BRENDA - Enzyme Database show
show all sequences of 2.6.1.18

Immobilization of Escherichia coli containing omega-transaminase activity in LentiKats

Cardenas-Fernandez, M.; Neto, W.; Lopez, C.; Alvaro, G.; Tufvesson, P.; Woodley, J.M.; Biotechnol. Prog. 28, 693-698 (2012)

Data extracted from this reference:

Application
Application
Commentary
Organism
biotechnology
recombinant Escherichia coli cells containing overexpressed omega-TA activity are immobilized by entrapment in LentiKats and the effect of permeabilization on free cells and their immobilized counterparts is analyzed. Cells are applied in the synthesis of the aromatic amines 3-amino-1-phenylbutane and 1-phenylethylamine. The best results are obtained with CTAB 0.1% for permeabilization which results in an increase in reaction rate by 40% compared to the whole cells. The synthesis of 1-phenylethylamine is carried out using isopropyl amine and L-alanine as amino donors. Using whole cell biocatalysis, the reaction with isopropyl amine is one order of magnitude faster than with L-alanine
Escherichia coli
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-phenylethylamine + pyruvate
-
721915
Escherichia coli
acetophenone + L-alanine
-
-
-
r
4-phenyl-2-butanone + isopropylamine
-
721915
Escherichia coli
3-amino-1-phenylbutane + acetone
-
-
-
ir
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Escherichia coli
Application (protein specific)
Application
Commentary
Organism
biotechnology
recombinant Escherichia coli cells containing overexpressed omega-TA activity are immobilized by entrapment in LentiKats and the effect of permeabilization on free cells and their immobilized counterparts is analyzed. Cells are applied in the synthesis of the aromatic amines 3-amino-1-phenylbutane and 1-phenylethylamine. The best results are obtained with CTAB 0.1% for permeabilization which results in an increase in reaction rate by 40% compared to the whole cells. The synthesis of 1-phenylethylamine is carried out using isopropyl amine and L-alanine as amino donors. Using whole cell biocatalysis, the reaction with isopropyl amine is one order of magnitude faster than with L-alanine
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-phenylethylamine + pyruvate
-
721915
Escherichia coli
acetophenone + L-alanine
-
-
-
r
4-phenyl-2-butanone + isopropylamine
-
721915
Escherichia coli
3-amino-1-phenylbutane + acetone
-
-
-
ir
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Escherichia coli
Other publictions for EC 2.6.1.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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A beta-alanine batabolism path ...
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82
3846-3856
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18
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1
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18
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2
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1
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13
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1
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737842
Fuchs
Synthesis of (R)- or (S)-valin ...
Arabidopsis thaliana, Bacillus megaterium, Fusarium graminearum
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22
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2014
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Metabolically driven equilibri ...
Ochrobactrum anthropi
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1
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721915
Cardenas-Fernandez
Immobilization of Escherichia ...
Escherichia coli
Biotechnol. Prog.
28
693-698
2012
-
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1
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-
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1
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1
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1
1
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2
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1
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1
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722376
Malik
-
omega-Transaminase-catalyzed k ...
Aspergillus terreus, Ochrobactrum anthropi
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5
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2
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2
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723317
Seo
Deracemization of unnatural am ...
Vibrio fluvialis
Org. Biomol. Chem.
10
2482-2485
2012
-
1
1
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1
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1
1
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1
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Bea
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Vibrio fluvialis
Biotechnol. Bioprocess Eng.
16
291-296
2011
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
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684253
Hoehne
-
A protection strategy substant ...
Achromobacter denitrificans, Vibrio fluvialis
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12
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12
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671123
Sayer
Crystallization and preliminar ...
Chromobacterium violaceum
Acta Crystallogr. Sect. F
63
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1
1
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684207
Sayer
Crystallization and preliminar ...
Chromobacterium violaceum
Acta Crystallogr. Sect. F
F63
117-119
2007
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1
1
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2
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1
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672713
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One-pot synthesis of amino-alc ...
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2006
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8
1
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1
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2
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1
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1
1
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1
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2
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1
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8
1
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1
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2
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657597
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omega-Amino acid:pyruvate tran ...
Achromobacter denitrificans, Achromobacter denitrificans Y2k-2
Appl. Environ. Microbiol.
70
2529-2534
2004
-
2
1
-
-
-
3
3
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2
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4
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1
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1
-
20
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1
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2
1
1
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3
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3
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2
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1
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1
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20
-
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658326
Yun
Simultaneous synthesis of enan ...
Vibrio fluvialis, Vibrio fluvialis JS17
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2003
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1
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2
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1
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1
1
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2
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2
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1
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636597
Ito
Influence of dietary protein l ...
Rattus norvegicus
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47
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2001
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636598
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Role of cytosine deaminase and ...
Burkholderia cepacia
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2000
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1
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4
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15
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1
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1
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1
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Stinson
beta alanine aminotransferase ...
Phaseolus vulgaris
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1969
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1961
1
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