BRENDA - Enzyme Database show
show all sequences of 2.6.1.18

One-pot synthesis of amino-alcohols using a de-novo transketolase and beta-alanine:pyruvate transaminase pathway in Escherichia coli

Ingram, C.U.; Bommer, M.; Smith, M.E.; Dalby, P.A.; Ward, J.M.; Hailes, H.C.; Lye, G.J.; Biotechnol. Bioeng. 96, 559-569 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21gold(DE3) cells
Pseudomonas aeruginosa
General Stability
General Stability
Organism
the rate of the reaction with the purified enzyme in 100 mM potassium phosphate buffer, pH 7.0 with 2 mM pyridoxal phosphate cofactor at 25°C slows markedly after 50 h with little further product formation up to 75 h
Pseudomonas aeruginosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
4 * 43000
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-alanine + pyruvate
Pseudomonas aeruginosa
-
malonic semialdehyde + L-alanine
-
-
?
beta-alanine + pyruvate
Pseudomonas aeruginosa PAO2
-
malonic semialdehyde + L-alanine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
-
-
-
Purification (Commentary)
Commentary
Organism
nickel column chromatography
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-alpha-methylbenzylamine + pyruvate
-
672713
Pseudomonas aeruginosa
acetophenone + L-alanine
-
-
-
?
(S)-alpha-methylbenzylamine + pyruvate
-
672713
Pseudomonas aeruginosa PAO2
acetophenone + L-alanine
-
-
-
?
beta-alanine + pyruvate
-
672713
Pseudomonas aeruginosa
malonic semialdehyde + L-alanine
-
-
-
?
beta-alanine + pyruvate
-
672713
Pseudomonas aeruginosa PAO2
malonic semialdehyde + L-alanine
-
-
-
?
L-erythrulose + pyruvate
-
672713
Pseudomonas aeruginosa
2-amino-1,3,4-butanetriol + ?
-
-
-
?
L-erythrulose + pyruvate
-
672713
Pseudomonas aeruginosa PAO2
2-amino-1,3,4-butanetriol + ?
-
-
-
?
additional information
D-methylbenzylamine is not accepted as an amine donor
672713
Pseudomonas aeruginosa
?
-
-
-
-
additional information
D-methylbenzylamine is not accepted as an amine donor
672713
Pseudomonas aeruginosa PAO2
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
4 * 43000
Pseudomonas aeruginosa
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
25
-
the rate of the reaction with the purified enzyme in 100 mM potassium phosphate buffer, pH 7.0 with 2 mM pyridoxal phosphate cofactor at 25°C slows markedly after 50 h with little further product formation up to 75 h
Pseudomonas aeruginosa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
for reactions with (S)-alpha-methylbenzylamine and L-erythrulose
Pseudomonas aeruginosa
8.5
-
above pH 8.5, for reactions with the natural substrates beta-alanine and pyruvate
Pseudomonas aeruginosa
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21gold(DE3) cells
Pseudomonas aeruginosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Pseudomonas aeruginosa
General Stability (protein specific)
General Stability
Organism
the rate of the reaction with the purified enzyme in 100 mM potassium phosphate buffer, pH 7.0 with 2 mM pyridoxal phosphate cofactor at 25°C slows markedly after 50 h with little further product formation up to 75 h
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
4 * 43000
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-alanine + pyruvate
Pseudomonas aeruginosa
-
malonic semialdehyde + L-alanine
-
-
?
beta-alanine + pyruvate
Pseudomonas aeruginosa PAO2
-
malonic semialdehyde + L-alanine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
nickel column chromatography
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-alpha-methylbenzylamine + pyruvate
-
672713
Pseudomonas aeruginosa
acetophenone + L-alanine
-
-
-
?
(S)-alpha-methylbenzylamine + pyruvate
-
672713
Pseudomonas aeruginosa PAO2
acetophenone + L-alanine
-
-
-
?
beta-alanine + pyruvate
-
672713
Pseudomonas aeruginosa
malonic semialdehyde + L-alanine
-
-
-
?
beta-alanine + pyruvate
-
672713
Pseudomonas aeruginosa PAO2
malonic semialdehyde + L-alanine
-
-
-
?
L-erythrulose + pyruvate
-
672713
Pseudomonas aeruginosa
2-amino-1,3,4-butanetriol + ?
-
-
-
?
L-erythrulose + pyruvate
-
672713
Pseudomonas aeruginosa PAO2
2-amino-1,3,4-butanetriol + ?
-
-
-
?
additional information
D-methylbenzylamine is not accepted as an amine donor
672713
Pseudomonas aeruginosa
?
-
-
-
-
additional information
D-methylbenzylamine is not accepted as an amine donor
672713
Pseudomonas aeruginosa PAO2
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 43000
Pseudomonas aeruginosa
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
25
-
the rate of the reaction with the purified enzyme in 100 mM potassium phosphate buffer, pH 7.0 with 2 mM pyridoxal phosphate cofactor at 25°C slows markedly after 50 h with little further product formation up to 75 h
Pseudomonas aeruginosa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
for reactions with (S)-alpha-methylbenzylamine and L-erythrulose
Pseudomonas aeruginosa
8.5
-
above pH 8.5, for reactions with the natural substrates beta-alanine and pyruvate
Pseudomonas aeruginosa
Other publictions for EC 2.6.1.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737507
Wilding
A beta-alanine batabolism path ...
Pseudomonas sp., Pseudomonas sp. AAC
Appl. Environ. Microbiol.
82
3846-3856
2016
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738138
Mathew
Biochemical characterization o ...
Sphaerobacter thermophilus, Sphaerobacter thermophilus DSM 20745
Enzyme Microb. Technol.
87-88
52-60
2016
-
-
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
1
-
13
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
13
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
737842
Fuchs
Synthesis of (R)- or (S)-valin ...
Arabidopsis thaliana, Bacillus megaterium, Fusarium graminearum
Bioorg. Med. Chem.
22
5558-5562
2014
-
3
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737864
Han
Metabolically driven equilibri ...
Ochrobactrum anthropi
Biosci. Biotechnol. Biochem.
78
1788-1790
2014
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721915
Cardenas-Fernandez
Immobilization of Escherichia ...
Escherichia coli
Biotechnol. Prog.
28
693-698
2012
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
722376
Malik
-
omega-Transaminase-catalyzed k ...
Aspergillus terreus, Ochrobactrum anthropi
Green Chem.
14
2137-2140
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
5
-
2
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
723317
Seo
Deracemization of unnatural am ...
Vibrio fluvialis
Org. Biomol. Chem.
10
2482-2485
2012
-
1
1
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
721893
Bea
-
Asymmetric synthesis of (R)-3- ...
Vibrio fluvialis
Biotechnol. Bioprocess Eng.
16
291-296
2011
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
1
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1
-
1
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1
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1
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1
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1
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
684253
Hoehne
-
A protection strategy substant ...
Achromobacter denitrificans, Vibrio fluvialis
Adv. Synth. Catal.
350
807-812
2008
-
-
-
-
-
-
-
-
-
-
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-
-
2
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671123
Sayer
Crystallization and preliminar ...
Chromobacterium violaceum
Acta Crystallogr. Sect. F
63
117-119
2007
-
-
1
1
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
-
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1
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1
1
1
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1
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1
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-
684207
Sayer
Crystallization and preliminar ...
Chromobacterium violaceum
Acta Crystallogr. Sect. F
F63
117-119
2007
-
-
1
1
-
-
-
-
-
-
2
-
-
2
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1
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1
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1
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2
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1
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672713
Ingram
One-pot synthesis of amino-alc ...
Pseudomonas aeruginosa
Biotechnol. Bioeng.
96
559-569
2006
-
-
1
-
-
1
-
-
-
-
1
2
-
3
-
-
1
-
-
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-
8
1
-
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1
-
2
-
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1
-
-
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1
1
-
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1
-
-
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1
2
-
-
-
1
-
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-
-
8
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
657597
Yun
omega-Amino acid:pyruvate tran ...
Achromobacter denitrificans, Achromobacter denitrificans Y2k-2
Appl. Environ. Microbiol.
70
2529-2534
2004
-
2
1
-
-
-
3
3
-
-
-
2
-
4
-
-
1
-
-
-
1
-
20
-
-
-
-
-
-
-
-
1
-
-
-
-
2
1
1
-
-
-
-
3
-
3
-
-
-
2
-
-
-
1
-
-
1
-
20
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658326
Yun
Simultaneous synthesis of enan ...
Vibrio fluvialis, Vibrio fluvialis JS17
Biotechnol. Lett.
25
809-814
2003
-
1
1
-
-
-
2
-
-
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2
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-
2
-
1
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1
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-
1
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1
1
1
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-
2
-
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2
-
1
-
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1
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636597
Ito
Influence of dietary protein l ...
Rattus norvegicus
J. Nutr. Sci. Vitaminol.
47
275-282
2001
-
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3
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3
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3
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636598
West
Role of cytosine deaminase and ...
Burkholderia cepacia
Antonie van Leeuwenhoek
77
1-5
2000
-
-
-
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1
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3
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9
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1
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9
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1
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636596
Nakano
Two omega-amino acid transamin ...
Bacillus cereus, Bacillus cereus K-22
J. Biochem.
81
1375-1381
1977
-
-
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4
1
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2
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1
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15
-
1
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1
-
1
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1
1
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1
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-
-
-
4
-
1
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-
-
-
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1
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15
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
636595
Stinson
beta alanine aminotransferase ...
Phaseolus vulgaris
Biochem. Biophys. Res. Commun.
34
120-127
1969
-
-
-
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-
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1
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1
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1
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1
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1
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288059
Hayaishi
Enzymatic studies on the metab ...
Pseudomonas fluorescens
J. Biol. Chem.
236
781-790
1961
1
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1
2
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1
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1
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1
16
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1
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1
1
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1
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1
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2
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1
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1
16
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1
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1
1
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