BRENDA - Enzyme Database show
show all sequences of 2.6.1.18

Simultaneous synthesis of enantiomerically pure (R)-1-phenylethanol and (R)-alpha-methylbenzylamine from racemic alpha-methylbenzylamine using omega-transaminase/alcohol dehydrogenase/glucose dehydrogenase coupling reaction

Yun, H.; Yang, Y.H.; Cho, B.K.; Hwang, B.Y.; Kim, B.G.; Biotechnol. Lett. 25, 809-814 (2003)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
enzymatic synthesis of enantiomerically pure (R)-1-phenylethanol and (R)-alpha-methylbenzylamine from racemic alpha-methylbenzylamine in a coupled assay using the omega-transaminase, alcohol dehydrogenase, and glucose dehydrogenase
Vibrio fluvialis
Cloned(Commentary)
Commentary
Organism
overexpression of the enzyme in Escherichia coli strain BL21
Vibrio fluvialis
Inhibitors
Inhibitors
Commentary
Organism
Structure
(R)-1-phenylethanol
is a poor inhibitor which reduces enzyme activity by 10% at 25 mM
Vibrio fluvialis
additional information
no inhibition by D-glucose
Vibrio fluvialis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Vibrio fluvialis
-
-
-
Vibrio fluvialis JS17
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-alpha-methylbenzylamine + pyruvate
-
658326
Vibrio fluvialis
acetophenone + alanine
-
-
-
?
(S)-alpha-methylbenzylamine + pyruvate
-
658326
Vibrio fluvialis JS17
acetophenone + alanine
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Vibrio fluvialis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Vibrio fluvialis
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Vibrio fluvialis
Application (protein specific)
Application
Commentary
Organism
synthesis
enzymatic synthesis of enantiomerically pure (R)-1-phenylethanol and (R)-alpha-methylbenzylamine from racemic alpha-methylbenzylamine in a coupled assay using the omega-transaminase, alcohol dehydrogenase, and glucose dehydrogenase
Vibrio fluvialis
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression of the enzyme in Escherichia coli strain BL21
Vibrio fluvialis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
-
Vibrio fluvialis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(R)-1-phenylethanol
is a poor inhibitor which reduces enzyme activity by 10% at 25 mM
Vibrio fluvialis
additional information
no inhibition by D-glucose
Vibrio fluvialis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-alpha-methylbenzylamine + pyruvate
-
658326
Vibrio fluvialis
acetophenone + alanine
-
-
-
?
(S)-alpha-methylbenzylamine + pyruvate
-
658326
Vibrio fluvialis JS17
acetophenone + alanine
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Vibrio fluvialis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Vibrio fluvialis
Other publictions for EC 2.6.1.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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18
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1
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13
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1
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Han
Metabolically driven equilibri ...
Ochrobactrum anthropi
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722376
Malik
-
omega-Transaminase-catalyzed k ...
Aspergillus terreus, Ochrobactrum anthropi
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2012
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5
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2
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2
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723317
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Deracemization of unnatural am ...
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Org. Biomol. Chem.
10
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-
1
1
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721893
Bea
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Asymmetric synthesis of (R)-3- ...
Vibrio fluvialis
Biotechnol. Bioprocess Eng.
16
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2011
-
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1
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1
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1
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1
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1
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684253
Hoehne
-
A protection strategy substant ...
Achromobacter denitrificans, Vibrio fluvialis
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350
807-812
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-
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2
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12
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12
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671123
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Chromobacterium violaceum
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63
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2007
-
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1
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684207
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Chromobacterium violaceum
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F63
117-119
2007
-
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1
1
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2
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2
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672713
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96
559-569
2006
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1
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1
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1
2
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8
1
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1
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8
1
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2
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657597
Yun
omega-Amino acid:pyruvate tran ...
Achromobacter denitrificans, Achromobacter denitrificans Y2k-2
Appl. Environ. Microbiol.
70
2529-2534
2004
-
2
1
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3
3
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2
-
4
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1
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1
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20
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1
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2
1
1
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1
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20
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658326
Yun
Simultaneous synthesis of enan ...
Vibrio fluvialis, Vibrio fluvialis JS17
Biotechnol. Lett.
25
809-814
2003
-
1
1
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2
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2
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2
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1
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1
1
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2
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636597
Ito
Influence of dietary protein l ...
Rattus norvegicus
J. Nutr. Sci. Vitaminol.
47
275-282
2001
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636598
West
Role of cytosine deaminase and ...
Burkholderia cepacia
Antonie van Leeuwenhoek
77
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2000
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9
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Two omega-amino acid transamin ...
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15
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1
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4
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15
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1
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1
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1
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1
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636595
Stinson
beta alanine aminotransferase ...
Phaseolus vulgaris
Biochem. Biophys. Res. Commun.
34
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1969
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288059
Hayaishi
Enzymatic studies on the metab ...
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236
781-790
1961
1
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