Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is useful in bioproduction of N-acetylglucosamine (GlcNAc), a nutraceutical that has various applications in healthcare | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | combinatorial fine-tuning of glucosamine-6-phosphate N-acetyltransferase (GNA1) and glutamine-fructose-6-phosphate aminotransferase (GlmS) expression by 5-terminus fusion engineering leads to overproduction of N-acetylglucosamine in Bacillus subtilis. Enhanced expression of GlmS is achieved at the transcriptional and translational levels by fusing an mRNA stabilizer to the 5'-terminus of GlmS gene. Under the control of GNA1 (fusion with cMyc tag and with the optimum RBS M-Rm) and GlmS (fusion with mRNA stabilizer DELTAermC+14/7A), the GlcNAc titer and yield in the shake flask increase to 18.5 g/l and 0.37 g GlcNAc/g glucose, which are 2.9fold and 2.3fold that of the control, respectively. Synthetic pathway fine-tuning method at the transcriptional and translational levels by combinatorial modulation of regulatory elements, including epitope tag, RBS sequence, and mRNA stabilizer, method evaluation, overview | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + D-fructose 6-phosphate | Bacillus subtilis | - |
L-glutamate + D-glucosamine 6-phosphate | - |
? | |
L-glutamine + D-fructose 6-phosphate | Bacillus subtilis 168 | - |
L-glutamate + D-glucosamine 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P0CI73 | - |
- |
Bacillus subtilis 168 | P0CI73 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + D-fructose 6-phosphate | - |
Bacillus subtilis | L-glutamate + D-glucosamine 6-phosphate | - |
? | |
L-glutamine + D-fructose 6-phosphate | - |
Bacillus subtilis 168 | L-glutamate + D-glucosamine 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BsglmS | - |
Bacillus subtilis |
GlmS | - |
Bacillus subtilis |
glutamine-fructose-6-phosphate aminotransferase | - |
Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
physiological function | glucosamine-6-phosphate N-acetyltransferase (GNA1), catalyzing acetyl transfer from acetyl-coenzyme A to glucosamine-6-phosphate (GlcN-6P) and glutamine-fructose-6-phosphate aminotransferase (GlmS) catalyzing the formation of GlcN-6P from fructose-6-phosphate (Fru-6P), are two key enzymes in Bacillus subtilis for the bioproduction of N-acetylglucosamine (GlcNAc) | Bacillus subtilis |