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Literature summary for 2.6.1.18 extracted from

  • Yun, H.; Lim, S.; Cho, B.K.; Kim, B.G.
    omega-Amino acid:pyruvate transaminase from Alcaligenes denitrificans Y2k-2: a new catalyst for kinetic resolution of beta-amino acids and amines (2004), Appl. Environ. Microbiol., 70, 2529-2534.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
additional information the enzyme can be utilized as a catalyst for kinetic resolution of beta-amino acids and amines Achromobacter denitrificans
synthesis enzymatic synthesis of enantiomerically pure D-beta-amino-n-butyric acid from racemic beta-amino-n-butyric acid Achromobacter denitrificans

Cloned(Commentary)

Cloned (Comment) Organism
gene aptA, DNA and amino acid sequence determination and analysis, functional expression of the His-tagged enzyme in Escherichia coli strain BL21 Achromobacter denitrificans

Inhibitors

Inhibitors Comment Organism Structure
3-amino-2,3-dihydrobenzoic acid i.e. gabaculine, strong inhibition Achromobacter denitrificans
aminooxyacetic acid strong inhibition Achromobacter denitrificans
hydroxylamine strong inhibition Achromobacter denitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Achromobacter denitrificans
11
-
pyruvate recombinant enzyme Achromobacter denitrificans
56
-
L-beta-amino-n-butyric acid recombinant enzyme Achromobacter denitrificans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-beta-amino-n-butyric acid + pyruvate Achromobacter denitrificans
-
3-oxo-n-butyric acid + alanine
-
?
L-beta-amino-n-butyric acid + pyruvate Achromobacter denitrificans Y2k-2
-
3-oxo-n-butyric acid + alanine
-
?

Organism

Organism UniProt Comment Textmining
Achromobacter denitrificans Q7WWK8 gene aptA
-
Achromobacter denitrificans Y2k-2 Q7WWK8 gene aptA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 Achromobacter denitrificans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
77.2
-
purified recombinant enzyme Achromobacter denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-1,2-dimethylpropylamine + pyruvate 7.5% of activity with L-beta-amino-n-butyric acid Achromobacter denitrificans 3-methyl-2-oxobutane + alanine
-
?
(R,S)-1,2-dimethylpropylamine + pyruvate 7.5% of activity with L-beta-amino-n-butyric acid Achromobacter denitrificans Y2k-2 3-methyl-2-oxobutane + alanine
-
?
(R,S)-1,5-dimethylhexylamine + pyruvate 8.3% of activity with L-beta-amino-n-butyric acid Achromobacter denitrificans 6-methyl-2-oxoheptane + alanine
-
?
(R,S)-1,5-dimethylhexylamine + pyruvate 8.3% of activity with L-beta-amino-n-butyric acid Achromobacter denitrificans Y2k-2 6-methyl-2-oxoheptane + alanine
-
?
(R,S)-2-aminoheptane + pyruvate 9.1% of activity with L-beta-amino-n-butyric acid Achromobacter denitrificans 2-oxoheptane + alanine
-
?
(R,S)-sec-butylamine + pyruvate 7.3% of activity with L-beta-amino-n-butyric acid Achromobacter denitrificans ? + alanine
-
?
(S)-alpha-methylbenzylamine + pyruvate 14% of activity with L-beta-amino-n-butyric acid Achromobacter denitrificans acetophenone + alanine
-
?
beta-alanine + pyruvate 10% of activity with L-beta-amino-n-butyric acid Achromobacter denitrificans malonic semialdehyde + L-alanine
-
?
beta-alanine + pyruvate 10% of activity with L-beta-amino-n-butyric acid Achromobacter denitrificans Y2k-2 malonic semialdehyde + L-alanine
-
?
butyraldehyde + (S)-alpha-methylbenzylamine 118% of activity with pyruvate Achromobacter denitrificans ? + acetophenone
-
?
glyoxylate + (S)-alpha-methylbenzylamine 13% of activity with pyruvate Achromobacter denitrificans glycine + acetophenone
-
?
L-beta-amino-n-butyric acid + pyruvate
-
Achromobacter denitrificans 3-oxo-n-butyric acid + alanine
-
?
L-beta-amino-n-butyric acid + pyruvate
-
Achromobacter denitrificans Y2k-2 3-oxo-n-butyric acid + alanine
-
?
L-beta-amino-n-butyric acid + pyruvate high activity, highly stereoselective enzyme Achromobacter denitrificans 3-oxobutyrate + alanine
-
?
L-beta-homoleucine + pyruvate 2.6% of activity with L-beta-amino-n-butyric acid Achromobacter denitrificans 5-methyl-3-oxohexanoate + alanine
-
?
L-beta-leucine + pyruvate 4.3% of activity with L-beta-amino-n-butyric acid Achromobacter denitrificans 4-methyl-3-oxopentanoate + alanine
-
?
additional information substrate specificity, (R,S)-alpha-ethylbenzylamine, L-beta-homoisoleucin, L-3-amino-3-phenylpropanoic acid, (R,S)-1,2-dimethylbutylamine, oxalacetate, alpha-ketoglutarate, and ethylacetoacetate are no substrates Achromobacter denitrificans ?
-
?
additional information substrate specificity, (R,S)-alpha-ethylbenzylamine, L-beta-homoisoleucin, L-3-amino-3-phenylpropanoic acid, (R,S)-1,2-dimethylbutylamine, oxalacetate, alpha-ketoglutarate, and ethylacetoacetate are no substrates Achromobacter denitrificans Y2k-2 ?
-
?
propion aldehyde + (S)-alpha-methylbenzylamine 105% of activity with pyruvate Achromobacter denitrificans ? + acetophenone
-
?
pyruvic aldehyde + (S)-alpha-methylbenzylamine 5% of activity with pyruvate Achromobacter denitrificans ? + acetophenone
-
?

Synonyms

Synonyms Comment Organism
AptA
-
Achromobacter denitrificans
omega-amino acid:pyruvate transaminase
-
Achromobacter denitrificans

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Achromobacter denitrificans