Application | Comment | Organism |
---|---|---|
additional information | the enzyme can be utilized as a catalyst for kinetic resolution of beta-amino acids and amines | Achromobacter denitrificans |
synthesis | enzymatic synthesis of enantiomerically pure D-beta-amino-n-butyric acid from racemic beta-amino-n-butyric acid | Achromobacter denitrificans |
Cloned (Comment) | Organism |
---|---|
gene aptA, DNA and amino acid sequence determination and analysis, functional expression of the His-tagged enzyme in Escherichia coli strain BL21 | Achromobacter denitrificans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-amino-2,3-dihydrobenzoic acid | i.e. gabaculine, strong inhibition | Achromobacter denitrificans | |
aminooxyacetic acid | strong inhibition | Achromobacter denitrificans | |
hydroxylamine | strong inhibition | Achromobacter denitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Achromobacter denitrificans | |
11 | - |
pyruvate | recombinant enzyme | Achromobacter denitrificans | |
56 | - |
L-beta-amino-n-butyric acid | recombinant enzyme | Achromobacter denitrificans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-beta-amino-n-butyric acid + pyruvate | Achromobacter denitrificans | - |
3-oxo-n-butyric acid + alanine | - |
? | |
L-beta-amino-n-butyric acid + pyruvate | Achromobacter denitrificans Y2k-2 | - |
3-oxo-n-butyric acid + alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter denitrificans | Q7WWK8 | gene aptA | - |
Achromobacter denitrificans Y2k-2 | Q7WWK8 | gene aptA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 | Achromobacter denitrificans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
77.2 | - |
purified recombinant enzyme | Achromobacter denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-1,2-dimethylpropylamine + pyruvate | 7.5% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans | 3-methyl-2-oxobutane + alanine | - |
? | |
(R,S)-1,2-dimethylpropylamine + pyruvate | 7.5% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans Y2k-2 | 3-methyl-2-oxobutane + alanine | - |
? | |
(R,S)-1,5-dimethylhexylamine + pyruvate | 8.3% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans | 6-methyl-2-oxoheptane + alanine | - |
? | |
(R,S)-1,5-dimethylhexylamine + pyruvate | 8.3% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans Y2k-2 | 6-methyl-2-oxoheptane + alanine | - |
? | |
(R,S)-2-aminoheptane + pyruvate | 9.1% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans | 2-oxoheptane + alanine | - |
? | |
(R,S)-sec-butylamine + pyruvate | 7.3% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans | ? + alanine | - |
? | |
(S)-alpha-methylbenzylamine + pyruvate | 14% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans | acetophenone + alanine | - |
? | |
beta-alanine + pyruvate | 10% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans | malonic semialdehyde + L-alanine | - |
? | |
beta-alanine + pyruvate | 10% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans Y2k-2 | malonic semialdehyde + L-alanine | - |
? | |
butyraldehyde + (S)-alpha-methylbenzylamine | 118% of activity with pyruvate | Achromobacter denitrificans | ? + acetophenone | - |
? | |
glyoxylate + (S)-alpha-methylbenzylamine | 13% of activity with pyruvate | Achromobacter denitrificans | glycine + acetophenone | - |
? | |
L-beta-amino-n-butyric acid + pyruvate | - |
Achromobacter denitrificans | 3-oxo-n-butyric acid + alanine | - |
? | |
L-beta-amino-n-butyric acid + pyruvate | - |
Achromobacter denitrificans Y2k-2 | 3-oxo-n-butyric acid + alanine | - |
? | |
L-beta-amino-n-butyric acid + pyruvate | high activity, highly stereoselective enzyme | Achromobacter denitrificans | 3-oxobutyrate + alanine | - |
? | |
L-beta-homoleucine + pyruvate | 2.6% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans | 5-methyl-3-oxohexanoate + alanine | - |
? | |
L-beta-leucine + pyruvate | 4.3% of activity with L-beta-amino-n-butyric acid | Achromobacter denitrificans | 4-methyl-3-oxopentanoate + alanine | - |
? | |
additional information | substrate specificity, (R,S)-alpha-ethylbenzylamine, L-beta-homoisoleucin, L-3-amino-3-phenylpropanoic acid, (R,S)-1,2-dimethylbutylamine, oxalacetate, alpha-ketoglutarate, and ethylacetoacetate are no substrates | Achromobacter denitrificans | ? | - |
? | |
additional information | substrate specificity, (R,S)-alpha-ethylbenzylamine, L-beta-homoisoleucin, L-3-amino-3-phenylpropanoic acid, (R,S)-1,2-dimethylbutylamine, oxalacetate, alpha-ketoglutarate, and ethylacetoacetate are no substrates | Achromobacter denitrificans Y2k-2 | ? | - |
? | |
propion aldehyde + (S)-alpha-methylbenzylamine | 105% of activity with pyruvate | Achromobacter denitrificans | ? + acetophenone | - |
? | |
pyruvic aldehyde + (S)-alpha-methylbenzylamine | 5% of activity with pyruvate | Achromobacter denitrificans | ? + acetophenone | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AptA | - |
Achromobacter denitrificans |
omega-amino acid:pyruvate transaminase | - |
Achromobacter denitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Achromobacter denitrificans |