using D-amino acid oxidase and omega-transaminase

Seo, Y.M.; Mathew, S.; Bea, H.S.; Khang, Y.H.; Lee, S.H.; Kim, B.G.; Yun, H.; Org. Biomol. Chem. 10, 2482-2485 (2012)

Data extracted from this reference:

Application
Application
Commentary
Organism
biotechnology
an alternative deracemization method for the efficient production of L-homoalanine using D-amino acid oxidase (vide infra) and omega-TA is proposed
Vibrio fluvialis
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Vibrio fluvialis
Inhibitors
Inhibitors
Commentary
Organism
Structure
benzaldehyde
-
Vibrio fluvialis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Vibrio fluvialis
-
-
-
Purification (Commentary)
Commentary
Organism
using an Ni-NTA affinity column
Vibrio fluvialis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxobutyrate + benzylamine
enzyme inhibition by benzaldehyde is the major hurdle in carrying out the omega-TA reaction efficiently. To overcome the product inhibition, a biphasic reaction system in which an organic solvent is employed to extract the inhibitory benzaldehyde from an aqueous system is used
723317
Vibrio fluvialis
L-homoalanine + benzaldehyde
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Vibrio fluvialis
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Vibrio fluvialis
Application (protein specific)
Application
Commentary
Organism
biotechnology
an alternative deracemization method for the efficient production of L-homoalanine using D-amino acid oxidase (vide infra) and omega-TA is proposed
Vibrio fluvialis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Vibrio fluvialis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Vibrio fluvialis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
benzaldehyde
-
Vibrio fluvialis
Purification (Commentary) (protein specific)
Commentary
Organism
using an Ni-NTA affinity column
Vibrio fluvialis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxobutyrate + benzylamine
enzyme inhibition by benzaldehyde is the major hurdle in carrying out the omega-TA reaction efficiently. To overcome the product inhibition, a biphasic reaction system in which an organic solvent is employed to extract the inhibitory benzaldehyde from an aqueous system is used
723317
Vibrio fluvialis
L-homoalanine + benzaldehyde
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Vibrio fluvialis
Other publictions for EC 2.6.1.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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18
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18
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737842
Fuchs
Synthesis of (R)- or (S)-valin ...
Arabidopsis thaliana, Bacillus megaterium, Fusarium graminearum
Bioorg. Med. Chem.
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5558-5562
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737864
Han
Metabolically driven equilibri ...
Ochrobactrum anthropi
Biosci. Biotechnol. Biochem.
78
1788-1790
2014
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1
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721915
Cardenas-Fernandez
Immobilization of Escherichia ...
Escherichia coli
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28
693-698
2012
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1
1
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722376
Malik
-
omega-Transaminase-catalyzed k ...
Aspergillus terreus, Ochrobactrum anthropi
Green Chem.
14
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2012
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723317
Seo
Deracemization of unnatural am ...
Vibrio fluvialis
Org. Biomol. Chem.
10
2482-2485
2012
-
1
1
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1
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1
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721893
Bea
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Asymmetric synthesis of (R)-3- ...
Vibrio fluvialis
Biotechnol. Bioprocess Eng.
16
291-296
2011
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1
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1
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684253
Hoehne
-
A protection strategy substant ...
Achromobacter denitrificans, Vibrio fluvialis
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350
807-812
2008
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12
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12
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671123
Sayer
Crystallization and preliminar ...
Chromobacterium violaceum
Acta Crystallogr. Sect. F
63
117-119
2007
-
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1
1
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684207
Sayer
Crystallization and preliminar ...
Chromobacterium violaceum
Acta Crystallogr. Sect. F
F63
117-119
2007
-
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1
1
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672713
Ingram
One-pot synthesis of amino-alc ...
Pseudomonas aeruginosa
Biotechnol. Bioeng.
96
559-569
2006
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1
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1
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8
1
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657597
Yun
omega-Amino acid:pyruvate tran ...
Achromobacter denitrificans, Achromobacter denitrificans Y2k-2
Appl. Environ. Microbiol.
70
2529-2534
2004
-
2
1
-
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3
3
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2
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4
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1
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1
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20
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1
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1
1
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20
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658326
Yun
Simultaneous synthesis of enan ...
Vibrio fluvialis, Vibrio fluvialis JS17
Biotechnol. Lett.
25
809-814
2003
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1
1
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2
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636597
Ito
Influence of dietary protein l ...
Rattus norvegicus
J. Nutr. Sci. Vitaminol.
47
275-282
2001
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636598
West
Role of cytosine deaminase and ...
Burkholderia cepacia
Antonie van Leeuwenhoek
77
1-5
2000
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636596
Nakano
Two omega-amino acid transamin ...
Bacillus cereus, Bacillus cereus K-22
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81
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1977
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15
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636595
Stinson
beta alanine aminotransferase ...
Phaseolus vulgaris
Biochem. Biophys. Res. Commun.
34
120-127
1969
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288059
Hayaishi
Enzymatic studies on the metab ...
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236
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1961
1
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