Application | Comment | Organism |
---|---|---|
biotechnology | an alternative deracemization method for the efficient production of L-homoalanine using D-amino acid oxidase (vide infra) and omega-TA is proposed | Vibrio fluvialis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Vibrio fluvialis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzaldehyde | - |
Vibrio fluvialis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio fluvialis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using an Ni-NTA affinity column | Vibrio fluvialis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutyrate + benzylamine | enzyme inhibition by benzaldehyde is the major hurdle in carrying out the omega-TA reaction efficiently. To overcome the product inhibition, a biphasic reaction system in which an organic solvent is employed to extract the inhibitory benzaldehyde from an aqueous system is used | Vibrio fluvialis | L-homoalanine + benzaldehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
omega-TA | - |
Vibrio fluvialis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Vibrio fluvialis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Vibrio fluvialis |